Kazachstania aerobia sp. nov., an ascomycetous yeast species from aerobically deteriorating corn silage

Lu, Hui-Zhong; Cai, Yimin; Wu, Zhongliang; Jia, Jianhua; Bai, Feng‐Yan

doi:10.1099/ijs.0.63257-0

Cited by 40 publications

(40 citation statements)

References 19 publications

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“…Previous studies have shown that the species of Saccharomyces sensu stricto exhibit relatively homogeneous karyotypes compared with those of species of Saccharomyces sensu lato (Vaughan-Martini et al, 1993;Fischer et al, 2000;Naumov et al, 1992Naumov et al, , 1995Petersen et al, 1999;Lu et al, 2004;Wu & Bai, 2005). The electrophoretic karyotypes of strains H-6 T , ZX-15 and ZX-20 bore a superficial resemblance to those of the accepted Saccharomyces species, being consistent with their placement in this genus based on rDNA sequence analysis (Fig.…”

Section: Electrophoretic Karyotypingsupporting

confidence: 65%

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Saccharomyces arboricolus sp. nov., a yeast species from tree bark

Wang

Bai

2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Three ascomycetous yeast strains, H-6 T , ZX-15 and ZX-20, isolated from the bark of two tree species of the family Fagaceae collected from different regions of China, formed unconjugated and persistent asci containing two to four globose ascospores. 26S rDNA D1/D2 domain and internal transcribed spacer (ITS) region (including 5.8S rDNA) sequence analysis showed that they were closely related to the currently accepted Saccharomyces species with strong support. Comparisons of the rDNA sequences, electrophoretic karyotypes and physiological characters indicated that the three strains represent a novel species in the genus Saccharomyces. The name Saccharomyces arboricolus sp. nov. was proposed for the novel species, with H-6 T (5AS 2.3317 T 5CBS 10644 T ) isolated from the bark of Quercus fabri as the type strain.Seven species are currently included in the genus Saccharomyces Meyen ex Reess as redefined recently by Kurtzman (2003), based on multigene sequence analysis (Kurtzman & Robnett, 2003). During the investigation of the diversity of ascomycetous yeasts associated with plant materials from China, three strains, H-6 T , ZX-15 and ZX-20, isolated from the bark of broadleaf trees, were found to represent a novel species of the genus Saccharomyces by physiological characterization, rRNA gene sequencing and electrophoretic karyotyping.Yeast strains living in bark were isolated by using the enrichment method. Pieces of bark with or without exudates from independent trees were cut with a sterile scalpel and placed into sterile plastic tubes containing 3 ml liquid enrichment medium. The medium was made according to Sniegowski et al. (2002) with minor modifications, containing (w/v) 0.3 % yeast extract, 0.3 % malt extract, 0.5 % peptone, 0.5 % sucrose, 0.5 % galactose, 7.6 % (v/v) ethanol, 200 mg chloramphenicol ml 21 and 1 ml 1 M HCl per litre. The cultures were incubated at room temperature without shaking for 7 to 14 days. Aliquots (100 ml) of the 10 22 to 10 24 diluted enrichment culture was spread on plate medium made by adding 2 % agar to the liquid enrichment medium. After 2 days incubation at room temperature or 25 u C, colonies with different morphological characters were transferred into malt extract agar slants for further purification and examination.

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Section: Electrophoretic Karyotypingsupporting

confidence: 65%

“…The DNA fragment covering the ITS region (including 5.8S rDNA) and the large-subunit rDNA D1/D2 domain was amplified and sequenced as described previously (Lu et al, 2004). Molecular phylogenetic analysis was performed by the methods described by Bai et al (2002).…”

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confidence: 99%

Saccharomyces arboricolus sp. nov., a yeast species from tree bark

Wang

Bai

2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…In our study, the dominant yeast species after exposure to air, as observed from the DGGE profiles of fungal DNA and RNA, was Kazachstania exigua, in both the PE and OB silages. Yeasts of the genus of Kazachstania were previously observed in aerobically deteriorating corn silages (21). Furthermore, Pichia kudriavzevii was observed in PE silages after 7 days of air exposure.…”

Section: Vol 77 2011 Aerobic Spoilage Of Corn Silage Under Differenmentioning

confidence: 81%

Microbial Dynamics during Aerobic Exposure of Corn Silage Stored under Oxygen Barrier or Polyethylene Films

Dolci

Tabacco

Cocolin

et al. 2011

Appl Environ Microbiol

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The aims of this study were to compare the effects of sealing forage corn with a new oxygen barrier film with those obtained by using a conventional polyethylene film. This comparison was made during both ensilage and subsequent exposure of silage to air and included chemical, microbiological, and molecular (DNA and RNA) assessments. The forage was inoculated with a mixture of Lactobacillus buchneri, Lactobacillus plantarum, and Enterococcus faecium and ensiled in polyethylene (PE) and oxygen barrier (OB) plastic bags. The oxygen permeability of the PE and OB films was 1,480 and 70 cm 3 m ؊2 per 24 h at 23°C, respectively. The silages were sampled after 110 days of ensilage and after 2, 5, 7, 9, and 14 days of air exposure and analyzed for fermentation characteristics, conventional microbial enumeration, and bacterial and fungal community fingerprinting via PCR-denaturing gradient gel electrophoresis (DGGE) and reverse transcription (RT)-PCR-DGGE. The yeast counts in the PE and OB silages were 3.12 and 1.17 log 10 CFU g ؊1 , respectively, with corresponding aerobic stabilities of 65 and 152 h. Acetobacter pasteurianus was present at both the DNA and RNA levels in the PE silage samples after 2 days of air exposure, whereas it was found only after 7 days in the OB silages. RT-PCR-DGGE revealed the activity of Aspergillus fumigatus in the PE samples from the day 7 of air exposure, whereas it appeared only after 14 days in the OB silages. It has been shown that the use of an oxygen barrier film can ensure a longer shelf life of silage after aerobic exposure.

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“…Since then, different Kazachstania species have been isolated from bamboo litter, mushrooms, and decaying corn silage (16,21,41), but never from grapes or fermenting musts. Here we report the occurrence of Kazachstania sp.…”

Section: Discussionmentioning

confidence: 99%

Yeast Community Structures and Dynamics in Healthy andBotrytis-Affected Grape Must Fermentations

Nisiotou

Spiropoulos

Nychas

2007

Appl Environ Microbiol

110

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Indigenous yeast population dynamics during the fermentation of healthy and Botrytis-affected grape juice samples from two regions in Greece, Attica and Arcadia, were surveyed. Species diversity was evaluated by using restriction fragment length polymorphism and sequence analyses of the 5.8S internal transcribed spacer and the D1/D2 ribosomal DNA (rDNA) regions of cultivable yeasts. Community-level profiles were also obtained by direct analysis of fermenting samples through denaturing gradient gel electrophoresis of 26S rDNA amplicons. Both approaches revealed structural divergences in yeast communities between samples of different sanitary states or geographical origins. In all cases, Botrytis infection severely perturbed the bioprocess of fermentation by dramatically altering species heterogeneity and succession during the time course. At the beginning and middle of fermentations, Botrytis-affected samples possessed higher levels of biodiversity than their healthy counterparts, being enriched with fermentative and/or spoilage species, such as Zygosaccharomyces bailii and Issatchenkia spp. or Kluyveromyces dobzhanskii and Kazachstania sp. populations that have not been reported before for wine fermentations. Importantly, Botrytis-affected samples exposed discrete final species dominance. Selection was not species specific, and two different populations, i.e., Saccharomyces cerevisiae in samples from Arcadia and Z. bailii in samples from Attica, could be recovered at the end of Botrytis-affected fermentations. The governing of wine fermentations by Z. bailii is reported for the first time and could elucidate the origins and role of this particular spoilage microbe for the wine industry. This is the first survey to compare healthy and Botrytis-affected spontaneous fermentations by using both culture-based and -independent molecular methods in an attempt to further illuminate the complex yeast ecology of grape must fermentations.Grape must fermentations are governed by dynamic yeast species assemblages, whose physiognomy is influenced by several chemical constraints and biological attributes. Among these factors, the chemical composition of the must, particular enological practices, and primary species richness/heterogeneity in the grape juice have been shown to determine yeast succession during the fermentation course and the final species dominance (12, 13). Accumulating data reveal additional factors that may designate the boundaries of yeast inhabitants, such as the grape variety and the geographical location of the vineyard (6,12,31,33,38). Biological invasions also influence resident yeast biota and may stimulate forces that shape community diversity, yet their impact on must fermentation has been underestimated in earlier literature (12,20). Botrytis is an important grape pathogen that dramatically changes the physicochemical state of berries and consequently may alter the structure of the yeast community (12,27). It has also been postulated that Botrytis infection may affect species succession during alcoh...

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Kazachstania aerobia sp. nov., an ascomycetous yeast species from aerobically deteriorating corn silage

Cited by 40 publications

References 19 publications

Saccharomyces arboricolus sp. nov., a yeast species from tree bark

Saccharomyces arboricolus sp. nov., a yeast species from tree bark

Microbial Dynamics during Aerobic Exposure of Corn Silage Stored under Oxygen Barrier or Polyethylene Films

Yeast Community Structures and Dynamics in Healthy andBotrytis-Affected Grape Must Fermentations

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