KDM5B overexpression predicts a poor prognosis in patients with squamous cell carcinoma of the head and neck

Huang, Donghai; Qiu, Yuanzheng; Li, Guo; Liu, Chao; She, Li; Zhang, Diekuo; Chen, Xiyu; Zhu, Guanghao; Zhang, Xin; Tian, Yongquan; Liu, Yong

doi:10.7150/jca.22145

Cited by 24 publications

(23 citation statements)

References 31 publications

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“…KDM5A and KDM5B, both of which are encoded on autosomal chromosomes, are candidate oncoproteins (BLAIR et al 2011). They are overexpressed in a number of different cancers including breast, ovarian and lung, and their expression correlates with both proliferation rate and propensity for metastatic invasion (HAYAMI et al 2010;HOU et al 2012;TENG et al 2013;YAMAMOTO et al 2014;WANG et al 2015;FENG et al 2017;HUANG et al 2018). The link between the X-linked KDM5C gene and cancer is less straight-forward, as it has both oncogenic and tumor suppressive activities in tissue-dependent manner (HARMEYER et al 2017).…”

Section: Introductionmentioning

confidence: 99%

The histone demethylase KDM5 is essential for larval growth inDrosophila

Secombe

Drelon

Belálcazar

2018

Preprint

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Regulated gene expression is necessary for developmental and homeostatic processes. The KDM5 family of proteins are histone H3 lysine 4 demethylases that can regulate transcription through both demethylase-dependent and independent mechanisms. While loss and overexpression of KDM5 proteins are linked to intellectual disability and cancer, respectively, their normal developmental functions remain less characterized. Drosophila melanogaster provides an ideal system to investigate KDM5 function, as it encodes a single ortholog in contrast to the four paralogs found in mammalian cells. To examine the consequences of complete loss of KDM5, we generated a null allele of Drosophila kdm5, also known as little imaginal discs (lid), and show that it is essential for development. Animals lacking KDM5 die during late pupal development but show a dramatically delayed larval development that coincides with decreased proliferation and increased cell death in imaginal discs. Interestingly, this developmental delay is independent of the well-characterized Jumonji C (JmjC) domainencoded histone demethylase activity and plant homedomain (PHD) motif-mediated chromatin binding activities of KDM5, suggesting key functions for less characterized domains. Consistent with the phenotypes observed, transcriptome analyses of kdm5 null mutant wing imaginal discs revealed the dysregulation of genes involved in several cellular processes, including cell cycle progression and DNA repair. Together, our data provide the first description of complete loss of KDM5 function in a metazoan and offer an invaluable tool for defining the biological activities of KDM5 family proteins.

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Section: Introductionmentioning

confidence: 99%

The histone demethylase KDM5 is essential for larval growth inDrosophila

Secombe

Drelon

Belálcazar

2018

Preprint

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“…Four‐micrometre thick paraffin‐imbedded tumour sections were initially stained with haematoxylin and eosin to confirm the tumourigenesis of SCCHN cells in vivo. All immunohistochemistry staining was performed as described in our previous studies . Briefly, human SCCHN and xenograft sections were stained with the indicated primary antibodies, followed by sequential incubation of secondary antibody and diaminobenzidine.…”

Section: Methodsmentioning

confidence: 99%

“…All immunohistochemistry staining was performed as described in our previous studies. [19][20][21][22] Briefly, human SCCHN and xenograft sections were stained with the indicated primary antibodies, followed by sequential incubation of secondary antibody and diaminobenzidine. Slides were incubated with immunoglobulin G rather than primary antibodies as negative controls.…”

Section: Haematoxylin and Eosin Staining Immunohistochemistry And mentioning

confidence: 99%

Wnt3a promotes radioresistance via autophagy in squamous cell carcinoma of the head and neck

Jing

Chen

et al. 2019

J Cellular Molecular Medi

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The canonical Wnt/β‐catenin signalling pathway and autophagy play critical roles in cancer progression. However, the role of Wnt‐mediated autophagy in cancer radioresistance remains unclear. In this study, we found that irradiation activated the Wnt/β‐catenin and autophagic signalling pathways in squamous cell carcinoma of the head and neck (SCCHN). Wnt3a is a classical ligand that activated the Wnt/β‐catenin signalling pathway, induced autophagy and decreased the sensitivity of SCCHN to irradiation both in vitro and in vivo. Further mechanistic analysis revealed that Wnt3a promoted SCCHN radioresistance via protective autophagy. Finally, expression of the Wnt3a protein was elevated in both SCCHN tissues and patients' serum. Patients showing high expression of Wnt3a displayed a worse prognosis. Taken together, our study indicates that both the canonical Wnt and autophagic signalling pathways are valuable targets for sensitizing SCCHN to irradiation.

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“…Immunohistochemistry was performed as reported previously [12][13][14]. In brief, antigen retrieval was carried out in 10 mM citrate buffer (pH 6.0) in a microwave oven for 15 min.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Wnt3a protein overexpression predicts worse overall survival in laryngeal squamous cell carcinoma

Zhang

Chen

et al. 2019

J. Cancer

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As a classical ligand in the canonical Wnt/β-catenin signaling pathway, the role of Wnt3a in laryngeal squamous cell carcinoma (LSCC) remains unclear. Therefore, the expression pattern of the Wnt3a protein in 222 primary LSCC, and 19 corresponding adjacent non-carcinoma specimens, was detected by immunohistochemistry and further correlated with clinicopathological parameters. The results showed that LSCC tissue expressed higher levels of the Wnt3a protein when compared to the corresponding adjacent non-cancerous tissues. High expression of Wnt3a was closely related to histological grade (P = 0.031), clinical stage (I+II / III+IV; P = 0.004), and lymph node metastasis (P = 0.03). Kaplan-Meier analysis evidenced that a worse overall survival (OS) was correlated to the group with high Wnt3a expression (P = 0.003). When stratified survival analyses were performed, patients with lymph node metastasis/advanced clinical stages and high Wnt3a expression had worse OS rates than patients with other features (P < 0.001). Finally, multivariate analysis showed that Wnt3a expression was an independent prognosis factor for LSCC patients. The current findings suggest that Wnt3a is tightly related to the LSCC progression and could serve as a valuable clinic biomarker for LSCC patients.

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KDM5B overexpression predicts a poor prognosis in patients with squamous cell carcinoma of the head and neck

Cited by 24 publications

References 31 publications

The histone demethylase KDM5 is essential for larval growth inDrosophila

The histone demethylase KDM5 is essential for larval growth inDrosophila

Wnt3a promotes radioresistance via autophagy in squamous cell carcinoma of the head and neck

Wnt3a protein overexpression predicts worse overall survival in laryngeal squamous cell carcinoma

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