KDM6A promotes chondrogenic differentiation of periodontal ligament stem cells by demethylation of SOX9

Wang, Pingting; Li, Yanjing; Meng, Tao; Zhang, Junjiang; Wei, Yuansong; Meng, Zhaosong; Lin, Yunfeng; Liu, Dayong; Sui, Lei

doi:10.1111/cpr.12413

Cited by 44 publications

(50 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…miR‐204 was reported to function as an inhibitory molecule in oral squamous cell carcinoma by suppressing invasiveness and cancer stemness of tumours via targeting Slug, epithelial‐mesenchymal transition of tumours via Sox4 and initiation or progression of cancers via C‐X‐C motif chemokine receptor 4 . In addition, miR‐204 may also be involved in the chondrogenic differentiation of periodontal ligament stem cells . miR‐513c was reported to inhibit the development of nervous system tumours and hepatocellular carcinoma .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of microRNA‐144‐5p and its putative targets in inflamed gingiva from chronic periodontitis patients

Wang

Chen

et al. 2018

J of Periodontal Research

View full text Add to dashboard Cite

Background and Objective This study aimed to discover the distinctive MicroRNAs (miRNA) functioning in the pathogenesis of periodontal inflammation, which might be potential therapy targets of chronic periodontitis. Material and Methods miRNA profiles of human inflamed gingival tissue from three previous microarrays were re‐analysed. Gingival tissues were collected for the validation of overlapping miRNAs, and a network was constructed to show regulatory connection between overlapping miRNAs and periodontitis‐associated target genes. Potential miRNAs were screened based on their expression levels and predicted target genes. Correlation analysis and binding site prediction were conducted to reveal the relationship between the potential miRNAs and their target genes. Results miR‐144‐5p, found to be upregulated in all three studies, showed the greatest upregulation (P < 0.0001). Another 16 miRNAs (10 upregulated and six downregulated) overlapped between any two of the three studies. All overlapping miRNAs had expected expression levels except for miR‐203 during validation. Ten miRNAs (six upregulated and four downregulated) were found to have periodontal inflammation‐associated targets. Cyclooxygenase 2 (COX2) and interleukin‐17F (IL17F), predicted target genes of upregulated miR‐144‐5p, showed significant decreases and were negatively correlated with miR‐144‐5p in the periodontitis group (r = −0.742 for COX2, r = −0.615 for IL17F). Conclusion This re‐analysis of miRNA signatures has implied the potential regulatory mechanism of miR‐144‐5p and its potential for exploring alternative therapeutic approaches, especially those that use miRNA delivery systems to treat chronic periodontitis. Nevertheless, further study based on larger sample size and homogenous cells is needed to reveal the exact roles of miRNAs in chronic periodontitis.

show abstract

Section: Discussionmentioning

confidence: 99%

“…62,63 In addition, miR-204 may also be involved in the chondrogenic differentiation of periodontal ligament stem cells. 64 miR-513c was reported to inhibit the development of nervous system tumours and hepatocellular carcinoma. [65][66][67] Nevertheless, little research has been conducted to reveal their roles in periodontal inflammation.…”

Section: Discussionmentioning

confidence: 99%

Assessment of microRNA‐144‐5p and its putative targets in inflamed gingiva from chronic periodontitis patients

Wang

Chen

et al. 2018

J of Periodontal Research

View full text Add to dashboard Cite

show abstract

“…Osteoarthritis (OA), which is characterized by osteophyte formation, slight synovial inflammation, subchondral bone lesions, and destruction of articular cartilage, is the most common chronic joint disease. [1][2][3] The incidence of OA has been demonstrated to increase with age, especially in patients over the age of 65 years. [4][5][6] It is estimated that more than 100 million people are affected by OA worldwide.…”

Section: Introductionmentioning

confidence: 99%

Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis

et al. 2020

View full text Add to dashboard Cite

Osteoarthritis, a disorder characterized by articular cartilage deterioration, varying degrees of inflammation, and chondrocyte apoptosis, is the most common chronic joint disease. To slow or reverse its progression, inflammation should be inhibited, and chondrocyte proliferation should be promoted. Tetrahedral framework nucleic acids can be internalized by chondrocytes (even inflammatory chondrocytes) and can enhance their proliferation and migration. Wogonin, a naturally occurring flavonoid, suppresses oxidative stress and inhibits inflammation. In this study, tetrahedral framework nucleic acids were successfully selfassembled and used to load wogonin. We confirmed the effective formation of tetrahedral framework nucleic acid/wogonin complexes by dynamic light scattering, zeta potential analysis, transmission electron microscopy, and fluorescence spectrophotometry. Tetrahedral framework nucleic acids, wogonin, and especially tetrahedral framework nucleic acid/wogonin complexes effectively alleviated inflammation in vitro and in vivo and prevented cartilage destruction. In addition, these materials remarkably downregulated the expression of inflammatory mediators and matrix metalloproteinases, upregulated chondrogenic markers, and promoted tissue inhibitor of metalloproteinase 1 and B-cell lymphoma 2 expression. In vivo, after treatment with tetrahedral framework nucleic acid/wogonin complexes, the bone mineral density in regenerated tissues was much higher than that found in the untreated groups. Histologically, the complexes enhanced new tissue regeneration, significantly suppressed chondrocyte apoptosis, and promoted chondrogenic marker expression. They also inhibited cell apoptosis, increased chondrogenic marker expression, and suppressed the expression of inflammatory mediators in osteoarthritis. Therefore, we believe that tetrahedral framework nucleic acid/wogonin complexes can be used as an injectable form of therapy for osteoarthritis.Bone Research (2020) 8:6; https://doi.

show abstract

“…In addition to affecting osteogenesis and adipogenesis, the H3K27me3 demethylases KDM6A and KDM6B have also been reported to facilitate the chondrogenic differentiation of MSCs [84][85][86][87]. A study on Kdm6b −/− mice showed that KDM6B is recruited to the promoter of Runx2, a critical regulator of chondrocyte maturation and endochondral bone formation, and Ihh and activates the expression of these genes by decreasing the levels of repressive H3K27me3 marks [85].…”

Section: H3k27 Methylationmentioning

confidence: 99%

Control of mesenchymal stem cell biology by histone modifications

Ren

Huang

et al. 2020

Cell Biosci

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) are considered the most promising seed cells for regenerative medicine because of their considerable therapeutic properties and accessibility. Fine-tuning of cell biological processes, including differentiation and senescence, is essential for achievement of the expected regenerative efficacy. Researchers have recently made great advances in understanding the spatiotemporal gene expression dynamics that occur during osteogenic, adipogenic and chondrogenic differentiation of MSCs and the intrinsic and environmental factors that affect these processes. In this context, histone modifications have been intensively studied in recent years and have already been indicated to play significant and universal roles in MSC fate determination and differentiation. In this review, we summarize recent discoveries regarding the effects of histone modifications on MSC biology. Moreover, we also provide our insights and perspectives for future applications.

show abstract

KDM6A promotes chondrogenic differentiation of periodontal ligament stem cells by demethylation of SOX9

Cited by 44 publications

References 50 publications

Assessment of microRNA‐144‐5p and its putative targets in inflamed gingiva from chronic periodontitis patients

Assessment of microRNA‐144‐5p and its putative targets in inflamed gingiva from chronic periodontitis patients

Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis

Control of mesenchymal stem cell biology by histone modifications

Contact Info

Product

Resources

About