KDP, a Lactobacilli Product from Kimchi, Enhances Mucosal Immunity by Increasing Secretory IgA in Mice and Exhibits Antimicrobial Activity

Abstract

The potential of KDP, a lactic acid bacterial strain of Lactobacillus sakei, to enhance the production of mucosal specific immunoglobulin A (IgA) in mice and thereby enhance gut mucosal immunity was examined. KDP is composed of dead cells isolated from the Korean traditional food kimchi. Female BALB/c mice orally received 0.25 mg KDP once daily for 5 weeks and were co-administrated ovalbumin (OVA) for negative control and cholera toxin for positive control. Mice administered KDP exhibited increased secretory IgA (sIgA) contents in the small intestine, Peyer’s patches, serum, colon, and lungs as examined by ELISA. KDP also significantly increased the gene expression of Bcl-6, IL-10, IL-12p40, IL-21, and STAT4. In addition, KDP acted as a potent antioxidant, as indicated by its significant inhibitory effects in the range of 16.5–59.4% for DPPH, nitric oxide, maximum total antioxidant capacity, and maximum reducing power. Finally, KDP exhibited potent antimicrobial activity as evidenced by a significant decrease in the growth of 7 samples of gram-negative and gram-positive bacteria and Candida albicans. KDP’s adjuvant effect is shown to be comparable to that of cholera toxin. We conclude that KDP can significantly enhance the intestine’s secretory immunity to OVA, as well as act as a potent antioxidant and antimicrobial agent. These results suggest that orally administered KDP should be studied in clinical trials for antigen-specific IgA production.

Efficacy of lyophilized Lactobacillus sakei as a potential candidate for the prevention of carbapenem-resistant Klebsiella infection

“…In the in vivo experiment of this study, unlike the long-term oral treatment with L. sakei culture, the L. sakei powder showed a more signi cant effect in reducing the illness severity with an inhibitory effect on CRK strain because all infected mice survived until the end of this study. Other studies have also reported that the probiotic powder can prevent infections by several Gram-negative and Gram-positive pathogens and reduce antibiotic-resistant infections in in vivo experiments (Kechagia et al, 2013;Ghoneum and Abdulmalek, 2021).…”

Intestinal Flora: A Potential Mechanism by Which Yinlai Decoction Treats Lipopolysaccharide-Induced Pneumonia

“…The role of Bifidobacteria and Lactobacilli in maintaining human health and regulating immunity has been widely recognized. They are the most used intestinal probiotics [ 46 , 47 ]. As shown by our results, Yinlai decoction did not significantly increase the abundance of Bifidobacterium and Lactobacillus but did change the abundance of Leuconostocacea e in Lactobacillales , Aerococcaceae , Aerococcus , Carnobacteriaceae , and Trichococcus , of which the abundance of Leuconostocaceae significantly increased.…”

Identification of lactic acid bacteria with reduced inflammatory and enhanced protective properties against influenza virus

“…It is interesting to note the traditional methods that people have adopted in their daily diet to prevent viral infection. In addition, some LAB of the genus L. sakei reportedly activate mucosal immunity [39][40][41]. Thus, L. sakei appears to be highly beneficial for human health.…”