Ke 6 Gene

Abstract

Ke 6 gene is a newly identified gene located in the major histocompatibility complex and is a candidate steroid dehydrogenase gene because of structural homology and regulatory similarities with mammalian steroid dehydrogenases. We report here the complete nucleotide sequence and intron-exon organization of the Ke 6 gene and cloning of the alternatively spliced Ke 6b transcript. We find that Ke 6 gene expression is downregulated in pcy mice which is a murine model of polycystic kidney disease (PKD). Thus far, Ke 6 gene expression is down-regulated in all murine models of PKD we have examined. Abnormal steroid metabolism as a possible cause of PKD is discussed.

Physical Mapping 220 kb Centromeric of the Human MHC and DNA Sequence Analysis of the 43-kb Segment Including theRING1, HKE6,andHKE4Genes

“…The human HKE6 gene is composed of eight recognized within this Alu cluster by extensive homol-exons and seven introns with the same exon-intron ogy search or Grail I, IIa, or II analysis. organization as observed in the mouse Ke6 gene (Maxwell et al, 1995), and all of the intervening sequences The RING1 Gene have the consensus acceptor and donor splice sites. The exonic region in the human HKE6 gene shows 86.4% All of the five exons of the RING1 gene were recognized in this clone, and the complete genomic se-nucleotide identity with the mouse Ke6 exons (Maxwell et al, 1995).…”

“…organization as observed in the mouse Ke6 gene (Maxwell et al, 1995), and all of the intervening sequences The RING1 Gene have the consensus acceptor and donor splice sites. The exonic region in the human HKE6 gene shows 86.4% All of the five exons of the RING1 gene were recognized in this clone, and the complete genomic se-nucleotide identity with the mouse Ke6 exons (Maxwell et al, 1995). The nucleotide sequences between these quence of the RING1 gene was determined (Fig.…”

“…The and the human HKE6 cDNA (Ando et al, 1996) se-exon-intron organization was determined by compariquences, and the mouse Ke4 cDNA (St.-Jacques et al, son with the partial cDNA sequences of human and 1990) and human HKE4 cDNA (Ando et al, 1996) se-mouse as well as the mouse Ke6 genomic sequences quences. In this GC-rich region, no other coding se- (Ando et al, 1996;Maxwell et al, 1995) (Fig. 4).…”

“…The remaining proximal half of this region, 25 kb, mouse transcript (Ke6a) produced by alternative splichas a high AT content, and it contains a cluster of 31 ing from the Ke6 gene (Maxwell et al, 1995), has so Alu sequences, representing a density of 1.2 Alu per far been isolated from the HKE6 gene (Ando et al, kilobase; so far no coding sequence or exon has been 1996). The human HKE6 gene is composed of eight recognized within this Alu cluster by extensive homol-exons and seven introns with the same exon-intron ogy search or Grail I, IIa, or II analysis.…”

“…2. The highly homologous regions correspond to located 2.8 kb telomeric of the RING1 gene [between the human RING1 cDNA sequence (Lovering et al, the CAP site (the 5 end of the transcript) and the last 1993), the mouse Ke6 genomic (Maxwell et al, 1995) nucleotide of exon 9 of the HKE6 gene] (Fig. 2).…”