KEEFEKTIFAN BAKTERI ENDOFIT UNTUK MENGENDALIKAN NEMATODA Pratylenchus brachyurus PADA TANAMAN NILAM

HARNI, RITA; Supramana, Supramana; Sinaga, Meity Suradji; Giyanto, Giyanto; SUPRIADI, SUPRIADI

doi:10.21082/jlittri.v17n1.2011.6-10

Cited by 11 publications

(13 citation statements)

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“…The enzyme activity was observed by setting up two tubes; the first tube contained 5 ml of root extract and 5 ml of pyrogallol, and the second tube consisted of 5 ml of root extract, 5 ml of 0.5 M pyrogallol and 1% H 2 O 2 . Each tube was mixed for 5-10 s. The absorbance of each tube was measured at 420 nm using a spectrophotometer until the value become constant [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

Metabolomics Approach for The Analysis of Resistance of Four Tomato Genotypes (Solanum lycopersicum L.) to Root-Knot Nematodes (Meloidogyne incognita)

2019

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Metabolomics allows the identification of biochemical markers that have important roles in plant resistance to pests and diseases by which breeders can select plants based on differences in these compounds. This study examines the range of compounds associated with plant defense against nematodes. Resistant tomato genotypes, GM2 and F1 (GM2 × Hawai 7996), and susceptible genotypes, Gondol Putih and Gondol Hijau, were used in this study. Peroxidase activity was measured colorimetrically using a spectrophotometer. 1H-NMR (nuclear magnetic resonance) spectroscopy combined with orthogonal projections to latent structures discriminant analysis was used to analyze the metabolites involved in the tomato-nematode interactions. Identified signals were semi-quantitatively calculated by scaling the intensity of the 1H-NMR to the signals of an internal standard (trimethyl silyl-3-propionic acid) at 0.00 ppm. Resistant plants showed a higher peroxidase activity than susceptible plants. Chemical compounds that differentiated between susceptible and resistant plants were glucose and caffeic acid. Resistant tomatoes were observed to have seven times higher level of glucose than susceptible plants. Glucose is the primary metabolite that acts in the signaling pathways in plant defense mechanisms. Caffeic acid is one of the phenolic compounds alleged to have a negative effect on the nematode.

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Section: Methodsmentioning

confidence: 99%

Metabolomics Approach for The Analysis of Resistance of Four Tomato Genotypes (Solanum lycopersicum L.) to Root-Knot Nematodes (Meloidogyne incognita)

2019

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“…MUNIF (2001) menguji 181 isolat bakteri endofit asal tomat terhadap M. incognita, 21 isolat dapat menghambat perkembangan nematoda M. incognita di rumah kaca. Selanjutnya HARNI (2010) menggunakan isolat bakteri endofit asal nilam yaitu Achromobacter xylosoxidans, Bacillus subtilis, Alcaligenes faecalis, Bacillus cereus, dan Pseudomonas putida dapat menekan populasi Pratylenchus brachyurus 74,0-81,6% sekaligus dapat meningkatkan pertumbuhan nilam sebesar 46,97-86,79%, dan filtratnya dapat membunuh nematoda dalam waktu 24 jam dengan nilai LC 50 sebesar 7,71% (HARNI et al, 2010).…”

Section: Pendahuluanunclassified

POTENSI BAKTERI ENDOFIT MENGINDUKSI KETAHANAN TANAMAN LADA TERHADAP INFEKSI Meloidogyne incognita

Harni¹,

Ibrahim²

2020

j. penelitian tanam. industri

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ABSTRAKMeloidogyne incognita, merupakan salah satu organisme peng-ganggu (OPT) penyebab penyakit kuning pada tanaman lada dan dapatmengakibatkan penurunan hasil sampai 32%. Beberapa teknik untukmengendalikan patogen ini telah dilakukan tetapi belum memberikan hasilyang memuaskan. Pengendalian biologi dengan menggunakan bakteriendofit merupakan salah satu alternatif pengendalian yang cukup men-janjikan untuk dapat mengatasi permasalahan nematoda penyakit tanaman.Penelitian ini telah dilakukan di Laboratorium Bakteriologi danNematologi Departemen Proteksi Tanaman Institut Pertanian Bogor, danRumah Kaca Balai Penelitian Tanaman Rempah dan Aneka TanamanIndustri Pakuwon Sukabumi dari bulan Mei sampai November 2009.Kegiatan yang dilakukan adalah: 1) Seleksi beberapa isolat bakteri endofituntuk mengendalikan nematoda M. incognita pada tanaman lada dan 2)Potensi induced systemic resistance (ISR) dan analisis asam salisilat sertaperoksidase. Isolat bakteri endofit yang digunakan adalah isolat bakteriendofit potensial yang diisolasi dari akar nilam. Akar tanaman ladadirendam dalam suspensi bakteri endofit, selanjutnya diinokulasi dengan500 ekor larva 2 M. incognita. Sebulan setelah inokulasi tanamandibongkar diamati populasi nematoda dan pertumbuhan tanaman. AnalisisISR dilakukan dengan metode split root system dilanjutkan dengananalisis kadar asam salisilat dan peroksidase. Penelitian mengunakanRancangan Acak Lengkap. Hasil penelitian menunjukkan bahwa bakteriendofit dapat menekan jumlah puru dan populasi nematoda di dalam akar.Penekanan tertinggi pada isolat MSK (97,93%) tidak berbeda nyatadengan isolat BAS, TT2, dan NJ46 yaitu 97,35; 95,22; dan 92,14%.Berdasarkan analisis split root system, ke 4 isolat tersebut dapat meng-induksi ketahanan tanaman lada secara sistemik dengan mekanismepeningkatan kandungan asam salisilat dan peroksidase di dalam akar.Kata kunci : Bakteri endofit, penyakit kuning, Piper nigrum L.,Meloidogyne incognita, induksi ketahananABSTRACTThe use of endophytic bacteria to induce plant resistanceagainst infection of root-knot nematode (Meloidogyneincognita) on black pepperRoot-knot nematode (Meloidogyne incognita) is one of important patho-gens causing yellow disease on black pepper. As a result of this pathogenattack can lower the results up to 32%. Several control methods have beendone successful to control pathogen. Biological control using endophyticbacteria is one of prospective alternative control methods to overcomenematode problem. The research had been conducted in the Laboratory ofBacteriology and Nematology Department of Plant Protection, BogorAgricultural University (IPB) and in greenhouse of Indonesian Spices andIndustrial Crops Research Institute (ISICRI) Sukabumi. The objectives ofthis study were : 1) Selection of endophytic bacteria to control M.incognita nematodes on black pepper and 2) Potential of induced systemicresistance (ISR) and analysis of salicylic acid and peroxidase. Endophyticbacterial isolates used were endophytic potential bacterial isolates isolatedfrom the roots of patchouli. Pepper plant roots were soaked in anendophytic bacterial suspension, then inoculated with 500 larvae of 2 M.incognita. A month after inoculation, the plants were dismantled andobserved population of nematodes and plant growth. ISR analysis wasperformed by the method of split root system followed by analysis ofsalicylic acid and peroxidase contents. The research was arranged usingCompletely Randomized Design. The results showed that endophyticbacteria were able to suppress the amount of gall and nematode populationin roots. The highest suppression was on MSK isolate (97.93%) which wasnot significantly different from BAS, TT2, and NJ46 isolates, namely97.35, 95.22, and 92.14%, respectively. The analysis of split root systemshowed that the 4 isolates were able to induce systemic resistance of blackpepper with a mechanism of increase in salicylic acid and peroxidasecontents in roots.Key words : Endophytic bacteria, yellow disease, Piper nigrum L.,Meloidogyne incognita, induce systemic resistance

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“…Pengendalian biologi nematoda menggunakan bakteri endofit telah dilaporkan pada beberapa komoditas seperti kentang, pisang, kapas, padi dan beberapa tanaman hortikultura untuk mengendalikan nematoda puru akar (Meloidogyne incognita), nematoda ginjal (Rotylenchulus reniformis), nematoda kista (Globodera pallida), nematoda pelubang akar (Radopholus similis) dan nematoda peluka akar (Pratylenchus brachyurus) pada skala laboratorium, rumah kaca dan lapang (HALLMANN et al, 1997; SIKORA dan POCASANGRE, 2006;SIKORA 2007;HARNI et al, 2007).…”

Section: Pendahuluanunclassified

PENGARUH FILTRAT BAKTERI ENDOFIT TERHADAP MORTALITAS, PENETASAN TELUR DAN POPULASI NEMATODA PELUKA AKAR Pratylenchus brachyurus PADA NILAM

HARNI¹,

Supramana

Sinaga

et al. 2020

j. penelitian tanam. industri

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ABSTRAKPratylenchus brachyurus merupakan salah satu patogen utama padatanaman nilam di Indonesia. Pengendalian yang banyak dilakukan petanisaat ini adalah menggunakan pestisida sintetik. Penggunaan pestisidasintetik yang terus menerus merupakan ancaman terhadap lingkungan, dankesehatan manusia. Bakteri endofit mungkin dapat dimanfaatkan sebagaisalah satu teknik pengendalian nematoda yang ramah lingkungan karenabakteri endofit dapat menghasilkan racun yang toksik terhadap nematoda.Tujuan penelitian adalah melihat pengaruh kultur filtrat bakteri endofitterhadap mortalitas nematoda, penetasan telur dan perkembangannematoda di dalam akar nilam. Penelitian dilakukan di Laboratorium danRumah kaca Hama dan Penyakit Balai Penelitian Tanaman Obat danAromatik Bogor, dari bulan Januari sampai April 2008 menggunakanrancangan acak lengkap (RAL). Filtrat bakteri dibuat dengan caramenumbuhkan bakteri endofit pada media TSB selama 48 jam, kemudiandisentrifugasi dengan kecepatan 7.000 rpm selama 15 menit. Filtratdisaring dengan milipore berdiameter 0,22 µm, selanjutnya filtrat diujipada nematoda in vitro dan rumah kaca. Hasil penelitian menunjukkanbahwa filtrat dapat membunuh nematoda dalam waktu 24 jam dengannilai LC 50 sebesar 7,709%. Bakteri endofit isolat TT2 dan EH11memperlihatkan daya bunuh paling tinggi yaitu 91-100%. Di samping itufiltrat bakteri endofit juga dapat menekan penetasan telur nematoda 48,5-74,6% dibanding dengan kontrol. Namun hanya filtrat bakteri endofitisolat EH11 yang nyata dapat menekan populasi nematoda di dalam akarnilam dengan tingkat penekanan sebesar 81,3%.Kata kunci : Pratylenchus brachyurus, bakteri endofit, kultur filtrat,Pogostemon cablinABSTRACTEffect of culture filtrates endophytic bacteria on themortality, hatching eggs and population of root lesionnematodes Pratylenchus brachyurus on patchouliRoot lesion nematode (Pratylenchus brachyurus) is an importantpathogen of patchouli in Indonesia and causes significant losses. Controlsystem that are done today is using synthetic pesticides. The use ofsynthetic pesticides is a continuing threat to the environment and humanhealth. However, endophytic bacterial culture filtrates may be used as oneof the nematode control that is environmentally friendly. Effect of culturefiltrates endophytic bacteria on the mortality, hatching eggs and populationroot lesion nematodes Pratylenchus brachyurus on patchouli has beendone in vitro and greenhouse. The results showed that the culture filtrate ofendophytic bacteria produced metabolite toxic to nematodes and wereable to kill P. brachyurus 100% within 24 hours with LC 50 7.709%. TT2and EH11 isolates showed high killing power of 91-100%. The culturefiltrates also inhibited hatching of P. brachyurus eggs compared withcontrols. Not all culture filtrates can suppress the nematode population inthe roots of patchouli. EH11 isolates filtrate really pressing nematodepopulations compared to other isolates.Key words: Pratylenchus brachyurus, culture filtrate, endophyticbacteria, Pogostemon cablin

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KEEFEKTIFAN BAKTERI ENDOFIT UNTUK MENGENDALIKAN NEMATODA Pratylenchus brachyurus PADA TANAMAN NILAM

Cited by 11 publications

References 0 publications

Metabolomics Approach for The Analysis of Resistance of Four Tomato Genotypes (Solanum lycopersicum L.) to Root-Knot Nematodes (Meloidogyne incognita)

Metabolomics Approach for The Analysis of Resistance of Four Tomato Genotypes (Solanum lycopersicum L.) to Root-Knot Nematodes (Meloidogyne incognita)

POTENSI BAKTERI ENDOFIT MENGINDUKSI KETAHANAN TANAMAN LADA TERHADAP INFEKSI Meloidogyne incognita

PENGARUH FILTRAT BAKTERI ENDOFIT TERHADAP MORTALITAS, PENETASAN TELUR DAN POPULASI NEMATODA PELUKA AKAR Pratylenchus brachyurus PADA NILAM

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