Kefir treatment ameliorates dextran sulfate sodium-induced colitis in rats

Senol, Altug; Işler, Mehmet; Sütçü, Recep; Akın, Mete; Çakır, Ebru; Ceyhan, Betül Mermi; Koçkar, Muhammed Cem

doi:10.3748/wjg.v21.i46.13020

Cited by 27 publications

(17 citation statements)

References 56 publications

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“…Importantly, the DSS-induced colitis model is closer to human UC than it is to CD [37,47]. The most important pathophysiological changes in human UC are inflammatory in nature [4,5,7], which are readily reproduced in this model, as shown by the results of both the current study and others [40,47]. Oral administration of DSS is well-documented to induce an acute colitis model, with significant incidence of bloody diarrhea, colon mucosal damage, and granulocyte infiltration [38,48].…”

Section: Discussionsupporting

confidence: 59%

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Protective Effect of Spirulina platensis Extract against Dextran-Sulfate-Sodium-Induced Ulcerative Colitis in Rats

et al. 2019

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Inflammatory bowel disease is a multifactorial inflammatory condition. This study aimed to test the protective effects of Spirulina platensis against ulcerative colitis (UC). UC was induced in thirty-six male Wistar rats by adding dextran sulfate sodium (DSS) to their drinking water, while a control group received only drinking water. UC rats were equally-divided into six groups that received a single oral daily dose of vehicle (DSS), sulfasalazine (SSZ, 50 mg/kg/day), chloroform or the hydroalcoholic extracts of Spirulina platensis (100 or 200 mg/kg/day) for 15 days, and then blood and colon samples were harvested for determination of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), erythrocyte sedimentation rate (ESR), myeloperoxidase (MPO), and histopathology. At the end of the study, compared to time-matched controls, UC rats showed increased TNF-α (1.64-fold), IL-6 (5.73-fold), ESR (3.18-fold), and MPO (1.61-fold), along with loss of body weight (24.73%) and disease activity index (1.767 ± 0.216 vs. 0 ± 0), p < 0.001. These effects were prevented by SSZ treatment (p < 0.001 vs. DSS). The hydroalcoholic extract of Spirulina platensis dose-dependently modulated all DSS-induced inflammatory changes. However, the chloroform extract significantly lowered only IL-6 and ESR, but not TNF-α or MPO levels. The protective effects of the hydroalcoholic extract of Spirulina platensis against experimental UC involved mitigation of DSS-induced inflammation.

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Section: Discussionsupporting

confidence: 59%

“…The animals were monitored for changes in body weight on experimental days 1, 8, and 15. The DAI was calculated by taking the average of three parameter scores—the average body weight loss percentage, stool consistency, and the presence of fecal blood—every third day, according to previously reported methods [39,40].…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Spirulina platensis Extract against Dextran-Sulfate-Sodium-Induced Ulcerative Colitis in Rats

et al. 2019

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“…Inflammatory bowel disease is manifested by an inappropriate mucosal immune system response against intestinal luminal antigens (Farrell and LaMont, 2002;Sartor, 2004). Recently, Senol et al (2015) demonstrated that kefir reduces the clinical disease activity index and histological colitis scores in a dextran sulfate sodium-induced colitis model, possibly via reduction of myeloperoxidase (MPO), tumor necrosis factor-α (TNF-α), and inducible nitric oxide synthase levels. A relevant study also showed that Lactobacillus subspecies, L. salivarius 433118, and Bifido-8663 bacterium infantis 35624 were found to attenuate colitis in IL-10 knockout mice (McCarthy et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Therapeutic effects of kefir grain Lactobacillus-derived extracellular vesicles in mice with 2,4,6-trinitrobenzene sulfonic acid-induced inflammatory bowel disease

Seo¹,

Park²,

Ko³

et al. 2018

Journal of Dairy Science

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Kefir is a fermented product from yeast and lactic acid bacteria, and has been associated with various health benefits including relieving inflammatory bowel disease. Recently, it has been shown that gram-positive bacteria produce extracellular vesicles (EV). The EV could be appearing as potentially important mediators of cell to cell interaction. In this study, we explored the role of kefir grain Lactobacillus-derived EV in modulating inflammation responses via alleviating the production of inflammatory cytokines in tumor necrosis factor-α (TNF-α)-induced inflammation in Caco-2 cells and the 2,4,6-trinitrobenzene sulfonic acid-induced inflammatory bowel disease mouse model. Kefir-derived Lactobacillus EV were isolated by ultracentrifugation of the culture medium of 3 different kefir-derived strains (i.e., Lactobacillus kefir, Lactobacillus kefiranofaciens, and Lactobacillus kefirgranum). Nanoparticle tracking analysis showed that the size of isolated kefir-derived Lactobacillus EV was within 80 to 400 nm, and kefir-derived Lactobacillus EV uptake into recipient Caco-2 cells was confirmed by fluorescence labeling. Treatment of each kefir-derived Lactobacillus EV onto TNF-α-stimulated Caco-2 cells significantly reduced the level of both mRNA expression and secretion of IL-8, and Western blot analysis revealed that such an effect was related to inhibition of TNF-α signaling mediated by reducing the phosphorylation of p65, a subunit of NF-kB. Subsequent administration of kefir-derived Lactobacillus EV into inflammatory bowel disease-induced mice significantly alleviated the body weight loss and rectal bleeding, and enhanced stool consistency. Histological examination showed that kefir-derived Lactobacillus EV substantially reduced the infiltration of transmural leukocytes and loss of goblet cells within the colon, and the serum level of myeloperoxidase was significantly lower in the EV-treated group than control group. Our study demonstrates that kefir-derived Lactobacillus EV can be potentially used for developing innovative strategies for alleviating inflammatory bowel disease.

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“…Kefiran feeding has also been reported to cause increased levels of IgA+B cells, as well as IL‐6, IL‐10, and IL‐12 in the lamina propria of the small intestine in mice (Vinderola, Perdigón, Duarte, Farnworth, & Matar, ). A previous study conducted by some investigators from our group on the DSS‐induced colitis model, demonstrated that 5 ml kefir administration once a day reduced the disease activity index, histologic colitis scores, and the increase of TNF alpha (Senol et al, ).…”

Section: Discussionmentioning

confidence: 92%

“…An analysis of 25 kefir milk and associated grains sourced from eight geographically distinct regions using high‐through put sequencing showed that the bacterial populations in kefir were dominated by two phyla, the Firmicutes and the Proteobacteria (Marsh et al, ). Many previous studies have shown that kefir and kefir fractions have anti‐inflammatory and antioxidant activity (Bourrie et al, ), and the ameliorating effect of kefir on DSS‐induced colitis in rats was determined in one study (Senol et al, ).…”

Section: Introductionmentioning

confidence: 99%

Dose‐dependent effects of kefir on colitis induced by trinitrobenzene sulfonic acid in rats

Sevencan

Işler

Kapucuoğlu

et al. 2019

Food Science & Nutrition

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Evidence suggests that gut microbiota dysbiosis plays a critical role in the initiation and promotion of inflammatory bowel disease (IBD). Kefir is a fermented dairy product including yeast and bacterial species. We aimed to investigate the effect of kefir on trinitrobenzene sulfonic acid (TNBS)‐induced colitis in rats using two different doses. Fifty‐four Wistar rats were divided into six groups. For 14 days, the normal control and colitis control groups were given tap water, kefir10 control, kefir10 colitis, and kefir30 control, and the kefir30 colitis groups were given phosphate‐buffered saline containing 10% or 30% kefir, respectively, instead of tap water. Colitis was induced by intracolonically administrating TNBS in the colitis control, kefir10 colitis, and kefir30 colitis groups. On the 14th day, the rats were sacrificed. The weights and lengths of the colons were measured and macroscopically evaluated, and the distal 10 cm segments were subjected to a histopathological examination. The incidence of bloody stool and diarrhea in the kefir10 colitis group was found to be less than the colitis control and kefir30 colitis groups. The colonic weight/length ratio in the kefir10 colitis group was lower than that in the colitis control and kefir30 colitis groups. We detected that the 10% kefir treatment reduced TNBS‐induced macroscopic colonic damage, while it was exacerbated by the 30% kefir treatment. No significant difference was observed between the colitis groups in terms of microscopic colonic damage scoring. These results indicate that kefir, with a careful dose selection, may be a useful agent in the treatment of IBD.

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Kefir treatment ameliorates dextran sulfate sodium-induced colitis in rats

Abstract: Kefir reduces the clinical DAI and histologic colitis scores in a DSS-induced colitis model, possibly via reduction of MPO, TNF-α, and iNOS levels.

Cited by 27 publications

References 56 publications

Protective Effect of Spirulina platensis Extract against Dextran-Sulfate-Sodium-Induced Ulcerative Colitis in Rats

Protective Effect of Spirulina platensis Extract against Dextran-Sulfate-Sodium-Induced Ulcerative Colitis in Rats

Therapeutic effects of kefir grain Lactobacillus-derived extracellular vesicles in mice with 2,4,6-trinitrobenzene sulfonic acid-induced inflammatory bowel disease

Dose‐dependent effects of kefir on colitis induced by trinitrobenzene sulfonic acid in rats

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