Keishibukuryogan, a Traditional Japanese Medicine, Inhibits Platelet Aggregation in Guinea Pig Whole Blood

Abstract

Effects of keishibukuryogan (KBG) on platelet aggregation were investigated. To ensure the specificity of KBG, tokishakuyakusan (TSS) and kamisyoyosan (KSS), which are known to have platelet aggregation-inhibiting effects, and rikkunshito (RKT) and shakuyakukanzoto (SKT), which are considered to be devoid of such effects, were used for comparison. The platelet aggregation of each test drug was measured by the screen filtration pressure method using whole blood of guinea pigs and expressed as a collagen-induced pressure rate (%) or a collagen concentration required for 50% increase in the pressure rate (PATI value). KBG suppressed the collagen-induced whole blood pressure rate increase and increased the PATI value, like TSS and KSS. Neither RKT nor SKT showed these effects. The Moutan cortex and Cinnamomi cortex, the constituent crude drugs of KBG, showed KBG-like pressure rate suppression and PATI-increasing effects. Furthermore, paeonol, a representative component of Moutan cortex, and aspirin which is known to have platelet aggregation-inhibiting activity (COX-1 inhibitor) also showed similar effects. These results suggest that the platelet aggregation-inhibiting activity of the constituent crude drugs Moutan cortex and Cinnamomi cortex is involved in the improving effects of KBG on impaired microcirculation and that paeonol plays a role in these effects.

Antidepressant-Like Effects of Gyejibokryeong-hwan in a Mouse Model of Reserpine-Induced Depression

“…In the present study, we observed that GBH treatment significantly increased plasma serotonin levels while significantly decreasing corticosterone levels in reserpine-treated mice, relative to levels observed in control mice. Although plasma serotonin levels may represent release from both the central nervous system and platelets, GBH is known to suppress blood aggregation—a process that activates serotonin release [ 39 , 40 ]. Thus, it is unlikely that platelets represented the major source of serotonin release in our study.…”

Tokishakuyakusan, a traditional Japanese medicine (Kampo) mitigates iNKT cell‐mediated pregnancy loss in mice

“…19 The clinical usage of TSS is empirical, and the molecular mechanisms underlying the purported efficacy of TSS are limited. Regarding biological effects of TSS, vascular smooth muscle relaxation, 20 inhibition of platelet aggregation 21 and anti-inflammatory effect including induction of regulatory T cell 22,23 have been reported previously. This study is the first to report a potential mechanism to explain a documented improvement in pregnancy maintenance in mice upon dietary exposure to TSS.…”

GZFLW Induces Apoptosis of Ectopic Endometrial Stromal Cells via Promoting VPS53 Protein Stability

“…GZFLW, as a traditional Chinese medicine, owns many years of history for the treatment of endometriosis and has also been used for other diseases in animal models, such as dietary obesity in rats [23], improvement of microcirculation in mice [24], and inhibition of platelet aggregation in Guinea Pig [25]. However, due to the complexity of traditional Chinese medicine ingredients, its molecular biology mechanism is not yet clear.…”