KERAGAMAN GENETIK 24 VARIETAS PADI SAWAH DAN PADI GOGO (Oryza sativa L.) INDONESIA BERDASARKAN MARKA SSR

Abstract

A B S T R A C TThe use of molecular marker is an efficient approach to analyze the genetic diversity and it can be used widely in biological studies. The characterization of rice germplasms by using molecular markers technique is more precise because it is not influenced by environmental factors. The purpose of this study was to analyze the genetic diversity of 24 varieties of lowland and upland rice by using 15 SSR markers. The results showed as many as 86 alleles were detected in 24 rice varieties, with the average number of alleles per marker was 5.73 and the range of alleles per locus was 2-10. The average of major allele frequency was 43% with the lowest score was 26% on RM6997 and RM536 markers and the highest score was 65% on RM60 marker. A total of 14 SSR markers were able to discriminate heterozygous alleles within a range between 0.17 (RM105) to 1.00 (RM201, RM263, RM416, RM518 and RM223). The value of gene diversity ranged from 0.48 (RM60) to 0.81 (RM536) with an average of 0.70. The value of PIC (Polymorphic Information Content) ranged from 0.38 (RM105) to 0.78 (RM536) with an average of 0.65. The phylogenetic analysis showed that 24 rice varieties separate into two main clusters in the coefficient of 0.63. The first cluster consists of 12 lowland varieties and the second cluster consists of 12 upland varieties. The genetic diversity data in this study were expected could be a valuable information in the rice plant breeding activities in the future.

Review: The impact of social and economic change on domesticated plant diversity with special reference to wet rice field and home-garden farming of West Java, Indonesia

“…In many parts of Indonesia, most cultivated land is used for growing low-land wet rice (cf. Pelzer 1948;Gertz 1963;Puspita et al 2005;Sastrapradja and Widjaja 2010;Nugroho et al 2017). Wet rice farming is a common practice in this region as not only does it provide food income but also social status.…”

GENETIC DISTANCE OF 47 ACCESSIONS OF OIL PALM (Elaeis guineensis Jacq.) GERMPLASM FROM CAMEROON BASED ON MORPHOLOGICAL CHARACTER

“…Karakterisasi dan evaluasi plasma nutfah merupakan hal yang sangat penting dalam pemuliaan (Nugroho et al, 2017). Pada tanaman kelapa sawit, kegiatan karakterisasi tidak hanya mengidentifikasi jenis atau varietas kelapa sawit, tetapi juga menentukan jarak genetik di antara aksesi kelapa sawit tersebut.…”

“…Genotipe-genotipe yang mempunyai jarak genetik dekat tidak potensial untuk dijadikan bahan persilangan karena akan memperbesar peluang terjadinya inbreeding (Nugroho et al, 2017). Semakin jauh jarak genetik antar tetua maka peluang untuk menghasilkan kultivar baru dengan variabilitas genetik luas akan menjadi semakin besar (Miswarti et al, 2014).…”

SSR diversity on rice landraces collected from Yogyakarta Province

“…Microsatellite markers have been widely used to conduct various studies on rice genetic resources, for example, to study genetic diversity [8,[10][11][12][13][14][15][16], population structure [8], as well as association and mapping studies related to certain important characters [17][18][19][20][21][22].…”