Keratan sulphate is a marker of differentiation of ramified microglia

Bertolotto, Antonio; Manzardo, E.; Iudicello, Marco; Guglielmone, R; Riccio, A.

doi:10.1016/0165-3806(95)00030-h

Cited by 19 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 5D4‐positive cells were generally believed to be resting microglial cells (Bertolotto et al, 1995; Jander and Stoll, 1996), but our study showed that this population of microglia also phagocytosed DiI in the degenerating ganglion cells at 1 week and 1 month after axotomy. Furthermore, they appeared activated and performed phagocytosis without loss of their ramified morphologic characteristics.…”

Section: Discussioncontrasting

confidence: 57%

“…Bertolotto et al (1993, 1995) found that the 5D4 monoclonal antibody recognized an epitope of keratan sulfate proteoglycan (KSPG) and selectively stained ramified microglial cells, but not astrocytes, oligodendricytes, or ameboid microglia. The 5D4‐positive cells were present throughout the CNS parenchyma, but their numbers were fewer than those of cells labeled with the OX‐42 antibody or with isolectin B4, suggesting the 5D4 epitope may represent a subset of microglia.…”

Section: Discussionmentioning

confidence: 99%

“…It is believed that CD11b (or OX‐42 in rats) antibody is a general marker for microglia/macrophage cells. The 5D4 antibody is a recently identified marker for a subset of resting ramified microglia (Bertolotto et al, 1995). The distribution of 5D4‐positive cells and their response after optic axotomy have not previously been investigated.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Characterization of activated retinal microglia following optic axotomy

Zhang

Tso

2003

J of Neuroscience Research

View full text Add to dashboard Cite

Microglia are prominently involved in neural degenerative diseases of the CNS and the retina. In this study, we determined the activation and phagocytotic function of different subtypes of retinal microglial cells at 1 week and 1 month following optic axotomy. Fluorescent DiI crystals were placed at the stumps of the cut optic nerves of Lewis rats to retrolabel retinal ganglion cells. Microglial cells were indirectly labeled as they phagocytosed the dye particles in the dying ganglion cells. OX-42, 5D4, ED1, and OX-6 antibodies were used for immunohistochemical study. The OX-42- and 5D4-positive microglial cells were increased in the inner retinal layers after optic axotomy. The increase of OX-42-positive cells was considerably greater than that of 5D4-positive cells. The 5D4-positive cells were ramified in shape, whereas OX-42-positive cells were ameboid and ovoid. Both 5D4- and OX-42-positive cells phagocytosed dying ganglion cells at 1 week and 1 month after axotomy. Scattered ameboid ED1-positive cells were detected in the normal retina and showed phagocytotic activity at 1 month after optic axotomy. The number of ED1-positive cells in the retina was unchanged after axotomy. In optic axotomy, three types of microglial cells were activated, namely, 5D4-positive ramified cells and OX-42- and ED1-positive ameboid cells. All of them exhibited the phagocytosis of dying ganglion cells. Insofar as the blood-retinal barrier presumably remained intact in optic axotomy, the OX-42- and 5D4-positive cells might derive from resident microglial cells. The ED1-positive cells, presumably recently blood-borne macrophage in the CNS, remained the same number in the axotomized retina.

show abstract

Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of activated retinal microglia following optic axotomy

Zhang

Tso

2003

J of Neuroscience Research

View full text Add to dashboard Cite

show abstract

“…They thus propose that it may act not only to guide the developing neurites, but also to help maintain the adult structure. In the adult, KS is also a good marker for differentiated ramified microglia (Bertolotto et al, 1995).…”

Section: Are Ks and Pna Binding Sites Neuritic Guidance Molecules?mentioning

confidence: 99%

Keratan sulphate is present in developing chick skin in vivo where it could constitute a barrier to advancing neurites as observed in vitro

Hemming

Saxod

1997

J. Neurosci. Res.

View full text Add to dashboard Cite

“…(1989); phosphatidylinositol‐specific phospholipase C (PIPLC) pretreatment was performed according to Carey et al . (1990); heparinase I and chondroitinase ABC pretreatment was performed according to van Putten & Paul (1995); keratanase pretreatment was performed according to Bertolotto et al . (1995).…”

Section: Methodsmentioning

confidence: 99%

Evidence that glypican is a receptor mediating β-amyloid neurotoxicity in PC12 cells

Schulz

Megow

Reszka

et al. 1998

European Journal of Neuroscience

View full text Add to dashboard Cite

Docking of beta-amyloid fibrils to neuronal or glial cell membranes may be an early, necessary and intervenable step during the progression of Alzheimer's disease. Formation of neurofibrillary tangles and amyloid plaques as well as neurotoxicity and inflammation may be direct or indirect consequences. In an attempt to find a receptor that mediates those effects, we assessed rat pheochromocytoma PC12 cell 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) reduction after addition of beta-amyloid to the culture medium. Presence of competitive substances in the medium, cell-surface treatment and specific block of cellular synthesis pathways helped to identify the heparan sulphate moiety of a glycosylphosphatidylinositol-anchored protein likely to represent glypican as a possible receptor mediating beta-amyloid neurotoxicity.

show abstract

Keratan sulphate is a marker of differentiation of ramified microglia

Cited by 19 publications

References 30 publications

Characterization of activated retinal microglia following optic axotomy

Characterization of activated retinal microglia following optic axotomy

Keratan sulphate is present in developing chick skin in vivo where it could constitute a barrier to advancing neurites as observed in vitro

Evidence that glypican is a receptor mediating β-amyloid neurotoxicity in PC12 cells

Contact Info

Product

Resources

About