Keratin 17 is expressed during the course of SLS‐induced irritant contact dermatitis, but unlike keratin 16, the degree of expression is unrelated to the density of dividing keratinocytes

Willis, Carolyn M.; Reiche, Louise; Wilkinson, J. D.

doi:10.1111/j.1600-0536.1998.tb05806.x

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…This supports earlier concept for a role of type I IFN in Pso, which, however, appeared to be less relevant than in LP (Eriksen et al, 2005). Common upregulated genes in AD, Pso, and LPincluded cytokeratins (CK5 and CK17) and some S100 proteins (A2, A7/psoriasin, and A9/calgranulin), and reflected common clinical features like lymphocytic infiltration and inflammatory acanthosis (Ichikawa et al, 1997;Willis et al, 1998;Eckert et al, 2004;Martinsson et al, 2005). Chemokines and their receptors play a crucial part in the recruitment of leukocytes into inflammatory sites.…”

Section: Discussionsupporting

confidence: 83%

Gene Expression Profiling of Lichen Planus Reflects CXCL9+-Mediated Inflammation and Distinguishes this Disease from Atopic Dermatitis and Psoriasis

Wenzel

Peters

Zahn

et al. 2008

Journal of Investigative Dermatology

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Here, we present data of a gene expression profiling approach to apply the diagnostic value and pathological significance of this method in different inflammatory skin diseases, using whole skin biopsies. Initially, SAGE was performed to identify frequent tags differentially expressed in various skin diseases. On the basis of these results, a new skin pathology-oriented PIQOR microarray was designed. Lichen planus (LP) was chosen as a model disease to evaluate this system. Controls included healthy skin, atopic dermatitis (AD), and psoriasis (Pso). Gene expression analyses using the topic-defined microarray followed by unclassified clustering was able to discriminate LP from AD and Pso. Genes significantly expressed in LP included type I IFN inducible genes and a specific chemokine expression pattern. The CXCR3 ligand, CXCL9, was the most significant marker for LP. In situ hybridization and immunohistochemistry confirmed the results and revealed that keratinocytes are type I IFN producers in LP skin lesions. Our results show that gene expression profiling using a skin-specific microarray is a reliable method to identify patients with LP in the chosen context and reflect recent models concerning the pathogenesis of this disease. Gene expression profiling might complement the diagnostic spectrum in dermatology and may provide new pathogenetic insights.

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Section: Discussionsupporting

confidence: 83%

Gene Expression Profiling of Lichen Planus Reflects CXCL9+-Mediated Inflammation and Distinguishes this Disease from Atopic Dermatitis and Psoriasis

Wenzel

Peters

Zahn

et al. 2008

Journal of Investigative Dermatology

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“…Although it is not known which keratin 6 isoform is recognized by the antikeratin 6 antibody used in the present study, our findings indicate that EGF induces specific keratin 6a expression in reconstructed epidermis. This may have strong implications for wound healing and hyperproliferative disorders as has already been suggested in a number of studies which show differential keratin 16 and 17 expression (e.g., in nonfollicular epidermal tumors, 28 basal cell carcinomas, 29 hypertrophic scars, 30 irritant contact dermatitis 31 and after X‐ray irradiation 32 ).…”

Section: Discussionmentioning

confidence: 79%

Epidermal growth factor and keratinocyte growth factor differentially regulate epidermal migration, growth, and differentiation

Gibbs

Pinto

Murli

et al. 2000

Wound Repair Regeneration

115

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Various growth factors such as epidermal growth factor and keratinocyte growth factor have been reported to promote wound closure and epidermal regeneration. In the present study epidermis reconstructed on de-epidermized dermis was used to investigate the effects of epidermal growth factor and keratinocyte growth factor on keratinocyte proliferation, migration and differentiation. Our results show that epidermal growth factor supplemented cultures share many of the features which are observed during regeneration of wounded epidermis: a thickening of the entire epidermis, an enhanced rate of proliferation and migration, and an increase in keratin 6, keratin 16, skin-derived antileukoproteinase, involucrin and transglutaminase 1 expression. The increase in transglutaminase 1 protein is accompanied by an increase in the amount of active transglutaminase 1 enzyme. Surprisingly no increase in keratin 17 is observed. Prolonging the culture period for more than two weeks results in rapid senescence and aging of the cultures. In contrast, keratinocyte growth factor supplemented cultures have a tissue architecture that is similar to healthy native epidermis and remains unchanged for at least 4 weeks of air-exposure. The rate of proliferation and the expression of keratins 6, 16 and 17, skin-derived antileukoproteinase, involucrin and transglutaminase 1 is similar to that found in healthy epidermis and furthermore keratinocyte migration does not occur. When the culture medium is supplemented with a combination of keratinocyte growth factor and a low concentration of epidermal growth factor, skin-derived antileukoproteinase, involucrin and keratins 6, 16 and 17 expression is similar to that found in cultures supplemented with keratinocyte growth factor alone and in healthy epidermis. Only high transglutaminase 1 expression remains similar to that observed in cultures supplemented with epidermal growth factor alone. Our results show that the regulation of keratinocyte growth, migration and differentiation depends on the availability of these growth factors. Epidermal growth factor may play a dominant early role in wound healing by stimulating keratinocyte proliferation and migration while keratinocyte growth factor may play a role later in the repair process by stabilizing epidermal turnover and barrier function.

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“…CK17 reactivity can be observed in the hyperproliferating epidermis of psoriasis, but not in healthy skin (17,18), which correlates with our findings. A few studies have examined expression of CK17 in contact dermatitis (19), but to our knowledge there has been no comparison with psoriasis. In the current study, CK17 showed decreased expression in ACD compared with both psoriasis and EIP.…”

Section: Discussionmentioning

confidence: 99%

Eczema in Psoriatico: An Important Differential Diagnosis Between Chronic Allergic Contact Dermatitis and Psoriasis in Palmoplantar Localization

Kolesnik

Franke²,

Lux³

et al. 2018

Acta Derm Venerol

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Differential diagnosis of palmoplantar non-pustular psoriasis and chronic allergic contact dermatitis (ACD) and the combination of these conditions, termed "eczema in psoriatico" (EIP), is difficult, especially in cases of isolated involvement. A blind re-evaluation of 63 archived formalin-fixed palmoplantar samples, previously diagnosed clinically as either psoriasis or chronic ACD, was performed. Samples were allocated to histopathological diagnoses of psoriasis, contact dermatitis or EIP. Immunohistological stainings were performed for better characterization. Immunochemistry of EIP revealed features that overlapped contemporarily with psoriasis (cytokeratin 17 (CK17), Ki67, interleukin (IL)-8, IL-17, IL-23) and with ACD (CD1a, major histocompatibility complex (MHC) class I, MHC class II, epidermal T-cell subsets). Surprisingly, a significantly much higher number of dermal CD8+ T cells was found in EIP than in ACD and psoriasis. In conclusion, this study provides insight into the immunohistological differentiation of palmoplantar psoriasis, chronic ACD and EIP.

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Keratin 17 is expressed during the course of SLS‐induced irritant contact dermatitis, but unlike keratin 16, the degree of expression is unrelated to the density of dividing keratinocytes

Cited by 3 publications

References 19 publications

Gene Expression Profiling of Lichen Planus Reflects CXCL9+-Mediated Inflammation and Distinguishes this Disease from Atopic Dermatitis and Psoriasis

Gene Expression Profiling of Lichen Planus Reflects CXCL9+-Mediated Inflammation and Distinguishes this Disease from Atopic Dermatitis and Psoriasis

Epidermal growth factor and keratinocyte growth factor differentially regulate epidermal migration, growth, and differentiation

Eczema in Psoriatico: An Important Differential Diagnosis Between Chronic Allergic Contact Dermatitis and Psoriasis in Palmoplantar Localization

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