Keratin-associated protein micromaterials for medical and cosmetic applications

Lipkowski, Andrzej W.; Gajkowska, B; Grabowska, Anna; Kurzepa, Katarzyna

doi:10.14314/polimery.2009.386

Cited by 18 publications

(29 citation statements)

References 6 publications

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“…In this paper, we present for a second time a new method of obtaining natural hair-derived keratin-based wound dressing. 18 The big advantage of this method is the use of inexpensive and widely accessible raw material, inorganic reagents, utilization of an affordable enzyme such as pepsin, and lack of production of highly toxic or hard to neutralize wastes. Importantly, the insoluble keratin product obtained by this method does not affect cell viability and does not induce immunogenicity, either in an in vitro human cell model.…”

Section: Discussionmentioning

confidence: 99%

“…Keratin‐derived biomaterials studied so far, were prepared using chemical modifications and/or solubilization of keratins in biological materials, commonly derived from human, or animal origin (mouse or sheep) . Recently, we have proposed a new and inexpensive method to obtain insoluble, hair‐derived keratin‐based material which we used as a powder for wound dressing . In this study, we evaluated the utility of mouse fur‐derived keratin‐based dressing, that we named fur keratin‐derived protein (FKDP) and its influence on healing of a full‐thickness surgical skin wounds in allogenic mice.…”

mentioning

confidence: 99%

“…7 Recently, we have proposed a new and inexpensive method to obtain insoluble, hair-derived keratin-based material which we used as a powder for wound dressing. 18 In this study, we evaluated the utility of mouse fur-derived keratin-based dressing, that we named fur keratin-derived protein (FKDP) and its influence on healing of a full-thickness surgical skin wounds in allogenic mice.…”

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confidence: 99%

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The role of allogenic keratin‐derived dressing in wound healing in a mouse model

Konop

Sulejczak

Czuwara

et al. 2017

Wound Repair Regeneration

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Keratin is an interesting protein needed for wound healing and tissue recovery. We have recently proposed a new, simple and inexpensive method to obtain fur and hair keratin-derived biomaterials suitable for medical application. The aim of the study was to evaluate the role of the fur keratin-derived protein (FKDP) dressing in the allogenic full-thickness surgical skin wound model. The data obtained using scanning electron microscopy showed that employed processed biomaterial had higher surface porosity compared with control raw material. From the MTS test, it was found keratin biomaterial is not only toxic to the NIH/3T3 cell line (p < 0.05), but also enhances cell proliferation compared with the control. In vivo studies have shown keratin dressings are tissue biocompatible, accelerate wound closure and epithelialization to the statistically significant differences on day 5 (p < 0.05) in comparison to control wounds. Histological examination revealed, that in FKDP-treated wounds the inflammatory response contained predominantly macrophages whilst their morphological untreated variants showed mixed cell infiltrates rich in neutrophils. Predominant macrophages based response creates more favorable environment for healing. In FKDP-dressed wounds the number of microhemorrhages was also significantly decreased (p < 0.05) as compared with undressed wounds. Applied keratin dressing favors reconstruction of a more regular skin structure and assures better cosmetic effect in terms of scar formation and appearance. In conclusion, fur keratin-derived protein dressings significantly accelerated wound healing in the mouse model. Further studies are needed to determine the molecular mechanisms involved in the multilayer wound healing process and to assess the possible use of these dressings for medical purposes.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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The role of allogenic keratin‐derived dressing in wound healing in a mouse model

Konop

Sulejczak

Czuwara

et al. 2017

Wound Repair Regeneration

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“…Chicken feather, which is a biodegradable material, is abandoned in large amounts throughout the world every year, if the waste protein could be used as a valuable resource, it could not only turn waste to treasure, but also reduce environmental pollution. It has been reported in many studies in relation to the application of the wasted chicken feather . Bosco and his colleagues applied whey protein that acted as an agent to treat cotton fabric; they also assessed the effect of protein on the thermal and thermo‐oxidative stability and on flame retardant properties of the treated cotton.…”

Section: Introductionmentioning

confidence: 99%

Effect of chicken‐feather protein‐based flame retardant on flame retarding performance of cotton fabric

Wang

Chen

2014

J of Applied Polymer Sci

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A new kind of eco-friendly chicken-feather protein-based phosphorus-nitrogen-containing flame retardant was synthesized successfully with chicken-feather protein, melamine, sodium pyrophosphate, and glyoxal. And its structure was characterized by Fourier transform infrared spectroscopy, and the thermogravimetry of the agent was analyzed. Then the flame retarding performances of the chicken-feather protein-based flame retardant and in combination with the borax and boric acid in application to a woven cotton fabric were investigated by the vertical flammability test and limited oxygen index test. In addition, the surface morphologies of the treated and untreated fabrics were conducted by the scanning electron micrographs (SEM), and the thermogravimetric analyses of the treated and untreated cotton were explored, and the surface morphologies of char areas of the treated and untreated fabrics after burnt were tested by the SEM. The results showed that the flame retardancy of the cotton fabric treated by the chicken-feather protein-based flame retardant in combination with borax and boric acid was improved further, and the combination of the chickenfeather protein-based flame retardant and borax and boric acid could facilitate to form a homogenous and compact intumescing char layer, and the combination of them plays a good synergistic effect in the improvement of the flame retardancy of the treated cotton fabric.

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“…The dressings used for the experiments contained a commercially available lanolin ointment, 10% of keratin scaffolds prepared from alpaca and 1 mM one of the peptide analgesic hybrid compounds biphalin [M = 1,000 g/mol] or 3106 [M = 1,469 g/mol] in ointment (300 μL per dose). The wounds were created using the method described by Oriana et al with some modification.…”

Section: Methodsmentioning

confidence: 99%

The analgesic hybrid of dermorphin/substance P and analog of enkephalin improve wound healing in streptozotocin‐induced diabetic rats

Muchowska

Redkiewicz

Różycki

et al. 2019

Wound Repair Regeneration

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The purpose of this study was to investigate the effect of the peptide analgesic hybrid compounds: AWL3106 analog of dermorphin and substance P (7‐11), and biphalin enkephalin analog on wound healing in streptozotocin‐induced diabetic rats. The diabetes was induced in 6–7 week‐old male Wistar rats by intraperitoneal injection of streptozotocin. After 70 days, the wounds were created on the back of the rats and then, once a day for 21 days, the dressing containing lanolin ointment, 10% of keratin scaffolds, and 1 mM of AWL3106 or biphalin was applied. The wounds histology were analyzed by hematoxylin and eosin staining. The orientation and organization of collagen was analyzed by Masson's trichome staining. The number of macrophages, blood vessels, and fibroblasts were visualized by CD68, CD34, and vimentin immunoreactivity, respectively. Our results demonstrated that the wound area of AWL3106‐ and biphalin‐treated groups was greatly reduced (up to 47% on the 7 day) in comparison with untreated diabetic groups. The immunohistochemical staining of macrophages demonstrated that AWL3106 and biphalin accelerated inflammatory progression and subsequently decreased persistent inflammation. The histological analysis showed that the structure of tissue in the groups under the study was very similar to the one of wound tissue in N‐DM group. The H&E and Masson's trichome staining demonstrated that the orientation and organization of collagen as well as the number and shape of blood vessels were better in 3106‐ and BIF‐treated group than in DM group. In conclusion, the obtained data suggested that our hybrid peptides enhanced wound healing, particularly by accelerating the inflammatory phase and promoted the wound closure.

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Keratin-associated protein micromaterials for medical and cosmetic applications

Cited by 18 publications

References 6 publications

The role of allogenic keratin‐derived dressing in wound healing in a mouse model

The role of allogenic keratin‐derived dressing in wound healing in a mouse model

Effect of chicken‐feather protein‐based flame retardant on flame retarding performance of cotton fabric

The analgesic hybrid of dermorphin/substance P and analog of enkephalin improve wound healing in streptozotocin‐induced diabetic rats

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