Keratin Biomaterials Improve Functional Recovery in a Rat Spinal Cord Injury Model

Zabarsky, Zachary K; Dean, Gabriella M; Luo, T. David; Marquez-Lara, Alejandro; Jinnah, Alexander H.; Dyke, Mark Van; Smith, Thomas L.

doi:10.1097/brs.0000000000003993

Cited by 3 publications

(2 citation statements)

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“…Keratin/alginate scaffolds have been applied successfully for tissue regeneration in vitro [108]. Furthermore, keratin has been shown to promote Schwann cell proliferation in vitro and improve nerve regeneration in vivo [109,110] (Figures 3 and 4).…”

Section: Keratinmentioning

confidence: 99%

“…Promoted nerve regeneration and locomotor function [71] Abbreviations: PDGF-MS: platelet-derived growth factor-microsphere; MUSE-NPCs: neural progenitor cells differentiated in vitro from multilineage-differentiating stress-enduring cells. Improved functional recovery and inhibition of inflammatory response through macrophage polarization [110] Abbreviations: CSPG: chondroitin sulfate proteoglycans; VEGF: vascular endothelial growth factor; BDNF: brain-derived neurotrophic factor; MSCs: mesenchymal stem cells.…”

Section: Neural Stem Cells Spinal Cord Injurymentioning

confidence: 99%

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Bio-Scaffolds as Cell or Exosome Carriers for Nerve Injury Repair

Poongodi

Chen

Yang

et al. 2021

IJMS

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Central and peripheral nerve injuries can lead to permanent paralysis and organ dysfunction. In recent years, many cell and exosome implantation techniques have been developed in an attempt to restore function after nerve injury with promising but generally unsatisfactory clinical results. Clinical outcome may be enhanced by bio-scaffolds specifically fabricated to provide the appropriate three-dimensional (3D) conduit, growth-permissive substrate, and trophic factor support required for cell survival and regeneration. In rodents, these scaffolds have been shown to promote axonal regrowth and restore limb motor function following experimental spinal cord or sciatic nerve injury. Combining the appropriate cell/exosome and scaffold type may thus achieve tissue repair and regeneration with safety and efficacy sufficient for routine clinical application. In this review, we describe the efficacies of bio-scaffolds composed of various natural polysaccharides (alginate, chitin, chitosan, and hyaluronic acid), protein polymers (gelatin, collagen, silk fibroin, fibrin, and keratin), and self-assembling peptides for repair of nerve injury. In addition, we review the capacities of these constructs for supporting in vitro cell-adhesion, mechano-transduction, proliferation, and differentiation as well as the in vivo properties critical for a successful clinical outcome, including controlled degradation and re-absorption. Finally, we describe recent advances in 3D bio-printing for nerve regeneration.

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Section: Keratinmentioning

confidence: 99%

Section: Neural Stem Cells Spinal Cord Injurymentioning

confidence: 99%

Bio-Scaffolds as Cell or Exosome Carriers for Nerve Injury Repair

Poongodi

Chen

Yang

et al. 2021

IJMS

View full text Add to dashboard Cite

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Modifying Naturally Occurring, Nonmammalian-Sourced Biopolymers for Biomedical Applications

Shirk,

Heichel,

Eccles

et al. 2024

ACS Biomater. Sci. Eng.

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Natural biopolymers have a rich history, with many uses across the fields of healthcare and medicine, including formulations for wound dressings, surgical implants, tissue culture substrates, and drug delivery vehicles. Yet, synthetic-based materials have been more successful in translation due to precise control and regulation achievable during manufacturing. However, there is a renewed interest in natural biopolymers, which offer a diverse landscape of architecture, sustainable sourcing, functional groups, and properties that synthetic counterparts cannot fully replicate as processing and sourcing of these materials has improved. Proteins and polysaccharides derived from various sources (crustaceans, plants, insects, etc.) are highlighted in this review. We discuss the common types of polysaccharide and protein biopolymers used in healthcare and medicine, highlighting methods and strategies to alter structures and intra- and interchain interactions to engineer specific functions, products, or materials. We focus on biopolymers obtained from natural, nonmammalian sources, including silk fibroins, alginates, chitosans, chitins, mucins, keratins, and resilins, while discussing strategies to improve upon their innate properties and sourcing standardization to expand their clinical uses and relevance. Emphasis will be placed on methods that preserve the structural integrity and native biological functions of the biopolymers and their makers. We will conclude by discussing the untapped potential of new technologies to manipulate native biopolymers while controlling their secondary and tertiary structures, offering a perspective on advancing biopolymer utility in novel applications within biomedical engineering, advanced manufacturing, and tissue engineering.

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