Keratinase Production by Recalcitrant Feather Degrading Pseudomonas Geniculata and Its Plant Growth Promoting Activity

Abstract

We investigated the optimal conditions for keratinase production by feather-degrading Pseudomonas geniculata H10 using one variable at a time (OVT) method. The optimal medium composition and cultural condition for keratinase production were determined to be glucose 0.15% (w/v), beef extract 0.08% (w/v), KH2PO4 0.12% (w/v), K2HPO4 0.02% (w/v), NaCl 0.07% (w/v), MgSO4․7H2O 0.03%, MgCl2․6H2O 0.04% along with initial pH 10 at 200 rpm and 25℃, respectively. The production yield of keratinase was 31.6 U/ml in an optimal condition, showing 4.6-fold higher than that in basal medium. The strain H10 also showed plant growth promoting activities. This strain had ammonification activity and produced indoleacetic acid (IAA), siderophore and a variety of hydrolytic enzymes such as protease, lipase and chitinase. Therefore, this study showed that P. geniculata H10 could be not only used to upgrade the nutritional value of feather wastes but also useful in situ biodegradation of feather wastes. Moreover, it is also a potential candidate for the development of biofertilizing agent applicable to crop plant soil.

Biological synthesis and characterisation of silver nanoparticles using Pseudomonas geniculata H10 for pharmaceutical activity

“…P. geniculata H10 was isolated from human hair in our laboratory, as we previously described [22]. Cells were freshly inoculated into nutrient broth (peptone 1%, beef extract 1%, NaCl 0.5% and pH 6) and incubated at 200 rpm for 24 h at 30°C.…”

“…In a previous study, we isolated a novel Pseudomonas geniculata H10 from human hair and characterised feather degradation by this strain [22]. Interestingly, this strain produces various bioactive compounds, such as indoleacetic acid and siderophores and a variety of hydrolytic enzymes, including proteases, lipases, cellulases and chitinases, and this led us to investigate the SNPs biosynthesis by P. geniculata H10.…”