“…Cytoplasmic and nuclear proteins were extracted from the cells using NE-PER ™ Reagents (Thermo scientific, NY, United States). Total proteins, cytoplasmic proteins and nuclear proteins were then subjected to western blot performed as described in our previous study (Gao et al, 2021). The blot was incubated with primary antibody against caspase-3 (19677-1-AP, 1: 1,000, Proteintech, Wuhan, China), caspase-9 (66169-1-Ig, 1: 1,000, Proteintech, Wuhan, China), peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α) (66369-1-Ig, 1: 1,000, Proteintech, Wuhan, China), Nrf2 (66504-1-Ig, 1: 1,000, Proteintech,…”