Keratinocyte Monolayers on Hyaluronic Acid Membranes as “Upside-Down” Grafts Reconstitute Full-Thickness Wounds

Horch, Raymund E.; Wagner, Gilbert; Bannasch, Holger; Kengelbach‐Weigand, Annika; Arkudas, Andreas; Schmitz, Marweh

doi:10.12659/msm.915649

Cited by 8 publications

(5 citation statements)

References 30 publications

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“…Compared to the above methods, CST has significant advantages, which can resolve the above- mentioned drawbacks [ 45 , 46 ]. In the wound healing, mono-layer cell sheet or three-layer cell sheet is used to repair the wound healing [ 45 , 47 ]. Although a relatively high wound healing rate can be obtained, still a lot of improvement can be made.…”

Section: Resultsmentioning

confidence: 99%

Novel pre-vascularized tissue-engineered dermis based on stem cell sheet technique used for dermis-defect healing

Fan

Xie

Zhu

et al. 2020

Regenerative Biomaterials

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Insufficient donor dermis and the shortage of three-dimensional vascular networks are the main limitations in the tissue-engineered dermis (TED). To solve these problems, we initially constructed pre-vascularized bone marrow mesenchymal stem cell sheet (PBMCS) and pre-vascularized fibroblasts cell sheet (PFCS) by cell sheet technology, and then superimposed or folded them together to construct a pre-vascularized TED (PTED), aiming to mimic the real dermis structure. The constructed PTED was implanted in nude mice dorsal dermis-defect wound and the wound-healing effect was quantified at Days 1, 7 and 14 via the methods of histochemistry and immunohistochemistry. The results showed that PTED could rapidly promote the wound closure, especially at Day 14, and the wound-healing rate of three-layer PTED could reach 97.2% (P < 0.01), which was faster than the blank control group (89.1%), PBMCS (92.4%), PFCS (93.8%) and six-layer PTED (92.3%). In addition, the vessel density in the PTED group was higher than the other groups on the 14th day. Taken together, it is proved that the PTED, especially three-layer PTED, is more conducive to the full-thickness dermis-defect repair and the construction of the three-dimensional vascular networks, indicating its potential application in dermis-defect repair.

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Section: Resultsmentioning

confidence: 99%

Novel pre-vascularized tissue-engineered dermis based on stem cell sheet technique used for dermis-defect healing

Fan

Xie

Zhu

et al. 2020

Regenerative Biomaterials

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“…In 2019, Horch et al 40 studied in vivo the use of keratinocyte monolayers in hyaluronic acid membranes demonstrating that when keratinocytes were directly implanted towards the full-thickness wound bed of athymic mice, formation of a multilayered and differentiating epidermis was faster (14 days) than conventional technique (>21 days).…”

Section: Human Adult Skin Cells In Tesssmentioning

confidence: 99%

Cellular human tissue-engineered skin substitutes investigated for deep and difficult to heal injuries

et al. 2021

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Wound healing is an important function of skin; however, after significant skin injury (burns) or in certain dermatological pathologies (chronic wounds), this important process can be deregulated or lost, resulting in severe complications. To avoid these, studies have focused on developing tissue-engineered skin substitutes (TESSs), which attempt to replace and regenerate the damaged skin. Autologous cultured epithelial substitutes (CESs) constituted of keratinocytes, allogeneic cultured dermal substitutes (CDSs) composed of biomaterials and fibroblasts and autologous composite skin substitutes (CSSs) comprised of biomaterials, keratinocytes and fibroblasts, have been the most studied clinical TESSs, reporting positive results for different pathological conditions. However, researchers’ purpose is to develop TESSs that resemble in a better way the human skin and its wound healing process. For this reason, they have also evaluated at preclinical level the incorporation of other human cell types such as melanocytes, Merkel and Langerhans cells, skin stem cells (SSCs), induced pluripotent stem cells (iPSCs) or mesenchymal stem cells (MSCs). Among these, MSCs have been also reported in clinical studies with hopeful results. Future perspectives in the field of human-TESSs are focused on improving in vivo animal models, incorporating immune cells, designing specific niches inside the biomaterials to increase stem cell potential and developing three-dimensional bioprinting strategies, with the final purpose of increasing patient’s health care. In this review we summarize the use of different human cell populations for preclinical and clinical TESSs under research, remarking their strengths and limitations and discuss the future perspectives, which could be useful for wound healing purposes.

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“…There have been numerous reports on these acellular skin substitutes being used as scaffolds in conjunction with the sheet form of CEA for complex wounds. 7,[12][13][14][15] Matsumura et al 16 reported the successful use of combined CEA and acellular skin substitution in severe burn patients.…”

Section: Original Article Smjmentioning

confidence: 99%

Keratinocyte Culture: Siriraj’s Experience

et al. 2022

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Objective: Cell-based therapy is gaining increasing prominence in medicine, where it has the potential to replace or repair damaged tissue using new engineered cells. Skin cell engineering, also known as keratinocyte culture or cultured epithelial autograft (CEA), is a promising field in cell-based therapy. CEA is now used in many parts of the world as an alternative treatment for some diseases that require large defects to be covered, such as severe and major burn patients and congenital melanocytic nevus. The use of CEA in conjunction with acellular skin substitution is rapidly expanding.Materials and Methods: This study is an initiative aimed at supporting the production and use of keratinocyte cultures at Siriraj Hospital. This is the first stage of developing sheet keratinocyte culture in vitro.Results: Our study yielded very promising results. As feeder cells, we used irradiated 3T3 murine fibroblasts, as per the standard protocol for keratinocyte culture. The growth duration was four weeks: 2 weeks for the 3T3 murine fibroblasts and 2 weeks for the keratinocytes. The keratinocytes grew rapidly and formed sheets with irradiated 3T3 murine fibroblasts. The retrieval of the cell sheets was straightforward thanks to the temperature-response cell culture dish and halo-ring cell recovery sheet. Flow cytometry revealed that the cells had a very high viability and purity. H&E staining revealed the sheets comprised two to four layers of stratified epithelial tissue.Conclusion: From this study, our method of manufacturing the CEA can offer a promising result. This can be use in the treatment which require large skin coverage. However, we aim to initiate animal and human trial phase next.

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Keratinocyte Monolayers on Hyaluronic Acid Membranes as “Upside-Down” Grafts Reconstitute Full-Thickness Wounds

Cited by 8 publications

References 30 publications

Novel pre-vascularized tissue-engineered dermis based on stem cell sheet technique used for dermis-defect healing

Novel pre-vascularized tissue-engineered dermis based on stem cell sheet technique used for dermis-defect healing

Cellular human tissue-engineered skin substitutes investigated for deep and difficult to heal injuries

Keratinocyte Culture: Siriraj’s Experience

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