Keratinolytic activities of alkaliphilic Bacillus sp. MBRL 575 from a novel habitat, limestone deposit site in Manipur, India

Kshetri, Pintubala; Ningthoujam, Debananda S.

doi:10.1186/s40064-016-2239-9

Cited by 29 publications

(15 citation statements)

References 61 publications

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“…[45] and Streptomyces pactum [49]. The initial step in keratin degradation catalyze by disulfide reductase has also been reported by other researchers [50][51][52][53]. Reduction of disulfide bonds changes the conformation of amino acids in β-sheet of keratin rendering different hydrolytic sites for proteolytic attack by keratinase [16,54].…”

Section: Mechanism Of Feather Degradationmentioning

confidence: 61%

“…The strain was also positive for cell wall degrading enzymes such as pectinase, protease, and lipase [22]. Similarly, Kshetri and Ningthoujam [52] reported keratinolytic Bacillus sp. inhibiting the growth of Rhizoctonia solani and Rhizoctonia oryzae-sativa.…”

Section: Disulfide Reductase Protease Deaminationmentioning

confidence: 82%

“…Antifungal IAA production P solubilization Ammonia production [58] Bacillus sp. Antifungal [52] Bacillus sp. Antinematodal [59] Bacillus sphaericus Insecticidal [60] Bacillus subtilis Antifungal IAA production P solubilization Ammonia production [57] Bacillus thuringiensis Insecticidal [60] Chryseobacterium sediminis IAA production [72] Stenotrophomonas maltophilia Antifungal IAA production Ammonia production [22] Streptomyces sp.…”

Section: Biofertilizing Potentialmentioning

confidence: 99%

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Feather degradation by keratinolytic bacteria and biofertilizing potential for sustainable agricultural production

et al. 2018

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Feathers account for 5–7% of the total weight of chicken have become one of the major pollutants due to their recalcitrant nature. Feather which is constituted of 90% keratin can be a good source of peptides, amino acids, and minerals for use as organic fertilizer. Traditional feather degradation methods consume large amount of energy and reduces the overall quality of the proteins. However, degradation of keratin by keratinolytic bacteria may represent as an alternative for the development of cheap, cost effective, eco‐friendly, and easily available nitrogen (N) and minerals rich source as potential organic fertilizers. Keratinase enzymes from bacteria are serine‐type proteases showing optimal activity at pH 6 to 9 and 30 to 50 °C. Mechanism of degradation includes, sulfitolysis, proteolysis, followed by deamination. Keratinolytic bacteria showing antagonism against important plant pathogens may act as biocontrol agent. Feather hydrolyzate can also be employed as nitrogenous fertilizers for plant growth. Tryptophan release from the feather degradation can act as precursor for plant phytohormone, indole‐3‐acetic acid (IAA). Solubilization of inorganic phosphate (P) by keratinolytic bacteria may further elevate the growth of plant. Application of hydrolyzate increases the water holding capacity, N, carbon (C) and mineral content of the soil. It elevates protein, amino acids, and chlorophyll content of plant. Feather hydrolyzate enhances seed germination and growth of plant. Soil application further increases the population of beneficial bacteria. The use of keratinolytic bacteria having antagonistic and plant growth promoting activities, and feather hydrolyzate can emerge as sustainable and alternative tools to promote and improve organic farming, agro‐ecosystem, environment, human health, and soil biological activities.

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Section: Mechanism Of Feather Degradationmentioning

confidence: 61%

Section: Disulfide Reductase Protease Deaminationmentioning

confidence: 82%

Section: Biofertilizing Potentialmentioning

confidence: 99%

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Feather degradation by keratinolytic bacteria and biofertilizing potential for sustainable agricultural production

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“…FPF-1 is significantly higher than has been reported for Bacillus sp. MBRL 575 [34] and Stenotrophomonas maltophilia R13 [25]. Consequently, Bacillus sp.…”

Section: Time Course Profile Of the Keratinolytic Activity By Bacillumentioning

confidence: 99%

Bacillus sp. FPF-1 Produced Keratinase with High Potential for Chicken Feather Degradation

2020

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Chicken feathers are predominantly composed of keratin; hence, valorizing the wastes becomes an imperative. In view of this, we isolated keratinase-producing bacteria and identified them through the 16S rDNA sequence. The process condition for keratinase activity was optimized, and electron micrography of the degradation timelines was determined. Keratinolytic bacteria were isolated and identified as Bacillus sp. FPF-1, Chryseobacterium sp. FPF-8, Brevibacillus sp. Nnolim-K2, Brevibacillus sp. FPF-12 and Brevibacillus sp. FSS-1; and their respective nucleotide sequences were deposited in GenBank, with the accession numbers MG214993, MG214994, MG214995, MG214996 and MG214999. The degree of feather degradation and keratinase concentration among the isolates ranged from 62.5 ± 2.12 to 86.0 ± 1.41(%) and 214.55 ± 5.14 to 440.01 ± 20.57 (U/mL), respectively. In the same vein, 0.1% (w/v) xylose, 0.5% (w/v) chicken feather, an initial fermentation pH of 5.0, fermentation temperature of 25 °C and an agitation speed of 150 rpm, respectively, served as the optimal physicochemical conditions for keratinase activity by Bacillus sp. FPF-1. The time course showed that Bacillus sp. FPF-1 yielded a keratinase concentration of 1698.18 ± 53.99(U/mL) at 120 h. The electron microscopic imaging showed completely structural dismemberment of intact chicken feather. Bacillus sp. FPF-1 holds great potential in the valorization of recalcitrant keratinous biomass from the agro sector into useful products.

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“…Mehta et al (2014) studied the scanning electron microscopy of chicken feather degradation after seven days by Bacillus sonorensis at 80009 magnification. Kshetri and Ningthoujam (2016) reported the degradation of chicken and other bird feathers by Bacillus sp. MBRL 575.…”

Section: Discussionmentioning

confidence: 99%

Chrysosporium queenslandicum: a potent keratinophilic fungus for keratinous waste degradation

Kumawat¹,

Sharma²,

Bhadauria³

2017

Int J Recycl Org Waste Agricult

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Purpose Keratinous wastes are the solid environmental pollutant generated from poultry farms, slaughterhouses and barber's shops. The aim of the present study is the degradation of keratinous wastes in an eco-friendly way by biological methods, which should further be helpful to reduce the wastes and recycled into valuable feed and fertilizers. Methods Degradation of keratinous substrates was assessed by highly potent keratinophilic fungi, namely Chrysosporium queenslandicum TKKASb Apinis and R.G. Rees. This chicken feather degrading fungal strain previously isolated and identified by morphological and 18 s rDNA sequencing in laboratory, was used in the present study. Ch. queenslandicum was inoculated into the basal salt medium (BSM) with keratinous substrates for 12 and 24 days at 28 ± 2°C to observe degradation. The rate of degradation was expressed as weight loss of keratin substrate over incubation days and pH variation. Results In this study, Ch. queenslandicum showed maximum degradation on chicken feathers followed by human nail clippings, animal hair and human hair. The degradation rate on chicken feather was 38.40 ± 0.80 and 46.40 ± 2.50% after 12 and 24 days, respectively. The basal salt medium's pH was increased over incubation time. Scanning electron microscopy (SEM) examination also demonstrated the degradation of chicken feathers. Conclusions The results suggest that Ch. queenslandicum possess the potential biotechnological applications which can be used in the hydrolysis of keratinous waste and recycling of poultry waste for environmental protection. The hydrolyzed keratinous material can also be utilized as the source of fertilizers for plants and feed for animals.

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Keratinolytic activities of alkaliphilic Bacillus sp. MBRL 575 from a novel habitat, limestone deposit site in Manipur, India

Cited by 29 publications

References 61 publications

Feather degradation by keratinolytic bacteria and biofertilizing potential for sustainable agricultural production

Feather degradation by keratinolytic bacteria and biofertilizing potential for sustainable agricultural production

Bacillus sp. FPF-1 Produced Keratinase with High Potential for Chicken Feather Degradation

Chrysosporium queenslandicum: a potent keratinophilic fungus for keratinous waste degradation

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