Kerosene tolerance in Achromobacter and Pseudomonas species

Abstract

The aim of the present study was to investigate the tolerance of five new Achromobacter and Pseudomonas strains to kerosene and to establish if the production of several secondary metabolites increases or not when these bacteria were grown in the presence of kerosene. The biodegradation of kerosene by isolated bacteria was also investigated in this study. Methods: Five Proteobacteria were isolated from different samples polluted with petroleum and petroleum products. Based on their morphological, biochemical, and molecular characteristics, isolated bacteria were identified as Achromobacter spanius IBB Po18 and IBB Po21 , Pseudomonas putida IBB Po19 , and Pseudomonas aeruginosa IBB Po20 and IBB Po22. Results: All these bacteria were able to tolerate and degrade kerosene. Higher tolerance to kerosene and degradation rates were observed for P. aeruginosa IBB Po20 and IBB Po22 , compared with that observed for A. spanius IBB Po18 and IBB Po21 , and P. putida IBB Po19. All these bacteria were able to produce several secondary metabolites, such as surfactants and pigments. Glycolipid surfactants produced by P. aeruginosa IBB Po20 and IBB Po22 , A. spanius IBB Po18 and IBB Po21 , and P. putida IBB Po19 have a very good emulsification activity, and their activity increased when they were grown in the presence of kerosene. The production of rhamnolipid surfactants by P. aeruginosa IBB Po20 and IBB Po22 was confirmed by detection of rhlAB gene involved in their biosynthesis. Pyocyanin and pyoverdin pigments were produced only by P. aeruginosa IBB Po20 and IBB Po22 , while carotenoid pigments were produced by all the isolated bacteria. Significant changes in pigments production were observed when P. aeruginosa IBB Po20 and IBB Po22 , A. spanius IBB Po18 and IBB Po21 , and P. putida IBB Po19 were grown in the presence of kerosene. Conclusion: Due to their ability to tolerate and degrade kerosene, and also to produce several secondary metabolites, the isolated bacteria could be used in the bioremediation of kerosene-polluted environments.

Tyrosinase Enzyme Purification and Immobilization from Pseudomonas sp. EG22 Using Cellulose Coated Magnetic Nanoparticles: Characterization of Bioactivity in Melanin Product

“…Secondly, the immobilized enzyme had enhanced access to the substrate, resulting in a higher reaction rate. Finally, the immobilized enzyme was less susceptible to inhibition by inhibitors present in the culture medium (Huang et al 2012;Brena et al 2013;Stancu 2020).…”

Kerosene Biodegradation by Highly Efficient Indigenous Bacteria Isolated From Hydrocarbon-Contaminated Sites

“…1 A large group of bacterial species (Gram-positive and Gram-negative) has been identified as potential kerosene degraders, including Pseudomonas spp., Bacillus spp., Acinetobacter spp., Escherichia spp., Proteus spp., Serratia spp., Streptomyces spp., Arthrobacter spp., Gordonia spp., Brevibacerium spp., Burkholderia spp., Mycobacterium spp., Nocardia spp., Xanthomonas spp., Alcaligenes spp., Flavobacterium spp., Micrococcus spp., and Corneybacterium spp. 13,[16][17][18] Effective biodegradation is influenced by biological characteristics and environmental parameters that exist within polluted sites. 19 These include the physicochemical nature of hydrocarbon pollutants, 20 microbial population quality (size, single strain, and consortia), 21 presence of xenobiotic pollutants such as heavy metals, 1 and environmental parameters in polluted sites such as nutrients availability, range of temperature and pH, soil moisture, and oxygen.…”

“… 1 A large group of bacterial species (Gram-positive and Gram-negative) has been identified as potential kerosene degraders, including Pseudomonas spp., Bacillus spp., Acinetobacter spp., Escherichia spp., Proteus spp., Serratia spp., Streptomyces spp., Arthrobacter spp., Gordonia spp., Brevibacerium spp., Burkholderia spp., Mycobacterium spp., Nocardia spp., Xanthomonas spp., Alcaligenes spp., Flavobacterium spp., Micrococcus spp., and Corneybacterium spp. 13 , 16 - 18 …”

The study on the possibility of using sewage sludge in the ecological reconstruction of contaminated sites from the petroleum industry

“…To determine the number of heterotrophic, hydrocarbon-tolerant, hydrocarbon-degrading bacteria, and enterobacteria that exist in the analyzed samples the plate culture method was used [7]. From the samples, serial dilutions (10 -1 -10 -6 ) were made in phosphate saline buffer, which were then inoculated in Petri dishes with solid LB nutrient medium [10], to determine the total number of heterotrophic bacteria, solid LB nutrient medium supplemented with diesel fuel (5% v/v) for determining the number of hydrocarbon-tolerant bacteria, solid mineral medium [11] supplemented with diesel fuel (5% v/v) for determining the number of hydrocarbon-degrading bacteria, and solid EMB medium for determining the total number of enterobacteria. For each dilution, 2 repetitions were done.…”

“…It could be observed that over time the petroleum products that reached the basement underwent a degradation process due to the consortia of bacteria that metabolize petroleum hydrocarbons [6]. The spilling of petroleum and petroleum products into the soil results in a numerical increase in bacterial populations that use hydrocarbons as a carbon source, with a concomitant reduction in bacterial diversity and the predominance of species that tolerate and/or degrade hydrocarbons [7][8][9][10][11].…”