Ketamine and the Myenteric Plexus in Intestinal Ischemia/Reperfusion Injury

Garza, Francisco Javier Guzmán-de la; Cámara-Lemarroy, Carlos R.; Ballesteros-Elizondo, Raquel Guadalupe; Alarcón-Galván, Gabriela; Cordero-Pérez, Paula; Fernández-Garza, Nancy Esthela

doi:10.1007/s10620-009-0976-0

Cited by 11 publications

(6 citation statements)

References 35 publications

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“…Such change has been hypothesized to be, at least in part, at the basis of development of visceral hypersensitivity during colonic inflammation. As concerns the intestinal ischemic/reperfusion injury, recent reports have demonstrated that NMDA glutamatergic pathways are involved in the functional changes underlying gastrointestinal dismotility in these conditions 18,49,50 . In the guinea pig ileum, one of the functional consequences of the potentiation of the glutamatergic transmission after glucose/oxygen deprivation is represented by an anomalous increase in the overflow of acetylcholine 19 .…”

Section: Discussionmentioning

confidence: 99%

Protein kinase c modulates NMDA receptors in the myenteric plexus of the guinea pig ileum during in vitro ischemia and reperfusion

Giaroni

Zanetti

Giuliani

et al. 2010

Neurogastroenterology &Amp; Motility

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Section: Discussionmentioning

confidence: 99%

Protein kinase c modulates NMDA receptors in the myenteric plexus of the guinea pig ileum during in vitro ischemia and reperfusion

Giaroni

Zanetti

Giuliani

et al. 2010

Neurogastroenterology &Amp; Motility

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“…However, D-lactate was not elevated in the hyperacute phase of intestinal ischemia (1 hour of reperfusion). The specific roles of other markers, such as iFABP, coagulation parameters (41), oxidative stress (42), and adhesion molecules (40), requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Temporal relationship of serum markers and tissue damage during acute intestinal ischemia/reperfusion

Garza¹,

Ibarra-Hernández²,

Cordero-Pérez³

et al. 2013

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OBJECTIVE:It is essential to identify a serological marker of injury in order to study the pathophysiology of intestinal ischemia reperfusion. In this work, we studied the evolution of several serological markers after intestinal ischemia reperfusion injury in rats. The markers of non-specific cell damage were aspartate aminotransferase, alanine aminotransaminase, and lactic dehydrogenase, the markers of inflammation were tumor necrosis factor alpha, interleukin-6, and interleukin-1 beta, and the markers of intestinal mucosal damage were intestinal fatty acid binding protein and D-lactate. We used Chiús classification to grade the histopathological damage.METHODS:We studied 35 Wistar rats divided into groups according to reperfusion time. The superior mesenteric artery was clamped for 30 minutes, and blood and biopsies were collected at 1, 3, 6, 12, 24, and 48 hours after reperfusion. We plotted the mean ± standard deviation and compared the baseline and maximum values for each marker using Student's t-test.RESULTS:The maximum values of interleukin-1 beta and lactic dehydrogenase were present before the maximal histopathological damage. The maximum tumor necrosis factor alpha and D-lactate expressions coincided with histopathological damage. Alanine aminotransaminase and aspartate aminotransferase had a maximum expression level that increased following the histopathological damage. The maximum expressions of interluken-6 and intestinal fatty acid binding protein were not significantly different from the Sham treated group.CONCLUSION:For the evaluation of injury secondary to acute intestinal ischemia reperfusion with a 30 minute ischemia period, we recommend performing histopathological grading, quantification of D-lactate, which is synthesized by intestinal bacteria and is considered an indicator of mucosal injury, and quantification of tumor necrosis factor alpha as indicators of acute inflammation three hours after reperfusion.

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“…Additionally, a single dose of 50 mg/kg ketamine was administered intraperitoneally to this group after the termination of priapism and the application of constriction bands. [ 8 ] At the end of the experiment, penectomy was performed for histopathological examination, and blood samples were collected from the inferior vena cava for biochemical analysis. After all procedures were completed, rats were euthanized by cervical dislocation.…”

Section: Methodsmentioning

confidence: 99%

Effects of ketamine on penile tissues in an experimental priapism model in rats

Kölükçü

2024

Ulus Travma Acil Cerrahi Derg

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BACKGROUND: This study aimed to evaluate the histopathological and biochemical effects of ketamine on penile tissues following ischemia-reperfusion injury induced by priapism. METHODS: Twenty-four male rats were randomized into three groups. Group 1 served as the control group. Group 2 underwent the priapism model to induce ischemia-reperfusion injury. Group 3, the treatment group, experienced a similar ischemia-reperfusion model as Group 2; additionally, 50 mg/kg of ketamine was administered intraperitoneally just before reperfusion. Blood biochemical analyses and penile histopathological evaluations were performed. RESULTS: In Group 3, significant improvements were observed in all histopathological scores, including desquamation, edema, inflammation, and vasocongestion compared to Group 2 (p<0.001). Blood biochemical analyses showed that the malondialdehyde (MDA) levels were recorded as 10 in Group 2, with a significant decrease in Group 3 (p=0.013). Similarly, proinflammatory cytokine levels, including interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), were found to be suppressed in Group 3 compared to Group 2 (p=0.003, p=0.022, and p=0.028, respectively). Antioxidant enzyme activities, such as glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), were higher in Group 3 compared to Group 2 (p=0.016 and p=0.024, respectively). CONCLUSION: Ketamine is an effective anesthetic agent in alleviating the effects of penile ischemia-reperfusion injury.

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Ketamine and the Myenteric Plexus in Intestinal Ischemia/Reperfusion Injury

Cited by 11 publications

References 35 publications

Protein kinase c modulates NMDA receptors in the myenteric plexus of the guinea pig ileum during in vitro ischemia and reperfusion

Protein kinase c modulates NMDA receptors in the myenteric plexus of the guinea pig ileum during in vitro ischemia and reperfusion

Temporal relationship of serum markers and tissue damage during acute intestinal ischemia/reperfusion

Effects of ketamine on penile tissues in an experimental priapism model in rats

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