Key adhesin gene in community-acquired methicillin-resistant Staphylococcus aureus

Otsuka, Taketo; Saito, Kohei; Dohmae, Soshi; Takano, Tomomi; Higuchi, Wataru; Takizawa, Yui; Okubo, Takeshi; Iwakura, Nobuhiro; Yamamoto, Tatsuo

doi:10.1016/j.bbrc.2006.06.038

Cited by 42 publications

(36 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These isolates were positive for icaD, cna, ebpS, eno, fnbA, fnbB, clfA and clfB, and were negative for icaA, bap, bbp and fib. The result for ebpS, eno, fnbA, clfA and clfB is in agreement with what Otsuka et al (2006) found for MRSA strains with various ST types and originating from various countries. Taneike et al (2006) described that all MRSA strains derived from nosocomial outbreaks in Japan were negative for fnbB, and nearly all of them harboured bbp.…”

Section: Resultssupporting

confidence: 79%

Screening of genes encoding adhesion factors and biofilm formation inStaphylococcus aureusisolates from poultry

Nemati¹,

Hermans²,

Devriese³

et al. 2009

Avian Pathology

View full text Add to dashboard Cite

Staphylococcus aureus isolates recovered from poultry between 1970 and 1972 (90 old isolates) and in 2006 (81 recent isolates) were screened for the presence of bap, icaA and icaD genes associated with biofilm formation, and for bbp, cna, ebpS, eno, fib, fnbA, fnbB, clfA and clfB genes that encode microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). Most of the old isolates were collected from broiler breeders affected by staphylococcal tenosynovitis and arthritis, whilst nearly all recent isolates were derived from the nose and cloaca of healthy broilers. Ten recent isolates belonged to the animalassociated methicillin-resistant S. aureus (MRSA) ST398. All isolates were positive for clfA, clfB, eno and fnbA, and were negative for bap and bbp. All recent isolates were also positive for ebpS and cna, and all old isolates were positive for fib. The average number of genes encoding adhesins and biofilm-associated proteins in both groups was eight per isolate. All MRSA ST398 isolates in this study were positive for icaD, cna, ebpS, eno, fnbA, fnbB, clfA and clfB. No relevant differences were found between the presence of adhesin and biofilm formation genes in old and recent S. aureus isolates or in isolates from healthy compared with diseased chickens. From this study, there is no indication that the presence of these genes has changed over time and no specific association could be found between the presence of certain MSCRAMM or biofilm genes in poultry S. aureus isolates and the isolate's capacity to cause disease.

show abstract

Section: Resultssupporting

confidence: 79%

Screening of genes encoding adhesion factors and biofilm formation inStaphylococcus aureusisolates from poultry

Nemati¹,

Hermans²,

Devriese³

et al. 2009

Avian Pathology

View full text Add to dashboard Cite

show abstract

“…Forty-one staphylococcal virulence genes (except the lukE-lukD genes) were detected by PCR by using the primers reported previously. The targeted genes were 3 leucocidin genes (29,40), 5 hemolysin genes (29), 17 staphylococcal enterotoxin (SE) genes (4,14,23,28,43,70), 1 putative staphylococcal enterotoxin gene (34), 3 exfoliative toxin genes (4,66), an exotoxin-like gene cluster (63), the epidermal cell differentiation inhibitor gene (29), and 14 adhesin genes (39,44,55,58,60). The lukE-lukD genes were assayed with primers LUKED-F1 (5Ј-CAGATGTGAAGGGTAGTGGA) and LUKED-R5 (5Ј-TCATTATCAGATGTTGCTGTTG), designed in this study.…”

Section: Methodsmentioning

confidence: 99%

Novel Characteristics of Community-Acquired Methicillin-Resistant Staphylococcus aureus Strains Belonging to Multilocus Sequence Type 59 in Taiwan

Takano

Higuchi

Otsuka

et al. 2008

Antimicrob Agents Chemother

Self Cite

151

View full text Add to dashboard Cite

show abstract

“…Forty-one staphylococcal virulence genes were detected by PCR using the previously reported primers. The targeted genes were three leucocidin genes (26,34), five hemolysin genes (26), 16 staphylococcal enterotoxin genes (3,23,25,39,57), one putative staphylococcal enterotoxin gene (28), three exfoliative toxin genes (3,55), an exotoxin-like gene cluster (54), the epidermal cell differentiation inhibitor gene (26), and 14 adhesin genes (32,40,50,51,53).…”

Section: Methodsmentioning

confidence: 99%

“…EB00449 was, however, slightly divergent in terms of plasmid profile from previously characterized PVL ϩ CA-MRSA ST30 strains, i.e., strain NN1, isolated from an infant (aged 11 months) with bullous impetigo (48), and strain NN12, carrying a conjugative drug resistance plasmid, isolated from a high school student (a basketball player aged 18 years) with bacteremia and osteomyelitis (40,48 a The number of strains tested was 22. They included the 13 strains with characteristics outlined in Table 1 and the 7 strains with characteristics outlined in Table 2.…”

Section: Pvlmentioning

confidence: 96%

Molecular Characteristics and In Vitro Susceptibility to Antimicrobial Agents, Including the Des-Fluoro(6) Quinolone DX-619, of Panton-Valentine Leucocidin-Positive Methicillin-Resistant Staphylococcus aureus Isolates from the Community and Hospitals

Yamamoto

Dohmae

Saito

et al. 2006

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

Highly virulent, community-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains with Panton-Valentine leucocidin (PVL) genes have been found increasingly worldwide. Among a total of 2,101 MRSA strains isolated from patients in hospitals in Japan, two were positive for PVL genes. One strain was identified as a community-acquired MRSA strain with genotype sequence type 30 (ST30) and spa (staphylococcal protein A gene) type 19 from Japan and was resistant only to ␤-lactam antimicrobial agents. The other strain was closely related to PVL ؉ multidrug-resistant, hospital-acquired MRSA strains (ST30, spa type 43) derived from nosocomial outbreaks in the 1980s to 1990s in Japan but with a divergent sequence type, ST765 (a single-locus variant of ST30). Twenty-two PVL ؉ MRSA strains, including those from Japan and those from other countries with various sequence types (ST1, ST8, ST30, ST59, and ST80) and genotypes, were examined for susceptibility to 31 antimicrobial agents. Among the agents, DX-619, a des-fluoro(6) quinolone, showed the greatest activity, followed by rifampin and sitafloxacin, a fluoroquinolone. The data suggest that DX-619 exhibits a superior activity against PVL ؉ MRSA strains with various virulence genetic traits from the community as well as from hospitals.

show abstract

Key adhesin gene in community-acquired methicillin-resistant Staphylococcus aureus

Cited by 42 publications

References 31 publications

Screening of genes encoding adhesion factors and biofilm formation inStaphylococcus aureusisolates from poultry

Screening of genes encoding adhesion factors and biofilm formation inStaphylococcus aureusisolates from poultry

Novel Characteristics of Community-Acquired Methicillin-Resistant Staphylococcus aureus Strains Belonging to Multilocus Sequence Type 59 in Taiwan

Molecular Characteristics and In Vitro Susceptibility to Antimicrobial Agents, Including the Des-Fluoro(6) Quinolone DX-619, of Panton-Valentine Leucocidin-Positive Methicillin-Resistant Staphylococcus aureus Isolates from the Community and Hospitals

Contact Info

Product

Resources

About