Key details of the duodenal-jejunal bypass in type 2 diabetes mellitus rats

Abstract

Using a number of fast-track and damage control surgical techniques, we have successfully established a stable model of gastric bypass in diabetic rats.

Establishing a successful rat model of duodenal- jejunal bypass: A detailed guide

“…The mortality rates have been rarely reported in previous DJB studies; available data show up to 35% mortality but rates vary depending on technical variants of the procedure. 17,37 Long-term post-operative survival has also not been reported in previous studies.…”

“…Technical variants in rodents include modifications of the limb length 16 or performing anastomosis between the distal stomach and jejunum (rather than between the duodenum and jejunum). 17 DJB has also been performed in combination with sleeve gastrectomy (vertically oriented resection of two-thirds of gastric volume). 18 Since its first description in 2004, 5 DJB has been used as an experimental model for investigations of mechanisms of gastrointestinal physiology as well as effects of gastrointestinal surgery on T2DM, 5,[19][20][21] hepatic metabolism, [22][23][24][25] kidney 26 and cardiovascular disease.…”

Comparison between Roux-en-Y and Duodenal-jejunal Bypass on hyperglycemia improvements in SD rats

“…First, the same incision was made. Then duodenum was transected 2 mm distal to pylorus, which, according to Han et al [8], was critical to operation success rate. Jejunum was also cut 8 cm from ligament of Treitz and the distal part was connected to pylorus with an end-to-end anastomosis using 7-0 silk suture.…”

“…In particular, food intake on the second day postoperative was noted to observe rats eating behavior after invasive damage. Food intake rate was calculated by the following equation: food intake rate=daily food consumption (g)/rat body weight (kg) [8]. Fasting plasma GLP-1 levels were assayed with an ELISA kit used strictly by the manufacturer"s instructions.…”

“…Quite a few studies suggest that the improvement can be attributed to the increase of GLP-1, which has multiple synergistic effects on glucose-stimulated insulin secretion [3,7] .Duodenal-jejunal bypass (DJB), a more recently applied procedure for T2DM, is also a potential alternative treatment for T2DM, especially those unresponsive to other modalities [8] . In the discussion of mechanism, GLP-1 also turn out to play a key role.…”