Key factors affecting the activity and stability of enzymes in ionic liquids and novel applications in biocatalysis

Gao, Wei‐Wei; Zhang, Fengxiu; Zhang, Guang‐Xian; Zhou, Cheng‐He

doi:10.1016/j.bej.2015.03.005

Cited by 116 publications

(78 citation statements)

References 144 publications

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“…However, ILs are not traditional high‐charge density salts well‐characterized by the Hofmeister series; therefore, studies to better understand the ILs effect on proteins at the molecular level are still ongoing. Up to date, cation/anion chemical structures, hydrophobicity, polarity, viscosity, hydrogen bond basicity/acidity, among others properties have been described to influence the enzymes activity and stability . Overall, these features contribute to specific interactions of the IL ions and the amino acids residues and solvation layer at the proteins surface .…”

Section: Introductionmentioning

confidence: 99%

“…Up to date, cation/anion chemical structures, hydrophobicity, polarity, viscosity, hydrogen bond basicity/acidity, among others properties have been described to influence the enzymes activity and stability. 42,43 Overall, these features contribute to specific interactions of the IL ions and the amino acids residues and solvation layer at the proteins surface. 44,45 However, the multitude of IL ion-water-protein interactions cannot be generalized, and more studies are required in this field to better understand the ILs impact on enzymes.…”

mentioning

confidence: 99%

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Effects of phosphonium‐based ionic liquids on the lipase activity evaluated by experimental results and molecular docking

Barbosa

Freire

Souza

et al. 2019

Biotechnology Progress

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In this work, the effect of several phosphonium‐based ionic liquids (ILs) on the activity of lipase from Burkholderia cepacia (BCL) was evaluated by experimental assays and molecular docking. ILs comprising different cations ([P4444]+, [P444(14)]+, [P666(14)]+) and anions (Cl−, Br−, [Deca]−, [Phosp]−, [NTf2]−) were investigated to appraise the individual roles of IL ions on the BCL activity. From the activity assays, it was found that an increase in the cation alkyl chain length leads to a decrease on the BCL enzymatic activity. ILs with the anions [Phosp]− and [NTf2]− increase the BCL activity, while the remaining [P666(14)]‐based ILs with the Cl−, Br−, and [Deca]− anions display a negative effect on the BCL activity. The highest activity of BCL was identified with the IL [P666(14)][NTf2] (increase in the enzymatic activity of BCL by 61% at 0.055 mol·L−1). According to the interactions determined by molecular docking, IL cations preferentially interact with the Leu17 residue (amino acid present in the BCL oxyanion hole). The anion [Deca]− has a higher binding affinity compared to Cl− and Br−, and mainly interacts by hydrogen‐bonding with Ser87, an amino acid residue which constitutes the catalytic triad of BCL. The anions [Phosp]− and [NTf2]− have high binding energies (−6.2 and −5.6 kcal·mol−1, respectively) with BCL, and preferentially interact with the side chain amino acids of the enzyme and not with residues of the active site. Furthermore, FTIR analysis of the protein secondary structure show that ILs that lead to a decrease on the α‐helix content result in a higher BCL activity, which may be derived from an easier access of the substrate to the BCL active site.

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Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Effects of phosphonium‐based ionic liquids on the lipase activity evaluated by experimental results and molecular docking

Barbosa

Freire

Souza

et al. 2019

Biotechnology Progress

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“…According to Figure 1 and Figure 2, the most frequently used terms are catalysis [27,29], homogeneous catalyst [30], heterogeneous catalysis [31,32,33], biosynthesis [34], esterification [27,31,35], transesterification [36,37,38], enzyme synthesis [39,40], microalga. Additionally, the most exploited synthetic routes are by transesterification and esterification.…”

Section: World Biodiesel Productionmentioning

confidence: 99%

Biocides Used as Additives to Biodiesels and Their Risks to the Environment and Public Health: A Review

et al. 2018

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One of the advantages of using biodiesel and its blends with diesel oil is the lower levels of emissions of particulate matter, sulfur dioxide, carbon monoxide, among others, making it less harmful to the environment and to humans. However, this biofuel is susceptible to microbial contamination and biodeterioration. In this sense, studies on the use of effective low toxicity biocides are being carried out, and this work aims to present the latest information (2008–2018) available in the scientific databases, on the use of biocides in biodiesel, mainly concerning their toxicity to the environment and public health. The results showed that in relation to the control of microbial contamination, the current scenario is limited, with seven publications, in which the most studied additives were isothiazolinones, oxazolidines, thiocyanates, morpholines, oxaborinanes, thiocarbamates and phenolic antioxidants. Studies regarding direct experiments with humans have not been found, showing the need for more studies in this area, since the potential growth of biodiesel production and consumption in the world is evident. Thus, there are need for more studies on antimicrobial products for use in biodiesel, with good broad-spectrum activity (bactericidal and fungicidal), and further toxicological tests to ensure no or little impact on the environment.

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“…mFADs) or requirement for additional protein partners and coenzymes. The only known exception among the FAmodifying enzymes is the lipases, which do not require a coenzyme, are usually soluble, and have been shown to be stable and active in a variety of different environments (including organic and ionic solvents) [174,175]. The use of lipases from microorganisms and animals has been widely established on an industrial scale [176]; for example, more than 10,000 tons of pure 1-phenylethylamine enantiomer are produced annually using lipase as a catalyst [177].…”

Section: Applications Of Fa-modifying Enzymesmentioning

confidence: 99%

Biotechnological potential of insect fatty acid-modifying enzymes

Tupec

Buček

Valterová

2017

Zeitschrift Für Naturforschung C

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There are more than one million described insect species. This species richness is reflected in the diversity of insect metabolic processes. In particular, biosynthesis of secondary metabolites, such as defensive compounds and chemical signals, encompasses an extraordinarily wide range of chemicals that are generally unparalleled among natural products from other organisms. Insect genomes, transcriptomes and proteomes thus offer a valuable resource for discovery of novel enzymes with potential for biotechnological applications. Here, we focus on fatty acid (FA) metabolism-related enzymes, notably the fatty acyl desaturases and fatty acyl reductases involved in the biosynthesis of FA-derived pheromones. Research on insect pheromone-biosynthetic enzymes, which exhibit diverse enzymatic properties, has the potential to broaden the understanding of enzyme specificity determinants and contribute to engineering of enzymes with desired properties for biotechnological production of FA derivatives. Additionally, the application of such pheromone-biosynthetic enzymes represents an environmentally friendly and economic alternative to the chemical synthesis of pheromones that are used in insect pest management strategies.

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Key factors affecting the activity and stability of enzymes in ionic liquids and novel applications in biocatalysis

Cited by 116 publications

References 144 publications

Effects of phosphonium‐based ionic liquids on the lipase activity evaluated by experimental results and molecular docking

Effects of phosphonium‐based ionic liquids on the lipase activity evaluated by experimental results and molecular docking

Biocides Used as Additives to Biodiesels and Their Risks to the Environment and Public Health: A Review

Biotechnological potential of insect fatty acid-modifying enzymes

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