Nurses are well-known for their dedication to hard work, performing multiple roles of nurse, manager, partner, parent and so on. To promote their work and life satisfaction, this study examined the interactive effects positive spillover (PSP), negative spillover (NSP), compensation (COM) and segmentation (SEG) caused on the interfaces between multiple roles and work and life satisfaction. In 2015, we collected a total of 910 valid data (male=118 female=792) using the academic online survey service. Questionnaire was composed of the work and life satisfaction items, the Multiple Roles Map (MRM) Questionnaire and demographic items. A logistic regression analysis (LRA) supported the significant relationship between predictor and outcomes and led to five conclusions; (1) The NSP effects were more strongly associated with higher work satisfaction than the PSP effects among male nurses, (2) The PSP effects were more strongly connected with work satisfaction than the NSP effects among female nurses. (3) The male nurses coped with negative events in family and private roles by positive events in nurse role. (4) The female nurses compensated the negative events in nurse roles by positive events in family and private roles. (5) Enrichment of the manager role among male nurses and that of the partner role among female nurses will be meaningful to promote their work and life satisfaction.