Keystone Taxa Lactiplantibacillus and Lacticaseibacillus Directly Improve the Ensiling Performance and Microflora Profile in Co-Ensiling Cabbage Byproduct and Rice Straw

Abstract

Ensiling has been widely applied to cope with agricultural solid waste to achieve organic waste valorization and relieve environmental pressure and feedstuff shortage. In this study, co-ensiling of cabbage leaf byproduct and rice straw was performed with inoculation of Lactiplantibacillusplantarum (LP) to investigate the effects of inoculation on ensiling performance and microflora profiles. Compared to the control, LP inoculation preserved more dry matter (DM) content (283.4 versus 270.9 g·kg−1 fresh matter (FM) on day 30), increased lactic acid (LA) content (52.1 versus 35.8 g·kg−1 dry matter on day 15), decreased pH (3.55 versus 3.79 on day 15), and caused accumulation of acetic acid (AA), butyric acid (BA), and ammonia. The investigation showed that LP inoculation modified microflora composition, especially resisting potential pathogens and enriching more lactic acid bacteria (LAB) (p < 0.05). Moreover, Lactiplantibacillus and Lacticaseibacillus were identified as the keystone taxa that influenced physicochemical properties and interactions in microflora. They were also the main functional species that directly restrained undesirable microorganisms (p < 0.05), rather than indirectly working via metabolite inhibition and substrate competition (p > 0.05). The results of this present study improve the understanding of the underlying effect of LP inoculation on improving silage quality and facilitate the bio-transformation of cabbage byproduct and rice straw as animal feed.

Community Synergy of Lactic Acid Bacteria and Cleaner Fermentation of Oat Silage Prepared with a Multispecies Microbial Inoculant

“…Clearly, specific LAB inoculants have their unique functions. For instance, as a homofermentative LAB, Pediococcus acidilactici occupies a dominant position during the initial period of fermentation, resulting in the production of lactic acid to inhibit spoilage organisms ( 14 ), and as a functional genus of LAB in silage, Lacticaseibacillus can also directly restrain the growth of undesirable microorganisms ( 15 ). In addition, Lactiplantibacillus , one of the most commonly used inoculants, can rapidly produce lactic acid and lower the pH in forage fermentation, and Lentilactobacillus can enhance the aerobic stability of various forages ( 16 ).…”

Dynamic Changes in Fermentation Quality and Structure and Function of the Microbiome during Mixed Silage of Sesbania cannabina and Sweet Sorghum Grown on Saline-Alkaline Land

“…However, the application of Sesbania cannabina in the silage industry is limited because of its low water-soluble carbohydrate (WSC) content (less than 5% DM) and high buffer capacity. On the other hand, coensiling is a common practice to improve fermentation quality and enhance the stability of the fermentation system ( 7 , 8 ). Numerous studies have reported the benefits of coensiling of sweet sorghum [ Sorghum dochna (Forssk.)…”

ISOLATION OF Bacillus subtilis CAPABLE OF DEGRADING AGRICULTURAL WASTES

“…It is essential for plant growth and food and feeds production since N2 is required for the cell production of enzymes, proteins, photosynthesis, Chromosomes, and RNA. To supply legumes with nitrogen, rhizobia bacteria use nitrogenase, an enzyme that converts N2 from the atmosphere into nitrate [28,29]. The physiological nitrification in (BNF) process is responsible for 65 percent of the nitrogen now used in agriculture and will continue to play important role in future sustainable crop production systems.…”