Ki-67 immunolabeling in pre-malignant lesions and carcinoma of the prostate. Histological correlation and prognostic evaluation

Muñoz, Elisa; Gómez, Francisco Javier Torres; Paz, J.I.; Casado, I.

doi:10.4081/816

Cited by 23 publications

(21 citation statements)

References 22 publications

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“…The MIB-1 staining index has been associated with PCa patient survival (27,28,33,41). In our present study, we found no correlation of MIB-1 index in prostate needle biopsies with pathologic findings in RP tissue sections.…”

Section: Resultscontrasting

confidence: 73%

“…Aggressive PCa was identified by the increased ratio of CB: SA together with pelvic lymph node metastasis (23). The MIB-1 staining index has been associated with PCa patient survival (27,28,33,41). DNA ploidy adds useful prognostic information for some PCa patients (19,32,(42)(43)(44).…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, the technique involved the initial photographing of cancerous and BPH glands followed by covering sections with transparent 100 m-thick ethylene-vinyl acetate films, isolation of the selected areas, and their photography. Isolated glands and cells were homogenized for measuring CB and SA by an Elisa assay which is a quantitative sandwich enzyme immunoassay technique, also described by others [28][29][30] . Samples were added to a micro-titer plate that was pre-coated with a monoclonal antibody specific for CB and incubated overnight at 4 o C. The wells were washed with blocking solution, 5% BSA (bovine serum albumin) in PBS (phosphate buffer saline).…”

Section: Immunohistochemical Localization Of Cathepsin B and Stefin Amentioning

confidence: 99%

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Prediction of Aggressive Human Prostate Cancer by Cathepsin B

Sinha¹

2008

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. REPORT DATE 01-03-2008 REPORT TYPE 5e. TASK NUMBEREmail: sinha001@tc.umn.edu 5f. WORK UNIT NUMBER PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBERUniversity of Minnesota Minneapolis, MN 55455-2070 SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR'S ACRONYM(S) U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland 21702-5012 SPONSOR/MONITOR'S REPORT NUMBER(S) DISTRIBUTION / AVAILABILITY STATEMENTApproved for Public Release; Distribution Unlimited SUPPLEMENTARY NOTES14. ABSTRACT I received approval of the DOD-HSRRB in April, 2005 and began the study shortly after receiving approval. We are enclosing copies of published and accepted papers in the Anticancer Research. These papers address parts of both Specific Aims outlined in the project. I submitted an abstract of our accepted paper, "Cathepsin B Expression Indicates that Prostate Cancer is Similar in African-American and Caucasian Men" to the Department of Defense Prostate cancer research program meeting: Innovative Minds in Prostate Cancer Today (IMPACT). Our abstract was selected and accepted for presentation on September 5-6 meetings in Atlanta, GA. This is significant achievement for our work that started in April 2005. Our paper, "Heterogeneity of Cathepsin B and Stefin A Expression in Gleason Pattern 3+3 (score 6) Prostate Cancer Needle Biopsies" was published in Anticancer Research 27: 1407-1414, 2007. We showed that small foci of Gleason pattern 3+3 (histological score 6) tumors in needle biopsies have heterogeneous cathepsin B and stefin A immunostaining. We have shown that score 6 tumors are heterogeneous and some of the tumors had micrometastases. We have suggested that stratification of these tumors by cathepsin B and stefin A in relation to clinical data may assist in identification of aggressive cancer and treatment selection. SUBJECT TERMS Introduction:Cathepsin B (CB) is required for degradation of basement membrane and extracellular matrix proteins, including prostate cancer (PC) cell invasion to the subjacent stroma and extracellular matrix proteins followed by met...

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Section: Resultscontrasting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Immunohistochemical Localization Of Cathepsin B and Stefin Amentioning

confidence: 99%

See 1 more Smart Citation

Prediction of Aggressive Human Prostate Cancer by Cathepsin B

Sinha¹

2008

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“…Resistance to hormone therapy is related to a considerable proportion of PCa deaths with few therapeutic options available thereafter. 10,11 Recently, molecular tests for cancer cell-associated genes and other genetic markers, being direct indicators of the presence of the cancer cells in the biological specimen, have been predicted to provide improvement in diagnostic specificity 12,13 As the growth rate of tumour tissue is determined by proliferative activity and cell death, expression of two proliferative makers--Ki-67 and PCNA were examined in this study to enhance the facility and accuracy of early diagnosis of PCa.…”

Section: Discussionmentioning

confidence: 99%

Ki-67 and PCNA expression in prostate cancer and benign prostatic hyperplasia

Zhong¹,

Peng²,

He³

et al. 2008

CIM

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Clin Invest Med 2008; 31 (1): E8-E15. AbstractObjective: Ki-67 is a proliferation-associated nuclear antigen and is expressed in all cycling cells except for resting cells in the G0-phase. PCNA is an acidic nuclear protein and has been recognized as a histologic marker for the G1/ S phase in the cell cycle. Ki-67and PCNA labeling indices are considered to reflect cell proliferation, particularly, growth fraction. The purpose of this study is to investigate the expression levels of Ki-67 and PCNA in prostate cancer (PCa) and benign prostatic hyperplasia (BPH) and their potential on the early diagnosis of PCa. Methods: Human prostate cancer cell lines LNCaP and PC-3, human normal prostate epithelial cell line HuPEC, tissues from patients with PCa (121 cases) and BPH (45) and 36 normal cases were examined for the expression of Ki-67 and PCNA by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Then, the association of Ki-67 and PCNA expression with clinical grading of PCa was analyzed by immunohistochemistry staining. Results: The ratios of PCNA and Ki-67 expression levels in LNCaP and PC-3 were higher (P<0.05, P<0.001) than that in HuPEC. The two markers were differentially expressed in three tissues and showed increased expression in PCa (P<0.05) and BPH (P<0.05), relative to human normal prostate tissues. Compared with BPH, the ratio of Ki-67 and PCNA expressed in tumour tissue was increased (P<0.05). The increase of Ki-67 was greater than that of PCNA. Expression of the two markers increased after different grading of PCa cases Prostate cancer (PCa) is the second most frequent cause of male cancer-related death in the United States of America (USA) and Western Europe. Its incidence is continuously rising, with over 200 000 new cancers and 35 000~40 000 deaths per year, 1 Carcinogenesis and the mechanisms influencing the progression and prognosis of PCa is a multistep process, involving both genetic insults to epithelial cells and changes in epithelial-stromal interactions. 2 Despite extensive research PCa is not understood.There are two proliferative markers-Ki-67 and proliferating cell nuclear antigen (PCNA). Ki-67 antigen is expressed in proliferative cells throughout the G1, S, G2, and M phases, and provides a reliable index of cellular proliferation 3,4 PCNA is an acidic nuclear protein, expressed mainly in phase S of the cellular cycle. It becomes active, in various tissues par-

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“…Ki-67 is a widely accepted proliferation marker; it is strictly associated with cell proliferation due to it being expressed during all active phases of the cell cycle (G 1 , S, G 2 and mitosis), but absent in resting cells (G 0 ) (Scholzen & Gerdes 2000;Muñoz et al 2003).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Sex-based differences in lymphocyte proliferation in the spleen after vanadium inhalation

Rodríguez-Lara

Cambas

Villalva

et al. 2016

Journal of Immunotoxicology

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Vanadium (V) is a transition metal often adhered to particulate matter and released into the atmosphere as vanadium pentoxide (V 2 O 5 ) by the burning of fossil fuels. This air pollutant causes adverse effects in the immune system. Lymphocytosis and splenomegaly have been reported with increased white pulp in mice after V inhalation. The effect of V on the immune system as related to sex has been poorly investigated. This study sought to determine if V inhalation (a) produced lymphoproliferation that could explain the changes previously observed in the spleen and in peripheral blood lymphocyte counts and (b) whether any observed effects differed due to gender. Immunohistochemical analyses of Ki-67, a specific proliferation marker, was made in the spleens of CD-1 male and female mice exposed for 1 h, twice a week, over a 12-week period to V 2 O 5 (at 1.4 mg V 2 O 5 /m 3 ) by whole-body inhalation; similar analyses were performed on spleens of control mice exposed to vehicle (filtered air). The results showed that in male mice there was a significant increase in percentage of Ki-67 immunopositive lymphocytes starting from the second week and until the end of the exposure. The Ki-67 signal was cytoplasmic and nuclear in the exposed males, while in controls the signal was only nuclear. In female mice, V inhalation singificantly increased the percentage of proliferating lymphocytes only after 1 week of exposure. Ki-67 signal was observed only in the nucleus of lymphocytes from the control and exposed females. The results here help to explain the splenomegaly and lymphocytosis observed previously in male mice and support the lymphoproliferative effect induced by V. Lastly, the finding that there was a sex difference in the effect of vanadium on lymphocyte proliferation suggests a role for sex hormones in potential protection against V immunotoxicity; however, further studies are needed to support this hypothesis. ARTICLE HISTORY

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Ki-67 immunolabeling in pre-malignant lesions and carcinoma of the prostate. Histological correlation and prognostic evaluation

Cited by 23 publications

References 22 publications

Prediction of Aggressive Human Prostate Cancer by Cathepsin B

Prediction of Aggressive Human Prostate Cancer by Cathepsin B

Ki-67 and PCNA expression in prostate cancer and benign prostatic hyperplasia

Sex-based differences in lymphocyte proliferation in the spleen after vanadium inhalation

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