Kidney protection by pretreatment with free radical scavengers and allopurinol: Renal function at recirculation after warm ischaemia in rabbits

Hansson, R.; Johansson, Sven Erik; Jonsson, Olof; Pettersson, S.; Scherstén, T; Waldenström, J

doi:10.1042/cs0710245

Cited by 33 publications

(19 citation statements)

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“…[40] showed that SOD was able to inhibit the postischemic increase in vascular permeabili ty. Hansson et al [41] showed a protective effect in the kidney after ischemia by radical scavengers, such as SOD and mannitol. In the hamster cheek pouch.…”

Section: Discussionmentioning

confidence: 99%

Effects of Oral Administration of Purified Micronized Flavonoid Fraction on Increased Microvascular Permeability Induced by Various Agents and on Ischemia/Reperfusion in Diabetic Hamsters

Donyo¹

1995

Int J Microcirc

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The effects of a clinically used purified micronized flavonoid fraction (S5682) containing 90% diosmin and 10% hesperidin on increased microvascular permeability induced by histamine, bradykinin and leukotriene B4 (LTB4) were investigated by intravital microscopy in the cheek pouch preparation of diabetic hamsters. We also investigated the effects of S 5682 on macromolecular permeability increase and leukocyte adhesion during ischemia-reperfusion using the same preparation. Diabetes was induced by an intraperitoneal injection of streptozotocin (50 mg/kg). S 5682, suspended in 10% lactose solution, or vehicle (10% lactose) was administered orally for 25 days at 20 mg/kg/day (10 mg/kg twice a day), starting 5 days after the streptozotocin injection. Fluorescein isothiocyanate-labelled dextran (molecular weight 150,000) was given intravenously, 30 min after completion of the cheek pouch preparation. The leukocytes were stained by continuous intravenous infusion of acridine orange (0.5 mg/kg/min). Histamine (2 µM), bradykinin (1 µM), and LTB₄ (0.01 µM), applied topically for 5 min, increased the number of fluorescent vascular leakage sites in postcapillary venules. A temporary ischemia (duration: 30 min) with total circulatory arrest of the cheek pouch was obtained by clamping the neck of the everted pouch. The maximum number of leaky sites (per cm² in the prepared area) which occurs either at 5 min after the beginning of each topical application or 10 min after the onset of reperfusion was quantified in UV light microscopy. The results from 60 animals divided into ten groups of 6 animals each are presented as means ± SEM. In comparison with vehicle, S 5682 significantly inhibited the macromolecular permeability increasing the effect of histamine (343.8 ± 18.5 vs. 91.0 ± 8.2 leaks/cm²; p < 0.001), bradykinin (347.0 ± 14.6 vs. 110.3 ± 8.5 leaks/cm²; p < 0.001) and LTB₄ (323.0 ± 15.5 vs. 161.3 ± 13.8 leaks/cm²; p < 0.001). At reperfusion, after 30 min ischemia, S 5682 significantly decreased the observed macromolecular permeability (168.5 ± 19.7 vs. 52.7 ± 6.3 leaks/cm²; p < 0.01). Flavonoid-treated animals also tended to have a lower number of leukocytes adhering to the venular endothelium (104.8 ± 11.0 vs. 75.8 ± 9.7/6 mm²; p > 0.05). These results demonstrate that oral administration of S 5682 for 25 days at 20 mg/kg body weight/day has a protective effect on leakage of macromolecules after application of permeability-increasing substances and during ischemia-reperfusion in the cheek pouch microvasculature of diabetic hamsters. In conclusion, the present data illustrating the inhibitory effect of a clinically relevant dose of S 5682 on the inflammatory processes induced in this in vivo model of microcirculation may serve as a rational basis to explain its clinical efficacy.

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Section: Discussionmentioning

confidence: 99%

Effects of Oral Administration of Purified Micronized Flavonoid Fraction on Increased Microvascular Permeability Induced by Various Agents and on Ischemia/Reperfusion in Diabetic Hamsters

Donyo¹

1995

Int J Microcirc

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“…Several drugs have been studied in the protection of the kidney from I/R injury (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13). The anti-anginal medication trimetazidine (TMZ) has been shown to protect the myocardium cells through inhibition of fatty acid oxidation and reciprocal activation of pyruvate oxidation, resulting in less production and accumulation of lactate and hydrogen cation, H+, during ischaemia (14).…”

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confidence: 99%

Comparative study between trimetazidine and ice slush hypothermia in protection against renal ischemia/reperfusion injury in a porcine model

et al. 2011

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Purpose: The aim of the study was to compare the effects of renal ice slush hypothermia and the use of trimetazidine in the protection against ischemia/reperfusion (I/R) injury. Materials and Methods: Fifteen farm pigs were submitted to left kidney ischemia and right nephrectomy during the same procedure. Animals were divided into three groups. Group 1 was submitted to warm ischemia; Group 2 was submitted to cold ischemia with ice slush; and Group 3 received trimetazidine 20 mg one day and 4 hours before surgery. Ischemia time was 120 minutes in all three groups. Serum creatinine (SCr) and plasma iohexol clearance (CLioh) were measured before surgery and on postoperative days (PODs) 1,3,7, and 14. Semi-quantitative analyses of histological alterations were performed by a pathologist. A p value of < 0.05 was considered significant. Results: All groups showed elevation of serum creatinine in the first week. Serum creatinine was higher in Group 3 in the first and third postoperative days (Mean Cr: 5.5 and 8.1 respectively). Group 2 showed a lower increase in creatinine and a lower decrease in iohexol clearance than the others. Renal function stabilized in the fourteenth POD in all three groups. Analyses of histological alterations did not reach statistical significance between groups. Conclusion: Trimetazidine did not show protection against renal I/R injury in comparison to warm ischemia or hypothermia in a porcine model submitted to 120 minutes of renal ischemia.

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“…Several reports show that antioxidants protect while others show no protection [51][52][53][54]. To clarify this problem and better understand how anoxia affects the sensitivity to oxidant injury, we have performed in vitro studies in which isolated hepatocytes are exposed to both controlled periods of anoxia and controlled oxidant loads.…”

Section: Ischemia Reoxygenation Injury and The Selective Vulnerabilimentioning

confidence: 99%

Intracellular Oxygen Supply: Implications for Intensive Care

Jones¹,

Aw²,

Kowalski³

1991

Update in Intensive Care and Emergency Medicine

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Kidney protection by pretreatment with free radical scavengers and allopurinol: Renal function at recirculation after warm ischaemia in rabbits

Cited by 33 publications

References 0 publications

Effects of Oral Administration of Purified Micronized Flavonoid Fraction on Increased Microvascular Permeability Induced by Various Agents and on Ischemia/Reperfusion in Diabetic Hamsters

Effects of Oral Administration of Purified Micronized Flavonoid Fraction on Increased Microvascular Permeability Induced by Various Agents and on Ischemia/Reperfusion in Diabetic Hamsters

Comparative study between trimetazidine and ice slush hypothermia in protection against renal ischemia/reperfusion injury in a porcine model

Intracellular Oxygen Supply: Implications for Intensive Care

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