Kidney‐Replenishing Herb Induces SOCS‐3 Expression via ERK/MAPK Pathway and Improves Growth of the First‐Trimester Human Trophoblast Cells

Xing, Cheng; Zhang, Dexiang; Shen, Guanghui; Tao, Minfang

doi:10.1155/2017/2473431

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…The ERK/MAPK pathway is an important signaling cascades that is involved in cell proliferation and embryo development (45). We found that collagen I downregulated ERK phosphorylation in HTR-8/SVneo cells and mouse placenta compared with the control.…”

Section: Discussionmentioning

confidence: 72%

Collagen I Induces Preeclampsia-Like Symptoms by Suppressing Proliferation and Invasion of Trophoblasts

et al. 2021

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Preeclampsia is a common obstetric disorder affecting 2-8% of pregnancy worldwide. Fibrosis is an important histological change occurring in preeclamptic placenta, and might depend on the excess deposition of collagen I. However, the role of fibrotic placenta and collagen I in the pathogenesis of preeclampsia remains unclear. Therefore, we analyzed the collagen deposition and the expression of Collagen I in human placenta by Masson staining, Sirius red staining and western blotting. Further, the role of collagen I in preeclampsia pathogenesis was studied in C57BL/6 mice. HTR-8/SVneo cells were used to investigate the mechanisms underlying the effects of collagen I in trophoblasts by transcriptome sequencing and pharmacological agonists. Human preeclamptic placenta exhibited a significantly higher degree of fibrosis in stem villi and terminal villi than normal placenta, and was characterized by collagen I deposition. In vivo, a single injection of collagen I on gestational day 0.5 led to an increase in systolic pressure of pregnant mice from gestational days 4.5–17.5, to a decrease in weight and number of embryos, and to enhanced placental collagen I expression and degree of fibrosis compared with control mice. In vitro, collagen I attenuated the proliferation and invasion of HTR-8SV/neo cells. This effect could be reversed by treatment with agonists of ERK and β-catenin. Moreover, transcriptome sequencing demonstrated that signaling pathways related to cell proliferation and invasion were significantly downregulated in HTR-8SV/neo cells. Thus, we propose that collagen I induced preeclampsia-like symptoms by suppressing the proliferation and invasion of trophoblasts through inhibition of the ERK phosphorylation and WNT/β-catenin signaling pathways. Our findings could pave the way to the discovery of small-molecule inhibitors for preeclampsia treatment and future studies with larger sample size are required.

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Section: Discussionmentioning

confidence: 72%

Collagen I Induces Preeclampsia-Like Symptoms by Suppressing Proliferation and Invasion of Trophoblasts

et al. 2021

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“…Suppressors of cytokine signaling (SOCS) proteins has been shown to play vital roles in modulating cell growth and migration processes [17]. Many SOCS proteins are often expressed in tissues or organs, contributing to the maintenance of normal tissue functions and homeostasis, which thus affect cell growth and migration [18]. Moreover, many tripartite motif containing proteins are implicated in cancerassociated cell growth and migration, and disorders of tripartite motif containing proteins play a vital role in the occurrence and progression of cancer [15].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of SOCS4 inhibits proliferation and migration of cervical cancer cells by regulating JAK1/STAT3 signaling pathway

Chen¹,

Chen²,

Yu³

et al. 2021

EJGO

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To delve into the related molecular mechanism of suppressor of cytokine signaling 4 (SOCS4) on cervical cancer cell proliferation and migration. Methods: Quantitative real-time polymerase chain reaction and western blot assays were employed to examine SOCS4 mRNA or protein expression in four human cervical cancer cell line (HeLa, SW-732, AV3, and CaSki) and normal cervical epithelium immortalized cell line (H8) MTT cell viability assays were applied to verify the cell proliferation of HeLa after overexpression of SOCS4. Wound scratch healing assays and transwell assays were applied to examine cell migration and invasion of HeLa after overexpression of SOCS4. Flow cytometry and western blot assays were applied to check the role of SOCS4 in the apoptosis of cervical cancer cells. The western blot assays were applied to examine the protein expression of JAK1, p-JAK1, STAT3, and p-STAT3 in HeLa after overexpression of SOCS4. Results: In this study, the results revealed that the mRNA and protein expression level of SOCS4 was lower in four human cervical cancer cell line than normal cervical epithelium immortalized cell line, respectively. Overexpression of SOCS4 inhibited the proliferation migration, and invasion of cervical cancer cells as well as promotes apoptosis of cervical cancer cells. Meanwhile, overexpression of SOCS4 in HeLa would inhibit phosphorylation of JAK1 and STAT3 protein. Conclusion: SOCS4 inhibited the proliferation and migration of cervical cancer cells by regulating the JAK1/STAT3 pathway.

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Kidney‐Replenishing Herb Induces SOCS‐3 Expression via ERK/MAPK Pathway and Improves Growth of the First‐Trimester Human Trophoblast Cells

Cited by 2 publications

References 36 publications

Collagen I Induces Preeclampsia-Like Symptoms by Suppressing Proliferation and Invasion of Trophoblasts

Collagen I Induces Preeclampsia-Like Symptoms by Suppressing Proliferation and Invasion of Trophoblasts

Overexpression of SOCS4 inhibits proliferation and migration of cervical cancer cells by regulating JAK1/STAT3 signaling pathway

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