KIF1B 2, Capable of Interacting with CHP, Is Localized to Synaptic Vesicles

Abstract

Kinesin family proteins are microtubule-dependent molecular motors involved in the intracellular motile process. Using a Ca2+ -binding protein, CHP (calcineurin B homologous protein), as a bait for yeast two hybrid screening, we identified a novel kinesin-related protein, KIF1Bbeta2. KIF1Bbeta2 is a member of the KIF1 subfamily of kinesin-related proteins, and consists of an amino terminal KIF1B-type motor domain followed by a tail region highly similar to that of KIF1A. CHP binds to regions adjacent to the motor domains of KIF1Bbeta2 and KIF1B, but not to those of the other KIF1 family members, KIF1A and KIF1C. Immunostaining of neuronal cells showed that a significant portion of KIF1Bbeta2 is co-localized with synaptophysin, a marker protein for synaptic vesicles, but not with a mitochondria-staining dye. Subcellular fractionation analysis indicated the co-localization of KIF1Bbeta2 with synaptophysin. These results suggest that KIF1Bbeta2, a novel CHP-interacting molecular motor, mediates the transport of synaptic vesicles in neuronal cells.

Transcriptome analysis of nicotine-exposed cells from the brainstem of neonate spontaneously hypertensive and Wistar Kyoto rats

“…50 Interestingly, Szolnoki et al, 51 showed that cognitive deficit in hypertensive smokers is related to the presence of polymorphisms in the kinesin gene, which may be related to the findings of this study in which the expression of kinesin 1Bbeta is 70% lower in SHR cells as compared with WKY and nicotine increased it in SHR and decreased it in WKY cells.…”

Solution Structure of the Cytoplasmic Region of Na+/H+ Exchanger 1 Complexed with Essential Cofactor Calcineurin B Homologous Protein 1

“…It was initially identified as a protein (p22) involved in vesicular transport (15) and the inhibition of calcineurin phosphatase activity (16). It was also found to interact with microtubules (17), DRAK2 (death-associated protein kinaserelated apoptosis-inducing protein kinase 2) (18) and KIF1B␤2 (kinesin family 1B␤2) (19).…”

“…3B). The secondary structure consists of ␣A (residues [11][12][13][14][15][16][17][18][19][20][21][22], ␣B (residues 26 -37), ␣C (residues 48 -51), ␣D (residues 64 -70), ␣E (residues 80 -88), ␣F (residues 111-122), ␣G (residues 132-143), ␣H (residues 149 -162), ␣I (residues 174 -180), and ␣J (residues 185-188) (Figs. 1B and 3B).…”

Calcineurin Homologous Protein Isoform 2 (CHP2), Na+/H+ Exchangers-Binding Protein, Is Expressed in Intestinal Epithelium.

“…Both rCHPs were found in the cytoplasm and also accumulated predominantly in a juxtanuclear region in which the Golgi and microtubule-organizing center are present. Since CHP1 has been shown to associate with microtubules and KIF1Bb2, one of kinesin moter proteins, 39,40) the accumulation of rCHPs in the juxtanuclear region may result from the microtubule-association. Although the localization of the two rCHPs was similar in the cytoplasm and juxtanuclear region, the fluorescence intensities of the two rCHPs were different in the region stained with Hoechst33342, a nuclear-staining dye (Fig.…”