KIF20A, highly expressed in immature hematopoietic cells, supports the growth of HL60 cell line

Abstract

A microtubule-associated motor protein, kinesin-like family member 20A (KIF20A; also called MKlp2) is required for cytokinesis and contributes to intracellular vesicular trafficking. KIF20A plays a critical role in the development of several cancers, but its role in blood cells and hematological malignancies have not been studied. In the present study, we focused on the role of KIF20A in hematopoietic cells and possible involvement in myeloid neoplasms. We found that human leukemia cell lines and normal bone marrow CD34-positive cells stimulated by growth factors, but not mature peripheral blood cells, exhibit high KIF20A expression. We further found that HL60 cells, which originally express a large amount of KIF20A, showed decreased KIF20A expression in parallel with both neutrophil-like and macrophage-like differentiation-induction. KIF20A-knockdown using a lentivirus shRNA transfection system led to partial cell cycle arrest at the G2/M phase and frequent appearance of multinucleated cells. Treatment with a KIF20A-selective inhibitor, paprotrain enhanced the multinuclearity of KIF20A-knockdown cell clones and suppressed growth. The present study contributes to our understanding of the role of KIF20A in blood cells and leukemia cells in particular.

Retracted: KIF20A silence inhibits the migration, invasion and proliferation of non‐small cell lung cancer and regulates the JNK pathway

“…12,13 Cancer cells overexpressed KIF20A can be recognized by hosts to initiate immune response, thereby KIF20-derived peptides could be used as a novel immunotherapy agent. 5,14 Several clinical trials using KIF20A vaccine had been performed. 15,16 However, no data are available to date about the role of KIF20A in NSCLC.…”

Identification of potential hub genes associated with the pathogenesis and prognosis of hepatocellular carcinoma via integrated bioinformatics analysis

“… 99 Kinesin family member 20A (KIF20A) is required during mitosis for the final step of cytokinesis. 100 , 101 Studies have found that high expression of KIF20A is correlated with progression or poor prognosis of many types of cancers. 102 – 104 Furthermore, KIF20A is aberrantly expressed in HCC tissues and its expression may be associated with poor OS.…”

Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression

“…KIF20A, also known as mitotic kinesin-like protein 2 (MKlp2) or rabkinesin6 (RAB6KIFL), is a kinesin-6 family 10 microtubule plus-end directed motor that is also necessary in the exit of mitosis for the final step of cytokinesis ( 25 ). KIF20A is found in the central spindle during mitosis, whose phosphorylation is necessary for cytoplasmic division ( 26 ).…”

“…The overexpression of KIF20A is linked to the onset, progression, and prognosis of different cancers. Recent researches have reported that KIF20A was upregulated in esophageal squamous cell carcinoma ( 28 ), lung adenocarcinoma ( 29 ), prostate cancer ( 30 ), cervical cancer ( 31 ), colorectal cancer ( 32 ), non-small cell lung cancer ( 33 ), gastric cancer ( 34 ), bladder cancer ( 35 ), renal clear cell carcinoma ( 36 ), breast cancer ( 37 ), hepatocellular carcinoma ( 38 ), nasopharyngeal carcinoma ( 39 ), ovarian cancer ( 40 ), leukemia ( 25 ), glioma ( 41 ), and soft tissue sarcoma ( 42 ). This is consistent with our analysis in the GEPIA online database.…”