Kıl keçisi abomasumunda ghrelin, obestatin ve leptin hormonlarının  immunohistokimyasal dağılımları

Karakoç, Zelal; Topaloğlu, Uğur; Ketani, M. Aydın

doi:10.15312/eurasianjvetsci.2019.248

Cited by 3 publications

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“…Obestatin immunoreactivity has been reported in the stomach in studies conducted in humans and various mammalian species (Dun et al, 2006;Grönberg et al, 2008;Tsolakis et al, 2009). In their study on hair goats, Karakoç et al (2019) reported that obestatin was detected in the cytoplasms of glandular cells in all three regions of the abomasum.…”

Section: Discussionmentioning

confidence: 96%

“…It has been reported that leptin is expressed in varying intensities in human gastric glands, ranging from moderate to strong, and immunoreactivity has been reported in the cells of the lower regions of the gastric glands and also in pepsinogen granules in the main cells (Cinti et al, 2000;Gülle et al, 2009). Karakoç et al (2019) reported that they found leptin immunoreactivity in all three regions of the abomasumin hair goatin a study they conducted. It was shown in a study on determining the distribution of leptin and leptin receptors in the tissues by PCR method in cattle, that leptin was not present in the abomasum at all (Chelikani et al, 2003).…”

Section: Discussionmentioning

confidence: 98%

“…Contrary to the studies that report that ghrelin is commonly found in humans, rodents, and pigs, Zhi-Guo et al ( 2006) has reported that ghrelin is rarely found in ruminants. In studies on ghrelin in sheep, Saanen dairy goats, and hair goats, it has been reported that ghrelin is synthesized from all regions of the abomasum (Karakoç et al, 2019;Özfiliz et al, 2011;Yu et al, 2012). In their study to determine ghrelin expression in the gastrointestinal tracts of early weaning lambs by PCR method, Wang et al (2014) reported that ghrelin expression was detected in the abomasum.…”

Section: Discussionmentioning

confidence: 99%

“…The hormones leptin, ghrelin and obestatin secreted from the stomach constitute one of these mechanisms with their effects on food intake. Leptin, ghrelin, and obestatin are defined as appetite‐regulating hormones (Karakoç et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Ghrelin is a 28 amino acid lipopeptide hormone secreted mainly from the fundus and pylorica regions of the stomach (Karakoç et al, 2019). It has been reported that this hormone is also expressed in organs such as the duodenum, jejunum, ileum, colon, brain, salivary glands, thyroid gland, heart, pancreas, kidneys, testicles, lungs, placenta, gonads, immune system, mammary glands and even teeth (İlhan & Erdost, 2009, Sağsöz et al, 2020Özfiliz et al, 2011).…”

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confidence: 99%

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Immunohistochemical distributions of leptin, ghrelin and obestatin hormones in bull and ram abomasum

Karakoç

Topaloğlu

Bayram

2022

Anat Histol Embryol

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In most mammals, leptin plays central and peripheral roles in a wide range of metabolic activities including feed consumption and digestion, energy expenditure and the regulation of carbohydrate‐fat accumulation. Ghrelin is a strong appetite‐stimulating hormone, while obestatin has the opposite effect of ghrelin. The latter is released during feeding, causing a sense of satiety. This study aimed to reveal if the metabolic hormones, as well as OB‐R and GHS‐R, were present in bull and ram abomasums. Streptavidin‐biotin peroxidase method was implemented to determine the expressions and localizations of metabolic hormones and their receptors, and obestatin. Immunoreactivity for leptin and obestatin was present in the cytoplasms of luminal and glandular cells, while smooth muscle cells were immunoreactant for ghrelin, in both bull and ram abomasums. Immunoreactivity for OB‐R was present in luminal and glandular cell, parietal cell cytoplasms, and blood vessels. Immunoreactivity for GHS‐R, on the other hand, was found in glandular epithelial cell cytoplasms and parietal cell cytoplasms, in both bull and ram abomasums. Leptin immunoreactivity was detected in the glandular epithelial cells and stroma, and particularly in the parietal cells. Ghrelin immunoreactivity was not observed in glandular epithelial cells. Obestatin immunoreactivity was found intensely in the bull abomasum, while moderate immunoreactivity was observed in the ram abomasums. As such, leptin, ghrelin and obestatin hormones were expressed in bull and ram abomasums, similar to the abomasums of other species. It is believed that these hormones play important roles in the digestive system activities and the regulation of energy in bulls and rams.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Immunohistochemical distributions of leptin, ghrelin and obestatin hormones in bull and ram abomasum

Karakoç

Topaloğlu

Bayram

2022

Anat Histol Embryol

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Histopathological examination of the placenta after delivery in pregnant women with COVID-19

Taş

ERDEMCİ

Aşır

et al. 2022

Journal of Health Sciences and Medicine

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Introduction: COVID-19 is a viral disease generated by a new coronavirus named SARS-CoV-2. The consequences of this virus on the human placenta and the newborn are still unclear. IL-6 can disturb the placenta's immunological homeostasis and be employed as an inflammatory marker for the poor prognosis of COVID-19 infection. Bax has some features like being a key protein regulating apoptotic mechanisms and plays an important role in both maintaining dynamic balance and integrity in the placenta as in many tissues. This study aims to indicate the impact of COVID-19 on inflammation and apoptotic pathways in the placenta by using IL-6 and Bax antibodies. Material and Method: COVID-19 positive (n:10) and COVID-19 negative (n:10) normotensive placentas were included. Haematoxylin-eosin staining and immunohistochemical staining (IL-6 and Bax antibodies) were applied. Statistical data of immunohistochemical (IL-6 and Bax expression) staining results were assessed by analyzing the H-score. Biochemical parameters were recorded. Group means were analyzed with a nonparametric Kruskal Wallis Test. Results: In the COVID-19 group, increased syncytial knots, fibrin deposition, inflammation, fibrinoid necrosis, neutrophil accumulation were observed. The COVID-19 group had considerably higher levels of IL-6 and Bax expression than the control group. Furthermore, COVID-19 patients had statistically lower WBC and higher CRP values than normotensive patients. Conclusion: COVID-19 has been linked to placental inflammation and trophoblast cell damage, both of which can result in major maternal and fetal problems during pregnancy. We found intense IL-6 expression in the placentas of pregnant women with COVID-19 infection. A rise in IL-6 levels triggers CRP production, and this increase is linked to the severity of COVID-19 as a risk factor. Also, we suggested that COVID-19 infection triggers the apoptotic process in placental tissue by increasing the expression of the proapoptotic Bax protein. It is clinically very significant to follow up COVID-19 positive pregnancies for maternal and fetal health. During this follow-up, IL-6 and Bax expression levels in the placenta, together with histopathological findings and serum CRP levels, can guide the evaluation of the prognosis, severity and response to treatment of the disease.

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Intestinal morphology and mucin composition in Japanese quails fed on olive cake

Karakoç¹,

Özcan²

2023

SA J. An. Sci.

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Olive cake, sometimes also called spent olive, is a low-energy nutrition source. It possesses various biological properties that are antioxidant, anti-inflammatory, antimicrobial, and antiviral in property due to its rich polyphenolic compound content. Mucins can be found in the composition of the mucus that cover the surface of the gastrointestinal tract. Feed additives can influence the mucin composition, as well as the height and width of the villi, which are biological appendages tasked to increase the absorption surface in the small intestinal mucosa. The aim of the present study was to determine the histology of the small intestine in Japanese quails fed with olive cake. In the study, mixed-sex quails fed with rations containing different amounts of olive cake were used as the live material. Morphological measurements, Alcian blue/periodic acid–Schiff, and aldehyde fuchsin/Alcian blue histochemical stains were performed on stomach and small intestine tissues taken from quails. Significant increases in villus height, villus width, and crypt depth in duodenum, jejunum, and ileal tissues were determined in control and experimental groups. The increase in crypt depth was greatest in the ileum. In the jejunal tissue, it was determined that the width of the villus decreased in groups 1 and 2 but increased in group 3. In the histochemical evaluation, it was determined that sulphate mucins were intense in the proventriculus, while carboxylic mucins were intense in all three parts of the small intestine. Considering the physiological functions of mucins, olive cake is thought to play an important role in the protection of the mucosa in quails.

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Kıl keçisi abomasumunda ghrelin, obestatin ve leptin hormonlarının immunohistokimyasal dağılımları

Cited by 3 publications

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Immunohistochemical distributions of leptin, ghrelin and obestatin hormones in bull and ram abomasum

Immunohistochemical distributions of leptin, ghrelin and obestatin hormones in bull and ram abomasum

Histopathological examination of the placenta after delivery in pregnant women with COVID-19

Intestinal morphology and mucin composition in Japanese quails fed on olive cake

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