Killing and Mutation of Chinese Hamster V79 Cells Exposed to Accelerated Oxygen and Neon Ions

Abstract

Mutation induction by accelerated heavy ions to 6-thioguanine resistance (HPRT system) in Chinese hamster V79 cells was investigated using oxygen and neon ions with energies between 1.9 and 400 MeV/mu, corresponding to LET values between 18 and 754 keV/microns, respectively. Because of technical limitations most experiments could be performed only once. Inactivation and mutation induction cross sections, sigma i and sigma m, were obtained from the slopes of the exponential survival and the linear mutation induction curves, respectively. Both parameters increased with LET up to about 200 keV/microns, where the curves separated for the two types of ions. Calculated RBEs were higher for mutation induction than for killing for all LET values.

Applications of amorphous track models in radiation biology

“…Comparisons of calculation [51] to experiments [43][44][45][46] for HPRT mutation in V79 cells are shown in Fig. 3b.…”

“…In Fig. 3a, calculations with the IGK model are compared to data [43][44][45][46] for V79 inactivation. The parameters used for inactivation of V79 cells are shown where m=3, D 0 =1.8 Gy, σ 0 =43 µm 2 , and a 0 =1.1 µm.…”

“…3b. The data are from Belli et al [43] for protons and helium ions, Thacker et al [44] for B ions, and Kiefer and co-workers [45,46] for other heavy ions. A prediction of the model is that a depletion of the mutation cross-section occurs at energies where the inactivation cross-section is a maximum.…”

The Influence of Mutation Induction in Normal Human Fibroblasts Irradiated with X Rays and Iron Ions

“…Several researchers reported that the maximum RBE values in LET-RBE curves for mutation induction with around 100 -200 keV/µm were higher than these of other biological endpoints such as cell killing, but the RBE values in the high LET regions over 200 keV/µm were lower than cell killing in previous repots (Tsuboi et al, 1992;Chen et al, 1994;Stoll et al, 1995;Suzuki et al, 1996;Schmidt et al, 1998;Kiefer et al, 2001). The present data of this study showed the same tendency when compared to our data for cell killing using the same culture system (Tsuruoka et al, 2005).…”

“…It is well known that high-LET radiation such as heavy ions is more effective than low-LET radiation such as X and gamma rays for various biological effects such as the cell-killing effect, transformation, chromosome aberration and mutation induction (Skarsgard et al, 1967;Yang et al, 1985;Hei et al, 1988;Alpen et al, 1993;Alpen et al, 1994;Suzuki et al, 2000;Kiefer et al, 2001;Tsuruoka et al, 2005;Suzuki et al, 2006). The RBE values of maximum efficiency for mutation induction were higher than other biological endpoints such as cell killing (Tsuboi et al, 1992;Chen et al, 1994;Stoll et al, 1995;Suzuki et al, 1996;Schmidt et al, 1998;Kiefer et al, 2001). Furthermore, high-LET radiations were reported to be more cytotoxic and genotoxic than low-LET radiations (Blakely and Kronenberg, 1998;Cucinotta and Durante, 2006).…”