Killing cancer cells by delivering a nanoreactor for inhibition of catalase and catalytically enhancing intracellular levels of ROS

Kankala, Ranjith Kumar; Kuthati, Yaswanth; Liu, Chen‐Lun; Mou, Chung‐Yuan; Lee, Chia‐Hung

doi:10.1039/c5ra16023e

Cited by 60 publications

(78 citation statements)

References 76 publications

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“…The surface area and pore volume were sharply reduced to 29 m 2 /g and 0.22 cm 3 /g, respectively, which provided further supportive evidence of HTCC coating. Lee has reported that the BET surface area was dramatically reduced from 1198 m 2 /g to 20 m 2 /g after heavy aminotriazole loading of copper-impregnated MSNs [31]. Additionally, the loss of the hysteresis loop affirms that the pores were blocked by the polymer coating and lost their mesoporous structure characteristics.…”

Section: Resultsmentioning

confidence: 98%

“…The 1088 cm −1 broad absorption band was ascribed to Si–O–Si (siloxane) strething vibrations. Almost no C–H stretch peaks are present in the MSN samples, implying that the surfactant template of CTAB was completely extracted [31]. The peak at 1481 cm −1 for the HTCC spectra can be assigned to the C–H bending vibration of –N(CH 3 ) 3 + , confirming the existence of a quaternized amine group in the CS backbone [32].…”

Section: Resultsmentioning

confidence: 99%

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Quaternized Chitosan-Capped Mesoporous Silica Nanoparticles as Nanocarriers for Controlled Pesticide Release

et al. 2016

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Nanotechnology-based pesticide formulations would ensure effective utilization of agricultural inputs. In the present work, mesoporous silica nanoparticles (MSNs) with particle diameters of ~110 nm and pore sizes of ~3.7 nm were synthesized via a liquid crystal templating mechanism. A water-soluble chitosan (CS) derivative (N-(2-hydroxyl)propyl-3-trimethyl ammonium CS chloride, HTCC) was successfully capped on the surface of pyraclostrobin-loaded MSNs. The physicochemical and structural analyses showed that the electrostatic interactions and hydrogen bonding were the major forces responsible for the formation of HTCC-capped MSNs. HTCC coating greatly improved the loading efficiency (LC) (to 40.3%) compared to using bare MSNs as a single encapsulant (26.7%). The microstructure of the nanoparticles was revealed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The pyraclostrobin-loaded nanoparticles showed an initial burst and subsequent sustained release behavior. HTCC-capped MSNs released faster than bare MSNs in the initial stage. Pyraclostrobin-loaded HTCC-capped MSNs with half doses of pyraclostrobin technical demonstrated almost the same fungicidal activity against Phomopsis asparagi (Sacc.), which obviously reduced the applied pesticide and enhanced the utilization efficiency. Therefore, HTCC-decorated MSNs demonstrated great potential as nanocarriers in agrochemical applications.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Quaternized Chitosan-Capped Mesoporous Silica Nanoparticles as Nanocarriers for Controlled Pesticide Release

et al. 2016

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“…The expressions of respective vascular protein markers were investigated using Western blot method. The proteins were extracted by following the reported procedure . The tissue block was cleaved, and the cells were collected and homogenized using the radioimmunoprecipitation assay (RIPA) buffer.…”

Section: Methodsmentioning

confidence: 99%

Highly Porous Microcarriers for Minimally Invasive In Situ Skeletal Muscle Cell Delivery

Kankala

Zhao

Liu

et al. 2019

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Microscale cell carriers have recently garnered enormous interest in repairing tissue defects by avoiding substantial open surgeries using implants for tissue regeneration. In this study, the highly open porous microspheres (HOPMs) are fabricated using a microfluidic technique for harboring proliferating skeletal myoblasts and evaluating their feasibility toward cell delivery application in situ. These biocompatible HOPMs with particle sizes of 280–370 µm possess open pores of 10–80 µm and interconnected paths. Such structure of the HOPMs conveniently provide a favorable microenvironment, where the cells are closely arranged in elongated shapes with the deposited extracellular matrix, facilitating cell adhesion and proliferation, as well as augmented myogenic differentiation. Furthermore, in vivo results in mice confirm improved cell retention and vascularization, as well as partial myoblast differentiation. These modular cell‐laden microcarriers potentially allow for in situ tissue construction after minimally invasive delivery providing a convenient means for regeneration medicine.

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“…Furthermore, when used in combination with copper, DSF becomes a strong inducer of radical oxygen species (ROS) [27][28][29][30]. Despite its powerful anticancer activity, the development of DSFbased cancer therapy has been hampered by its poor solubility in biological fluid and instability in gastric juice and bloodstream [31].…”

Section: Introductionmentioning

confidence: 99%

Pancreatic cancer stem cell proliferation is strongly inhibited by diethyldithiocarbamate-copper complex loaded into hyaluronic acid decorated liposomes

Marengo

Forciniti

Dando

et al. 2019

Biochimica et Biophysica Acta (BBA) - General Subjects

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Background: Pancreatic cancer stem cells (CSCs) are responsible for resistance to standard therapy, metastatic potential, and disease relapse following treatments. The current therapy for pancreatic ductal adenocarcinoma (PDAC) preferentially targets the more differentiated cancer cell population, leaving CSCs as a cell source for tumor mass formation and recurrence. For this reason, there is an urgent need to improve current therapies and develop novel CSC-targeted therapeutic approaches. Methods: Hyaluronic acid (HA) decorated liposomes, containing diethyldithiocarbamate-copper (Cu(DDC) 2), able to target the specific CSC marker CD44 receptor were prepared by ion gradient technique and fully characterized. Their antiproliferative effect was evaluated on pancreatic CSCs derived from PDAC cell lines or patients. To clarify the mechanism of action of Cu(DDC) 2 liposomes, ROS level neutralization assay in the presence of N-acetyl-L-cysteine was performed. Results: Liposomes showed high encapsulation efficiency and Cryo-TEM analysis revealed the presence of Cu(DDC) 2 crystals in the aqueous core of liposomes. In vitro test on pancreatic CSCs derived from PDAC cell lines or patients showed high ROS mediated anticancer activity of HA decorated liposomes. The sphere formation capability of CSCs obtained from patients was drastically reduced by liposomal formulations containing Cu(DDC) 2. Conclusions: The obtained results show that the encapsulation of Cu(DDC) 2 complex in HA decorated liposomes strongly increases its anti-proliferative activity on pancreatic CSCs. General significance: This paper describes for the first time the use of HA decorated liposomes containing Cu(DDC) 2 against pancreatic CSCs and opens the way to the development of nanomedicine based CSC-targeted therapeutic approaches.

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Killing cancer cells by delivering a nanoreactor for inhibition of catalase and catalytically enhancing intracellular levels of ROS

Abstract: Intracellular hydrogen peroxide levels have the potential to be exploited in cancer therapy.

Cited by 60 publications

References 76 publications

Quaternized Chitosan-Capped Mesoporous Silica Nanoparticles as Nanocarriers for Controlled Pesticide Release

Quaternized Chitosan-Capped Mesoporous Silica Nanoparticles as Nanocarriers for Controlled Pesticide Release

Highly Porous Microcarriers for Minimally Invasive In Situ Skeletal Muscle Cell Delivery

Pancreatic cancer stem cell proliferation is strongly inhibited by diethyldithiocarbamate-copper complex loaded into hyaluronic acid decorated liposomes

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