Killing of Gram-Negative Bacteria by Neutrophils: Role of O2- Independent System in Intracellular Killing and Evidence of O2-Dependent Extracellular Killing

Weiss, Jerrold; Victor, Michael; Kao, L; Elsbach, Peter

doi:10.1007/978-1-4684-8326-0_3

Cited by 3 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cross-linking of FcaRI by polymeric IgA on neutrophils has been shown to induce respiratory burst leading to bacterial killing (Shen 1992, Weiss et al 1985. This is also likely to occur upon binding of IgA-opsonized bacterial aggregates in periodontal pockets.…”

Section: Discussionmentioning

confidence: 99%

Fcα receptor I (CD89) on neutrophils in periodontal lesions

Yuan¹,

Gjermo²,

Helgeland³

et al. 2000

J Clinic Periodontology

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Fcα receptor I (CD89) on neutrophils in periodontal lesions

Yuan¹,

Gjermo²,

Helgeland³

et al. 2000

J Clinic Periodontology

View full text Add to dashboard Cite

show abstract

“…This action may be aa;ounted for by the high affinity of the very basic N-terminal half of the molecule for the negatively charged LPS moieties that are uniquely found in the outer envelope of Gramnegative bacteria [13][14][15]25], and which constitute an important part of free endotoxin when it is released from the bacterial cell wall, hence the reported action of BPI as an endotoxin neutralizing protein [26]. In 1982 and 1985 Weiss et ai found that the bactericidal activity of crude acid extract of human neutrophils toward susceptible Echerichia coli corresponded closely to the BFI content, and was completely blocked by a goat anti-BPI IgG-rich fraction, but not by the preimmune IgG-rich fraction [27,28]. These results on the one hand suggested that BPI was an important cytotoxic protein of the PMN, and on the other hand Purified BPI (lO^tg/Iane) was run in SDS-PAGE undernon-reducing conditions and transferred on to a nitrocellulose filter.…”

Section: Discussionmentioning

confidence: 99%

Bactericidal/permeability-increasing protein (BPI) is an important antigen for anti-neutrophil cytoplasmic autoantibodies (ANCA) in vasculitis

Zhao

Jones

Lockwood

1995

Clinical and Experimental Immunology

143

View full text Add to dashboard Cite

SUMMARYIndirect immunofluorescence (IIF) techniques have shown that ANCA are useful serological markers for some small vessel vasculitides, and ELISA assays, using purified molecules as solidphase ligand, have helped to identify proteinase 3 (PR3) and myeloperoxidase (MPO) as two of the major ANCA antigens. There remain a substantial number of serum samples, which are positive by IIF, yet recognize neither PR3 nor MPO (double-negative samples). We found, by Western blot analysis of soluble neutrophil granule proteins, that certain ofthese double-negative samples recognized a 55-kD doublet of which the first eight residues shared N-terminal amino acid sequence homology with BPI, a potent antibiotic towards Gram-negative bacteria. We developed a simple, quick and robust two-step immunobiochemical method to purify BPI. This was then employed to detect anti-BPI autoantibodies by ELISA and Western blot analysis. We tested 100 double-negative samples and 400 consecutive new samples sent for routine ANCA testing in the anti-BPI ELISA. We found that 45 of the 100 double-negative and 44 of the 400 new routine samples recognized BPI. By Western blot analysis 20/20 positive anti-BPI samples blotted the 55-kD protein. Inhibition assays confirmed the specificity of binding. Review of the 89 anti-BPI-positive patients showed a male dominance (M: F ratio 55:34), a mean age of 60 4 years and clinical diagnoses ranging from organ limited vasculitis to widespread systemic vasculitis.

show abstract

Seroprevalence and disease association of antineutrophil cytoplasmic autoantibodies and antigens in HIV infection

et al. 1999

View full text Add to dashboard Cite

This prospective study was designed to determine the role of antineutrophil cytoplasmic autoantibodies (ANCA) in HIV-infected patients. Immunofluorescence tests (IFT) and enzyme-linked immunosorbent assays (ELISA) were applied to sera of 199 consecutive outpatients. In the IFT 20% were positive. An atypical ANCA pattern was demonstrated in 67% of these, 33% revealed a perinuclear staining (pANCA). Specific ELISA revealed proteinase 3 (n = 2), myeloperoxidase (n = 1), lysozyme (n = 2), lactoferrin (n = 1), cathepsin G (n = 1), and human leukocyte elastase (HLE, n = 6). The target antigen remained unidentified in 26 patients. Perinuclear ANCA-positive patients showed atypical antigens in eight of 13 cases; all six patients with anti-HLE revealed a pANCA pattern. The antigens of atypical ANCA-positive patients remained unidentified in 21 of 26 (81%) cases. No signs of vasculitis were present in the ANCA-positive patients. ANCA are frequently found in the sera of HIV-positive patients. They bind to a variety of antigens. No correlation was found between ANCA positivity and autoimmune or opportunistic diseases.

show abstract

Killing of Gram-Negative Bacteria by Neutrophils: Role of O2- Independent System in Intracellular Killing and Evidence of O2-Dependent Extracellular Killing

Cited by 3 publications

References 16 publications

Fcα receptor I (CD89) on neutrophils in periodontal lesions

Fcα receptor I (CD89) on neutrophils in periodontal lesions

Bactericidal/permeability-increasing protein (BPI) is an important antigen for anti-neutrophil cytoplasmic autoantibodies (ANCA) in vasculitis

Seroprevalence and disease association of antineutrophil cytoplasmic autoantibodies and antigens in HIV infection

Contact Info

Product

Resources

About