Kilohertz retinal FF-SS-OCT and flood imaging with hardware-based adaptive optics

Valente, Denise; Vienola, Kari V.; Zawadzki, Robert J.; Jonnal, Ravi S.

doi:10.1101/2020.07.23.218594

Cited by 2 publications

(3 citation statements)

References 41 publications

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“…Each 2D image is then transformed into an OCT B-scan, as would a series of spectral measurements along the fast scan in a traditional system. Their approach yields B-scan rates higher than traditional scanning systems-up to 16 kHz, corresponding to 8 MHz A-scan rates-but lower than the effective A-scan rates of FF-SS-OCT systems (111,126). With this system, they were able to measure light-evoked deformations of the cone OS, and use these to spectrally classify cones.…”

Section: Phase-sensitive Adaptive Optics Oct (Ao-oct)mentioning

confidence: 99%

“…To overcome the computational demands of highorder DAC, our group developed an FF-SS-OCT system with hardware AO (126). The system was demonstrated to acquire ~ 1° retinal volumes at 200 Hz, sufficient for measuring both the initial contractile phase and elongation of cones, with the potential lateral resolution to measure rod changes as well.…”

Section: Phase-sensitive Adaptive Optics Oct (Ao-oct)mentioning

confidence: 99%

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Toward a clinical optoretinogram: a review of noninvasive, optical tests of retinal neural function

Jonnal¹

2021

Ann Transl Med

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The past few years have witnessed rapid development of the optoretinogram-a noninvasive, optical measurement of neural function in the retina, and especially the photoreceptors (Ph). While its recent development has been rapid, it represents the culmination of hundreds of experiments spanning decades.Early work showed measurable and reproducible changes in the optical properties of retinal explants and suspensions of Ph, and uncovered some of the biophysical and biochemical mechanisms underlying them.That work thus provided critical motivation for more recent work based on clinical imaging platforms, whose eventual goal is the improvement of ophthalmic care and streamlining the discovery of novel therapeutics.The first part of this review consists of a selective summary of the early work, and identifies four kinds of stimulus-evoked optical signals that have emerged from it: changes in light scattered from the membranous discs of the Ph's outer segment (OS), changes in light scattered by the front and back boundaries of the OS, rearrangement of scattering material in and near the OS, and changes in the OS length. In the past decade, all four of these signals have continued to be investigated using imaging systems already used in the clinic or intended for clinical and translational use. The second part of this review discusses these imaging modalities, their potential to detect and quantify the signals of interest, and other factors influencing their translational promise. Particular attention is paid to phase-sensitive optical coherence tomography (OCT) with adaptive optics (AO), a method in which both the amplitude and the phase of light reflected from individual Ph is monitored as visible stimuli are delivered to them. The record of the light's phase is decoded to reveal a reproducible pattern of deformation in the OS, while the amplitude reveals changes in scattering and structural rearrangements. The method has been demonstrated in a few labs and has been used to measure responses from both rods and cones. With the ability to detect responses to stimuli isomerizing less than 0.01% of photopigment, this technique may prove to be a quick, noninvasive, and objective way to measure subtle disease-related dysfunction at the cellular level, and thus to provide an entirely new and complementary biomarker for retinal disease and recovery.

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Section: Phase-sensitive Adaptive Optics Oct (Ao-oct)mentioning

confidence: 99%

Section: Phase-sensitive Adaptive Optics Oct (Ao-oct)mentioning

confidence: 99%

Toward a clinical optoretinogram: a review of noninvasive, optical tests of retinal neural function

Jonnal¹

2021

Ann Transl Med

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“…Hardware adaptive optics (HAO) OCT utilizes a deformable mirror to compensate for wavefront aberrations. Aberration-free images of nerve fibers and photoreceptors in the human and animal retina have been acquired in-vivo using this approach [17,18]. However, HAO-OCT systems are associated with significantly higher cost, complex and bulky optical design, and difficulty with the optimal optical alignment.…”

Section: Introductionmentioning

confidence: 99%

Correcting for Spatial-Spectral Crosstalk and Chromatic Aberrations in Broadband Line-Scan Spectral-Domain OCT images

Han¹,

Bizheva²

2023

Preprint

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Digital correction of optical aberrations allows for high-resolution imaging across the full depth range in optical coherence tomography (OCT). Many digital aberration correction (DAC) methods have been proposed in the past to evaluate and correct monochromatic wavefront phase errors in OCT images. However, other factors that deteriorate the image quality have not been fully investigated. Here we present a novel approach to digital recovery of the spatial resolution in images acquired with a broadband Line-Scan Spectral-Domain OCT system (LS-SD-OCT), which addresses 2main factors that limit the effectiveness of DAC for restoring diffraction-limited resolution in LS-SD-OCT images: spatial-spectral crosstalk and chromatic aberrations. In the proposed approach, spatial-spectral crosstalk and chromatic aberrations were effectively suppressed by registration of monochromatic sub-band tomograms that were digitally corrected for aberrations. The new method was validated by imaging a standard resolution target, a microspheres phantom, and different biological tissues. LS SD-OCT technology combined with the proposed novel image reconstruction method could be a valuable research tool for various biomedical and clinical applications.

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Kilohertz retinal FF-SS-OCT and flood imaging with hardware-based adaptive optics

Cited by 2 publications

References 41 publications

Toward a clinical optoretinogram: a review of noninvasive, optical tests of retinal neural function

Toward a clinical optoretinogram: a review of noninvasive, optical tests of retinal neural function

Correcting for Spatial-Spectral Crosstalk and Chromatic Aberrations in Broadband Line-Scan Spectral-Domain OCT images

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