Kimchi markedly induces apoptosis in HT‐29 human colon carcinoma cells

Yu, Ting; Park, Eui-Seong; Song, Gil-Hoon; Yi, Ruokun; Park, Kun-Young

doi:10.1111/jfbc.13532

Cited by 17 publications

(8 citation statements)

References 38 publications

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“…RNA was isolated from colon tissue of each group using TRIzol reagent (Invitrogen Co., Carlsbad, CA, USA), and a NanoDrop ND-1000 (NanoDrop Technologies Inc., Wilmington, DE, USA) was used to quantify the isolated RNA. Afterwards, the quanti ed RNA was reverse transcribed using Superscript II reverse transcriptase (Invitrogen Co.), synthesized into cDNA, and used in experiments [21]. This cDNA was mixed with related primers (Bioneer, Daejeon, Korea) and SYBR green (Solis BioDyne, Tartu, Estonia), and gene expression was analyzed using a thermal cycler Bio-Rad CFX-96 real time system (Bio-Rad, Hercules, CA, USA) [22].…”

Section: Rt-qpcr Analysismentioning

confidence: 99%

Anticancer Effects of Washed-Dehydrated Solar Salt Doenjang and its Metabolites

Lee,

Hong,

Park

2023

Preprint

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In this study, the anticancer effect of doenjang according to the type of salt was investigated. Three samples were prepared: doenjang made with purified salt (PSD), doenjang made with generally manufactured solar salt (GSD), and doenjang made with washed and dehydrated solar salt (WDSD). In mice in which colon cancer was induced with azoxymethane (AOM)/dextran sodium sulfate (DSS), doenjang made with solar salt, especially doenjang made with washed and dehydrated solar salt, was found to have a much higher colon cancer inhibition effect. WDSD significantly promoted the mRNA expression of apoptosis-related factors such as Bcl-2–associated X protein (Bax) and caspase 9 and the cell cycle arrest-related factors p53 and p21, and conversely significantly reduced the mRNA expression of apoptosis inhibitors such as B-cell lymphoma-2 (Bcl-2) (p < 0.05). Additionally, metabolites were investigated to determine which substances in WDSD exhibit this anticancer effect. As a result, the contents of isoflavone and soyasaponin B in the form of aglycons such as genistein, daidzein, and glycitein, which are known to have anticancer and anti-inflammatory properties, were found to be significantly high. Therefore, the results confirmed that doenjang prepared with washed and dehydrated solar salt has superior anticancer potential against colon cancer, and that various active ingredients contribute to the improvement of this functionality.

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Section: Rt-qpcr Analysismentioning

confidence: 99%

Anticancer Effects of Washed-Dehydrated Solar Salt Doenjang and its Metabolites

Lee,

Hong,

Park

2023

Preprint

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“…The optical density (OD) value of each well was measured at 490 nm, and the inhibition rate of cell proliferation was calculated. The proliferation inhibition rate (%) = (OD control well -OD sample well )/OD control well × 100 [14].…”

Section: Detection Of Cell Proliferationmentioning

confidence: 99%

Polyphenols in Ilex latifolia Thunb. inhibit human lung cancer cell line A549 by regulation of the PI3K-Akt signaling pathway

Chen

Long

et al. 2022

BMC Complement Med Ther

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Background The leaves of the plant Ilex latifolia Thunb. can be made into Kuding tea, which is a drink rich in polyphenols. This study aimed to observe the effect of Ilex latifolia Thunb. polyphenols (ILTPs) on human lung cancer cell line A549 (A549 cells) by regulating the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway. Methods In vitro cultured cells were treated with ILTPs; the proliferation of A549 cells and BEAS-2B human normal lung epithelial cells (Beas-2B cells) was observed using the 3-(4,5-dimethylazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the survival status of A549 cells was observed by fluorescence staining. The expression of A549 cells was observed by quantitative polymerase chain reaction (qPCR) assay and Western blot analysis, while the compound composition of ILTPs was detected using high-performance liquid chromatography (HPLC). Results The experimental results showed that the proliferation of Beas-2B cells was unaffected by treatment with 0–500 μg/mL of ILTPs, whereas the decreased proliferation of A549 cells was observed with the increasing concentrations of ILTPs. Additionally, ILTPs elevated the levels of lactate dehydrogenase (LDH) and reactive oxygen species (ROS) and promoted apoptosis in A549 cells. The results of qPCR experiments showed that ILTPs upregulated caspase-9 mRNA expression and downregulated phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), mammalian target of rapamycin (mTOR), B-cell lymphoma-2 (Bcl-2), nuclear factor-κB (NF-κB), vascular endothelial growth factor (VEGF), hypoxia-inducible factor-1 alpha (HIF-1α), and cyclooxygenase-2 (COX-2) expression in A549 cells. The Western blot analysis results also showed that ILTPs could reduce the protein expression of PI3K and Akt. The HPLC results showed that the main compounds present in the ILTPs were rutin, kaempferol, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C. Conclusions Thus, this study indicated that the polyphenols of I. latifolia act as a class of natural functional food materials that potently suppress cancer by exerting their inhibitory effects on A549 cell proliferation through five key polyphenolic compounds.

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“…Depending on whether the ingredients are added or whether they are organic or non-organic, the taste, flavor, and textural and functional properties, such as anticancer and anti-inflammatory effects, may change [3]. In particular, in the study of Yu et al [4], when only organic cabbage was used, the proliferation of HT-29 cells was significantly inhibited through the activation of apoptosis, and the addition of sub-ingredients confirmed that the apoptosis effect improved.…”

Section: Introductionmentioning

confidence: 99%

Increased Anticancer Activity of Organic Kimchi with Starters Demonstrated in HT-29 Cancer Cells

et al. 2023

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This study aims to investigate the effects of kimchi made with organic ingredients and lactic acid bacteria (LAB) as starters (Leuconostoc mesenteroides + Lactiplantibacillus plantarum) on HT-29 human colon carcinoma cells. Four types of kimchi (standard kimchi (SK), commercial kimchi (CK), anticancer kimchi (AK), and organic anticancer kimchi (OAK)) were evaluated. The results show that, among the different types of kimchi studied, OAK presents high DPPH free-radical scavenging activity and total phenol and flavonoid contents, and the MTT assay shows that the growth inhibition rate against HT-29 cancer cells is the highest. In addition, the quantitative reverse transcription polymerase chain reaction (RT-qPCR) results show that, compared to SK and CK, AK and OAK can effectively down-regulate the mRNA expression of anti-apoptotic gene Bcl-2 and up-regulate the mRNA expression of the cell cycle arrest genes p21 and p53; pro-apoptotic genes Bim, Bak, and Bad; and genes for caspases 3 9. Subsequently, a Western blot test confirmed that the expression of Bcl-2 decreased and the expressions of p53, Bax, and caspases 3 and 9 increased in OAK. The abovementioned results indicate that the anticancer kimchi prepared with organic ingredients and starters of lactic acid bacteria effectively present the best antioxidant activity and inhibit the proliferation of HT-29 cancer cells by promoting apoptosis and cell cycle arrest.

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Kimchi markedly induces apoptosis in HT‐29 human colon carcinoma cells

Cited by 17 publications

References 38 publications

Anticancer Effects of Washed-Dehydrated Solar Salt Doenjang and its Metabolites

Anticancer Effects of Washed-Dehydrated Solar Salt Doenjang and its Metabolites

Polyphenols in Ilex latifolia Thunb. inhibit human lung cancer cell line A549 by regulation of the PI3K-Akt signaling pathway

Increased Anticancer Activity of Organic Kimchi with Starters Demonstrated in HT-29 Cancer Cells

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