Kin3 protein, a NIMA-related kinase of<i>Saccharomyces cerevisiae</i>, is involved in DNA adduct damage response

Moura, Dinara Jaqueline; Castilhos, Bruna; Immich, Bruna Frielink; Cañedo, Andrés Delgado; Henriques, João Antônio Pêgas; Lenz, Guido; Saffi, Jenifer

doi:10.4161/cc.9.11.11892

Cited by 13 publications

(10 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A kin3 Δ strain was shown to be sensitive to a variety of DNA-damaging agents, suggesting a role for Kin3 in DNA damage repair (Moura et al 2010). Studies on the Aspergillus nidulans ortholog of Kin3 show a functional interaction with microtubules and the Endosomal Sorting Complex Required for Transport (ESCRT) pathway (Govindaraghavan et al 2014).…”

Section: Resultsmentioning

confidence: 99%

Identification of Genes inSaccharomyces cerevisiaethat Are Haploinsufficient for Overcoming Amino Acid Starvation

Bae

Seberg

Carroll

et al. 2017

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

The yeast Saccharomyces cerevisiae responds to amino acid deprivation by activating a pathway conserved in eukaryotes to overcome the starvation stress. We have screened the entire yeast heterozygous deletion collection to identify strains haploinsufficient for growth in the presence of sulfometuron methyl, which causes starvation for isoleucine and valine. We have discovered that cells devoid of MET15 are sensitive to sulfometuron methyl, and loss of heterozygosity at the MET15 locus can complicate screening the heterozygous deletion collection. We identified 138 cases of loss of heterozygosity in this screen. After eliminating the issues of the MET15 loss of heterozygosity, strains isolated from the collection were retested on sulfometuron methyl. To determine the general effect of the mutations for a starvation response, SMM-sensitive strains were tested for the ability to grow in the presence of canavanine, which induces arginine starvation, and strains that were MET15 were also tested for growth in the presence of ethionine, which causes methionine starvation. Many of the genes identified in our study were not previously identified as starvation-responsive genes, including a number of essential genes that are not easily screened in a systematic way. The genes identified span a broad range of biological functions, including many involved in some level of gene expression. Several unnamed proteins have also been identified, giving a clue as to possible functions of the encoded proteins.

show abstract

Section: Resultsmentioning

confidence: 99%

Identification of Genes inSaccharomyces cerevisiaethat Are Haploinsufficient for Overcoming Amino Acid Starvation

Bae

Seberg

Carroll

et al. 2017

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

show abstract

“…BUD14 is involved in bud-site selection [26], ADE1 is involved in de novo purine biosynthesis [27], KIN3 encodes a non-essential serine/threonine protein kinase involved in a.o. DNA damage repair [28] and CDC15 encodes a protein kinase involved in control of the cell division cycle [29]. In order to identify the genes(s) involved in ethanol accumulation capacity, we investigated the most likely candidate genes individually with the classical one-gene RHA [10].…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the superior KIN3 allele of Seg5 contained two SNPs, which were absent in the KIN3 gene of all yeast strains of which the genome has been fully sequenced up to now, suggesting that they may be important for the exceptional ethanol accumulation capacity of the Seg5 strain and its diploid parent CBS1585. KIN3 encodes a serine-threonine protein kinase, required for arrest at the G2/M-phase checkpoint in response to the DNA damage inducing agents MMS, cisplatin, doxorubicin and nitrogen mustard [28]. Involvement of Kin3 in the DNA damage response may be consistent with its requirement for tolerance to high ethanol levels.…”

Section: Discussionmentioning

confidence: 99%

Comparative Polygenic Analysis of Maximal Ethanol Accumulation Capacity and Tolerance to High Ethanol Levels of Cell Proliferation in Yeast

et al. 2013

View full text Add to dashboard Cite

The yeast Saccharomyces cerevisiae is able to accumulate ≥17% ethanol (v/v) by fermentation in the absence of cell proliferation. The genetic basis of this unique capacity is unknown. Up to now, all research has focused on tolerance of yeast cell proliferation to high ethanol levels. Comparison of maximal ethanol accumulation capacity and ethanol tolerance of cell proliferation in 68 yeast strains showed a poor correlation, but higher ethanol tolerance of cell proliferation clearly increased the likelihood of superior maximal ethanol accumulation capacity. We have applied pooled-segregant whole-genome sequence analysis to identify the polygenic basis of these two complex traits using segregants from a cross of a haploid derivative of the sake strain CBS1585 and the lab strain BY. From a total of 301 segregants, 22 superior segregants accumulating ≥17% ethanol in small-scale fermentations and 32 superior segregants growing in the presence of 18% ethanol, were separately pooled and sequenced. Plotting SNP variant frequency against chromosomal position revealed eleven and eight Quantitative Trait Loci (QTLs) for the two traits, respectively, and showed that the genetic basis of the two traits is partially different. Fine-mapping and Reciprocal Hemizygosity Analysis identified ADE1, URA3, and KIN3, encoding a protein kinase involved in DNA damage repair, as specific causative genes for maximal ethanol accumulation capacity. These genes, as well as the previously identified MKT1 gene, were not linked in this genetic background to tolerance of cell proliferation to high ethanol levels. The superior KIN3 allele contained two SNPs, which are absent in all yeast strains sequenced up to now. This work provides the first insight in the genetic basis of maximal ethanol accumulation capacity in yeast and reveals for the first time the importance of DNA damage repair in yeast ethanol tolerance.

show abstract

“…Note that we considered both S. pombe orthologs of HOF1, namely, cdc15 and imp2 in our analysis. These genes are followed by KIN3, G2/M check point gene whose protein Kin3 plays a critical role in DNA damage recognition before the cell enters M phase ( 18 ). Among the M phase genes in the proposed relative order, DBF2 and CDC20 have a function for cells to exit from mitosis, while PST1 has function in the construction of cell wall.…”

Section: Methodsmentioning

confidence: 99%

Identification of a core set of signature cell cycle genes whose relative order of time to peak expression is conserved across species

Fernández¹,

Rueda²,

Peddada³

2011

Nucleic Acids Research

View full text Add to dashboard Cite

A cell division cycle is a well-coordinated process in eukaryotes with cell cycle genes exhibiting a periodic expression over time. There is considerable interest among cell biologists to determine genes that are periodic in multiple organisms and whether such genes are also evolutionarily conserved in their relative order of time to peak expression. Interestingly, periodicity is not well-conserved evolutionarily. A conservative estimate of a number of periodic genes common to fission yeast (Schizosaccharomyces pombe) and budding yeast (Saccharomyces cerevisiae) (‘core set FB’) is 35, while those common to fission yeast and humans (Homo sapiens) (‘core set FH’) is 24. Using a novel statistical methodology, we discover that the relative order of peak expression is conserved in ∼80% of FB genes and in ∼40% of FH genes. We also discover that the order is evolutionarily conserved in six genes which are potentially the core set of signature cell cycle genes. These include ace2 (a transcription factor) and polo-kinase plo1, which are well-known hubs of early M-phase clusters, cdc18 a key component of pre-replication complexes, mik1 which is critical for the establishment and maintenance of DNA damage check point, and histones hhf1 and hta2.

show abstract

Kin3 protein, a NIMA-related kinase ofSaccharomyces cerevisiae, is involved in DNA adduct damage response

Cited by 13 publications

References 62 publications

Identification of Genes inSaccharomyces cerevisiaethat Are Haploinsufficient for Overcoming Amino Acid Starvation

Identification of Genes inSaccharomyces cerevisiaethat Are Haploinsufficient for Overcoming Amino Acid Starvation

Comparative Polygenic Analysis of Maximal Ethanol Accumulation Capacity and Tolerance to High Ethanol Levels of Cell Proliferation in Yeast

Identification of a core set of signature cell cycle genes whose relative order of time to peak expression is conserved across species

Contact Info

Product

Resources

About