Kinetic analyses of transmitter release in neuromuscular transmission

Maéno, Takashi; Enomoto, Koh-ichi

doi:10.1016/0065-227x(88)90005-6

Cited by 5 publications

(1 citation statement)

References 78 publications

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“…It is generally accepted that acetylcholine (ACh) in nerve terminals is mostly stored in packets, morphologically corresponding to a number of vesicles. ACh is released in a quanta1 manner either spontaneously or synchronously with the arrival of nerve impulses, and ACh is removed by both enzymic hydrolysis and free diffusion (Katz & Miledi 1973;Magleby & Terrar 1975;Maeno & Enomoto 1988). The hydrolysis by cholinesterase (ChE) is very effective and rapid.…”

Section: (A)mentioning

confidence: 99%

A Possible Interpretation for Difference in Neostigmine‐induced Changes of Spontaneous Activities and Evoked Muscle Potentials Between Rat Medial Gastrocnemius and Soleus Muscles

Uramoto

Watanabe

Totsuka

1993

Clin Exp Pharma Physio

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1. Under urethane-anaesthesia, changes of spontaneous activities and evoked muscle potentials in rat medial gastrocnemius (MG) and soleus (SOL) muscles induced by the injection of neostigmine were investigated. 2. Before the administration of neostigmine, spontaneous activities were rarely observed and muscle potentials evoked by single shocks were simply biphasic in both MG and SOL muscles. When the anti-cholinesterase drug was applied, spontaneous discharges of motor units were often observed in bursts in the MG muscles, in contrast to single spikes in the SOL muscles. The biphasic wave of evoked potentials in the MG muscles was followed by one or more oscillations, which were scarcely observed in the SOL muscles. These burst discharges and oscillations in the MG muscles [corrected] also occurred even after transection of the sciatic nerve proximal to the stimulating site. 3. A late component with a small amplitude could be observed in the evoked potentials of MG and SOL muscles. It was potentiated in amplitude after the administration of the drug. 4. Prolonged action of acetylcholine (ACh) has been known to occur in the presence of anticholinesterase drugs, and this was taken into account for the mechanism of the phenomena proposed in the present study. Contrasting changes between MG and SOL muscles were observed, which may be explained by different modes of ACh release in the MG and SOL muscles.

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Section: (A)mentioning

confidence: 99%

A Possible Interpretation for Difference in Neostigmine‐induced Changes of Spontaneous Activities and Evoked Muscle Potentials Between Rat Medial Gastrocnemius and Soleus Muscles

Uramoto

Watanabe

Totsuka

1993

Clin Exp Pharma Physio

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Modification of frequency augmentation‐potentiation by GTPγS in the frog neuromuscular junction

Enomoto

Maéno

1995

Cell Biochemistry & Function

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The effect of modifiers of guanine nucleotide-binding proteins (G proteins) on the frequency augmentation-potentiation of transmitter release were studied in the frog neuromuscular junction. Using Genetransfer as a carrier the mean quantal content of the endplate potential increased by penetration of GTP gamma S into the presynaptic nerve terminal. Neither GTP gamma S alone nor carrier alone had any effect. The relationship of log (mean quantal content) versus stimulation frequency changed from a single linear to a dual linear function, suggesting that the immediately releasable pool was modified. GDP beta S + carrier also had similar effects, but was less potent. Aluminium fluoride was without effect. Extracellularly recorded presynaptic nerve action potentials remained unchanged with GTP gamma S + carrier. Also, GTP gamma S + carrier did not affect the action potential nor the cytosolic Ca2+ concentration in differentiated NG108-15 hybrid cells. It is suggested that some smg-type G protein-dependent processes are involved in determining frequency augmentation-potentiation.

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Effects of vesicular acetylcholine uptake blockers on frequency augmentation-potentiation in frog neuromuscular transmission

Maéno

Enomoto

1994

Neuroscience

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Kinetic analyses of transmitter release in neuromuscular transmission

Cited by 5 publications

References 78 publications

A Possible Interpretation for Difference in Neostigmine‐induced Changes of Spontaneous Activities and Evoked Muscle Potentials Between Rat Medial Gastrocnemius and Soleus Muscles

A Possible Interpretation for Difference in Neostigmine‐induced Changes of Spontaneous Activities and Evoked Muscle Potentials Between Rat Medial Gastrocnemius and Soleus Muscles

Modification of frequency augmentation‐potentiation by GTPγS in the frog neuromuscular junction

Effects of vesicular acetylcholine uptake blockers on frequency augmentation-potentiation in frog neuromuscular transmission

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