Kinetic analysis of alcoholic fermentation using intermediate and by-products of sugar beet processing in laboratory bioreactor

Abstract

Bioethanol production in batch culture by free Saccharomyces cerevisiae cells from intermediate and by-products of sugar beet processing was investigated. This research relays on the previouslyconducted experiments in the bioreactor of 1.5 L working volumewhere key fermentation parameters, including initial sugar concentration and fermentation time, were optimized. This study was performed to validate the applicability of obtained results in the enlarged scale i.e. in 10 L laboratory bioreactor. The main aim was to analyze the bioprocess time course by monitoring the yeast cell number and concentrations of sugar, amino nitrogen, total soluble salts,and ethanol. Also, the kinetic analysis of ethanol and yeast biomass formation as well as sugar consumption during fermentation of media based on raw juice, thin juice, thick juice,and molasses was performed.