Kinetic Analysis of Distribution of Weak Acid in an Oil-Gelatin Microcapsule/Water System Studied by Microcapillary Injection and Microabsorption Methods

Nakatani, Kiyoharu; Kobayashi, Y.; Kakizaki, Hiroshi

doi:10.2116/analsci.20.1529

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…The distribution and release rates of ZnMb were measured by microcapillary manipulation/injection and microspectroscopy systems, as previously reported. ,− Several silica particles were immersed in 3 mL of a phosphate buffer solution (pH 6.5) in a glass-based dish (35 mm, AGC TECHNO GLASS Co., Ltd.) to fill the pores with the solution. The typical depth of the solution in the dish was 5 mm.…”

Section: Methodsmentioning

confidence: 99%

Kinetic Analysis of the Mass Transfer of Zinc Myoglobin in a Single Mesoporous Silica Particle by Confocal Fluorescence Microspectroscopy

et al. 2021

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The adsorption/desorption mechanisms of biomolecules in porous materials have attracted significant attention because of their applications in many fields, including environmental, medical, and industrial sciences. Here, we employ confocal fluorescence microspectroscopy to reveal the diffusion behavior of zinc myoglobin (ZnMb, 4.4 nm × 4.4 nm × 2.5 nm) as a spherical protein in a single mesoporous silica particle (pore size of 15 nm). The measurement of the time course of the fluorescence depth profile of the particle reveals that intraparticle diffusion is the rate-limiting process of ZnMb in the particle. The diffusion coefficients of ZnMb in the particle for the distribution (D dis ) and release (D re ) processes are determined from the rate constants, e.g., D dis = 1.65 × 10 −10 cm 2 s −1 and D re = 3.68 × 10 −10 cm 2 s −1 , for a 10 mM buffer solution. The obtained D values for various buffer concentrations are analyzed using the pore and surface diffusion model. Although surface diffusion is the main distribution process, the release process involves pore and surface diffusion, which have not been observed with small organic molecules; the mechanism of transfer of small molecules is pore diffusion alone. We demonstrate that the mass transfer kinetics of ZnMb in the silica particle can be explained well on the basis of pore and surface diffusion.

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Section: Methodsmentioning

confidence: 99%

Kinetic Analysis of the Mass Transfer of Zinc Myoglobin in a Single Mesoporous Silica Particle by Confocal Fluorescence Microspectroscopy

et al. 2021

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“…An absorption spectrum of the anthracene-9-carboxylic acid in single microparticles was also dependent on the pH in water (pK a = 2.5) [17]. For anthracene-9-carboxylic acid, the absorption band of the neutral species (HAC) overlapped with that of the dissociated anion (AC − ).…”

Section: Absorption Spectra Of Single Microparticlesmentioning

confidence: 99%

Mass transfer of weak acid in nanometer-sized pores of octadecylsilyl-silica gel

Kakizaki

Aonuma

Nakatani

2007

Journal of Colloid and Interface Science

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Diffusion behavior of rhodamine 6G in single octadecylsilyl-functionalized silica particle revealed by fluorescence correlation spectroscopy

Miyagawa,

Nohira,

Nagatomo

et al. 2024

ANAL. SCI.

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Kinetic Analysis of Distribution of Weak Acid in an Oil-Gelatin Microcapsule/Water System Studied by Microcapillary Injection and Microabsorption Methods

Cited by 3 publications

References 11 publications

Kinetic Analysis of the Mass Transfer of Zinc Myoglobin in a Single Mesoporous Silica Particle by Confocal Fluorescence Microspectroscopy

Kinetic Analysis of the Mass Transfer of Zinc Myoglobin in a Single Mesoporous Silica Particle by Confocal Fluorescence Microspectroscopy

Mass transfer of weak acid in nanometer-sized pores of octadecylsilyl-silica gel

Diffusion behavior of rhodamine 6G in single octadecylsilyl-functionalized silica particle revealed by fluorescence correlation spectroscopy

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