Kinetic Analysis of Drug‐Induced G₂ Block In Vitro

Abstract

The data on cell-cycle effects of two prospective antitumour agents, (+)-1,2,-bis(3,5-dioxopiperazine-l-yl)propane (soluble ICRF; NSC 169780) and 1,4-bis(2'chloroethyl)-1,4-diazabicyclo [2.2.1 I heptane diperchlorate (CBH; NSC 5 7 198) were used to determine whether a modified stathmokinetic experiment could predict the effects of continuous, long-term (0-48 hr) drug exposure in an in vitro

Branching Processes in Biology

“…The (G 2 + M) compartment also contains those G 2 cells which progressed to M but did not divide; instead, they increased their ploidy by, for example, defective cytokinesis. Simulation of the effects of continuous exposure to the drug based on this model was carried out analogously to similar simulations in Kimmel and Traganos (1985) or in Darzynkiewicz et al (1984); for a more theoretical treatment, see Kimmel (1980c).…”

Cell cycle analysis by flow cytometry: Principles and applications

“…Pharmacology and toxicology constitute an important field of biomedical applications for flow cytometry [137,149]. Monoparametric or multiparametric analysis of the cell cycle, as well as kinetic studies, can be interesting to evaluate in vitro drug effects [51,93]. It allows to emphasize the drug cytotoxicity, its specific phase of action [78], its influence on cellular metabolism or the position in the cycle where cells are killed [60].…”

“…Cycle analysis by flow cytometry is interesting in fundamental research but also in the biomedical field. In pharmacology, it allows in vitro tests for new drugs, such as antitumoral factors [6, 93,156], in order to develop new treatments. In oncology, cell DNA content and their distribution in the various cycle phases can be used to detect pathological cells [13], to establish prognosis [89,115] or to monitor treatments [7,160].…”