Kinetic analysis of microcalorimetric data derived from microbial growth: Basic theoretical, practical and industrial considerations

Cabadaj, Miroslav; Bashir, Shazia; Haskins, David; Said, Jawal; McCoubrey, Laura E.; Gaisford, Simon; Beezer, Anthony E.

doi:10.1016/j.mimet.2021.106276

Cited by 7 publications

(2 citation statements)

References 18 publications

(12 reference statements)

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“…The microbial growth process generally can be divided into five main phases: lag phase, logarithmic phase, stable phase, decline phase and death phase. In the logarithmic phase, total number of bacteria would multiply rapidly to the power of 2 n [11]. The process of bacteria binary fission was shown in Figure 2.…”

Section: Figure 2 the Process Of Bacteria Binary Fissionmentioning

confidence: 99%

Evaluation of Measurement Uncertainty from Pour Plate Method in Bacterial Enumeration in Water

Li¹,

Ma²,

Kong³

et al. 2022

AJEP

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The evaluation of measurement uncertainty (MU) is an important method for quantitative determinations derived by microbial cultivation, thus it could further improve the result accuracy. In this study, we aimed to evaluate the MU in total bacteria counts (TBC) in water. Experiments were carried out using Pour Plate Method. The bottom-up approach was used to access the MU of TBC and different mathematical models were set up based on microbial growth principles and test process. Two geometric and five non-geometric progression factors derived from fishbone diagram were selected in these models, especially considering the influence of the bacteria binary fission. The results showed that the calculated value of expanded uncertainty was between 82 and 120CFU/mL when the test result was 100CFU/mL with the 95% confidence level. The geometric progression factor had a greater impact on MU evaluation with almost 80% of contribution, whilst non-geometric progression factor dedicated 60%. Experimental procedures such as sample repeatability, reproducibility and inoculation time should be drawn more attention, thus the accuracy of results could be improved. These innovative models not only reasonable and reliable, but also easy to use. The achievements of this study illustrated that the MU evaluation method could also be applied for analyzing other microbial indicators in water.

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Section: Figure 2 the Process Of Bacteria Binary Fissionmentioning

confidence: 99%

Evaluation of Measurement Uncertainty from Pour Plate Method in Bacterial Enumeration in Water

Li¹,

Ma²,

Kong³

et al. 2022

AJEP

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“…Microcalorimetry is a quantifiable and accurate measurement method for biological growth energy. It detects the effect of drugs on cell metabolism by continuously measuring the energy changes produced by biological growth [ 31 , 32 ]. We have developed an approach using isothermal microcalorimetry to evaluate liquid-state microbial growth exothermic properties that include the enthalpy of growth [ 33 , 34 ], biological growth curve differences (including lag, logarithmic, stationary, and decline phases), and isothermal liquid-state growth properties.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity effects of electrolytically generated hypochlorous acid-treated pathogenic microorganisms by isothermal kinetic simulation

Tsai

Liu

Lin

et al. 2022

J Therm Anal Calorim

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This study involves isothermal kinetic simulation to evaluate the parameters of inhibition conditions for Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) of high-risk pathogens. This is because the new type of the 2019 novel coronavirus (2019-nCoV) is continuously spreading and the importance of public health issues. Environmental disinfection and personal wearing of masks have become important epidemic prevention measures. Selection of concentration kinetics could be estimated best for E. coli and S. aureus of pathogens, 2.74 × 10 4 and 10 5 and 2.44 × 10 4 and 10 5 colony-forming units (CFU mL –1 ), by isothermal micro-calorimeter (TAM Air) tests, respectively. Comparisons were made of different doses of 0–70 ppm (in 20 mL test ampoule) hypochlorous acid treatment for conducting n th-order and autocatalytic reaction simulation to evaluate the inhibition reaction parameters, which determined the autocatalytic kinetic model that was beneficially applied on the E. coli and S. aureus . We developed the inhibition reaction parameters of the pathogens, which included the activation energy ( E a ), the natural logarithm of pre-exponential factor (ln k 0 ), the enthalpy of inhibition microbial growth reaction ( ∆H ), inhibition microbial growth, and the inhibition growth analysis. Overall, we conducted isothermal kinetic simulation to understand the antimicrobial activity effects of electrolytically generated hypochlorous acid-treated pathogenic microorganisms, which will provide reference for public health and medical-related fields for SDG3, and can contribute to ensuring human health and hygiene.

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Enhancement of sulfide removal and sulfur recovery in piggery wastewater via lighting-anaerobic digestion with bioaugmentation of phototrophic green sulfur bacteria

Jirasansawat,

Chiemchaisri,

Chiemchaisri

2024

Environ Sci Pollut Res

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Kinetic analysis of microcalorimetric data derived from microbial growth: Basic theoretical, practical and industrial considerations

Cited by 7 publications

References 18 publications

Evaluation of Measurement Uncertainty from Pour Plate Method in Bacterial Enumeration in Water

Evaluation of Measurement Uncertainty from Pour Plate Method in Bacterial Enumeration in Water

Antimicrobial activity effects of electrolytically generated hypochlorous acid-treated pathogenic microorganisms by isothermal kinetic simulation

Enhancement of sulfide removal and sulfur recovery in piggery wastewater via lighting-anaerobic digestion with bioaugmentation of phototrophic green sulfur bacteria

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