Kinetic Analysis of Ozonation Degree Effect on the Physicochemical Properties of Ozonated Vegetable Oils

Guerra-Blanco, P.; Chairez, Isaac; Poznyak, Tatyana; Brito-Arias, Marco

doi:10.1080/01919512.2020.1868972

Cited by 9 publications

(7 citation statements)

References 36 publications

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“…Moureu et al observed that the acid value also correlated with antimicrobial activity [33]. In addition, Guerra-Blanco et al observed that anti-inflammatory and wound-healing effects were achieved with ozonated grape seed and sunflower oil with a low degree of ozonation, implying that the superior therapeutic effect of ozonated oils is not necessarily due to a high peroxide value [36].…”

Section: Discussionmentioning

confidence: 99%

“…The ozonated oils used in this study (linseed, hemp seed, sunflower, and olive oil) were specifically prepared for research purposes and were generously provided by UAB Ozono Centras, Lithuania. Oils were ozonated for 4 h without water, and the ozonation time was chosen after analysing the results of other scientists' research [30,36,37]. This was the minimum time during which good antimicrobial activity results are usually obtained.…”

Section: Methodsmentioning

confidence: 99%

“…However, Guerra-Blanco et al confirmed that the ozonation kinetics of different oils (grapeseed, sunflower, avocado, and olive) differ [36]. Therefore, such an analysis of several different oils would help to gain a broader picture of the characteristics of ozonated oils and to find an optimal formulation suitable for the treatment of skin infection in animals that would be an alternative to the use of antibiotics and the prevention of antibiotic resistance.…”

Section: Animal Speciesmentioning

confidence: 99%

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A Comparative Study of the Chemical Properties and Antibacterial Activity of Four Different Ozonated Oils for Veterinary Purposes

Slavinskienė,

Grigonis,

Ivaškienė

et al. 2024

Veterinary Sciences

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Infectious skin diseases are quite common in veterinary medicine. These diseases can be caused by both bacteria and pathogenic fungi. Antimicrobial drugs are usually used for treatment. An alternative to these drugs could be ozonated oils with antibacterial and antifungal properties. Four different ozonated oils (linseed, hemp seed, sunflower, and olive) were tested in order to develop an optimal pharmaceutical form for the treatment of skin infections in animals. Chemical parameters such as acid and acidity value, iodine and peroxide value, viscosity, and infrared spectres were analysed. The ozonation of oils resulted in changes in their chemical composition. The antimicrobial activity of the tested oils was evaluated by determining the minimum inhibitory concentrations and zones of inhibition in agar. After ozonation, the acid content increased in all the tested oils. The highest acidity was found in linseed oil (13.00 ± 0.11 mg KOH/g; 6.1%). Hemp oil, whose acidity was also significant (second only to linseed oil), was the least acidified by ozonation (11.45 ± 0.09 mg KOH/g; 5.75%). After ozonation, the iodine value in oils was significantly reduced (45–93%), and the highest amounts of iodine value remained in linseed (47.50 ± 11.94 g Iodine/100 g oil) and hemp (44.77 ± 1.41 Iodine/100 g oil) oils. The highest number of peroxides after the ozonation of oils was found in sunflower oil (382 ± 9.8 meqO2/kg). It was found that ozonated hemp and linseed oils do not solidify and remain in liquid form when the temperature drops. The results showed a tendency for the reference strains of S. aureus, E. faecalis, and E. coli to have broader zones of inhibition (p < 0.001) than clinical strains. Overall, ozonated linseed oil had the highest antibacterial activity, and ozonated olive oil had the lowest, as determined by both methods. It was found that ozonated linseed oil was the most effective on bacteria, while the most sensitive were S. aureus ATCC 25923, MRSA, and S. pseudointermedius (MIC 13.5 mg/mL, 4.6 mg/mL, and 13.5 mg/mL, respectively, and sterile zones 20.67 ± 0.98 mm, 20.25 ± 0.45 mm, and 18.25 ± 0.45 mm, respectively). The aim and new aspect of this work is the characterisation of selected ozonated vegetable oils, especially hemp oil, according to chemical and antibacterial parameters, in order to select suitable candidates for preclinical and clinical animal studies in the treatment of bacterial or fungal skin infections in terms of safety and efficacy.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Animal Speciesmentioning

confidence: 99%

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A Comparative Study of the Chemical Properties and Antibacterial Activity of Four Different Ozonated Oils for Veterinary Purposes

Slavinskienė,

Grigonis,

Ivaškienė

et al. 2024

Veterinary Sciences

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“…The reaction of ozone with oil or ozonation of oil containing unsaturated fatty acids leads to the formation of oxygenated compounds such as hydroperoxides, ozonides, and aldehydes, which reduce bacterial infections in wounds. The ozonation process is widely applied to various types of oil, both for medical, energy and other purposes, such as kapuk oil [9], rice bran oil [10], sunflower oil [11][12][13], olive oil [14][15][16][17][18][19][20], vegetable oil [21][22], palm seed oil [23], waste cooking oil [24] and bulk frying oil [25].…”

Section: Introductionmentioning

confidence: 99%

Increasing Peroxide Number of Sumbawa Oil Through Ozonation Process Using Double Dielectric Barrier Discharge (DDBD) Plasma Reactor

Puryadi,

Hilmi,

Ulfa

2024

J. Pijar.MIPA

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Oil is one of the most popular media for the ozonation process compared to other media such as water or saline methods or direct exposure to blood. Ozonated oil has an advantage over other ozone therapy methods because ozonated oil has an antiseptic activity that is several hundred times higher than ozonated saline. Sumbawa oil is one of the local wisdom products of the Sumbawa tribal community, which is generally used as medicine or massage therapy to heal injuries such as broken bones, tendonitis, bruises, back pain and treatment of various skin wounds. An ozonation process can be carried out to increase the ability of Sumbawa oil to regenerate skin and repair tissue. Ozonation uses a Double Dielectric Barrier Discharge (DDBD) plasma reactor. The DDBD Plasma Reactor is a reactor that can produce ozone gas with very high purity. Pure ozone gas is very well applied in the medical world. This research aims to increase the peroxide levels in Sumbawa oil by ozonation using a Double Dielectric Barrier Discharge (DDBD) Plasma reactor. The flow rates used were 0.1 L/minute, 0.4 L/minute and 0.7 L/minute for 1 hour, 3 hours and 5 hours. The results showed that the peroxide value increased with increasing oxygen flow rate and ozonation time. The highest peroxide value was obtained at a flow rate of 0.7 L/minute for 5 hours, namely 332.03 mg Eq/kg Sumbawa oil. Increased by 282.71 mg Eq/kg; the peroxide figure for Sumbawa oil without ozonation was 49.32 mg Eq/kg.

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“…Due to the high concentration of unsaturated fatty acids in RBO, such as oleic and linoleic, it may be used as a raw material for the creation of trioxolane and peroxide as an active medicinal component through the Ozonation process with Criegee mechanism (Criegee,1975), which is beneficial in the body's fight against free radicals, as shown in Figure 1. Guerra-Blanco et al (2021) created ozonated vegetable oil from a variety of vegetable oils and investigated their kinetic response; moreover, the research discovered that the density and viscosity of the ozonated vegetable oil varied significantly. Previous study from RBO's NMR test suggested that the 1,2,4-trioxalane group was still searching for and obtaining only its derivative compounds, mainly aldehydes and peroxides (Enjarlis et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Effect of pH and Ozone Dosage on Characteristic of Ozonated Rice Bran Oil

Enjarlis

Christwardana

Handayani

et al. 2022

molekul

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The influence of pH and ozone dose, as well as ascorbic acid addition during the ozonation process, on the properties of Rice Bran Oil (RBO), was examined. The spectroscopic characteristic of RBO before and after ozonation was analysis directly, while the physicochemical property was assessed by density, viscosity, pH, iodine number, peroxide number, and acid number. With an increase in ozone dose, the carbon double bond in the RBO reduced. The primary product of the ozonation process is ozonide, and one of its by-products is 1,2,4-trioxolane, which contains a carbon single bond as a result of the ozonation reaction. According to this study, the pH 4 and ozone dose of 440 mg O3/L are the optimum parameters utilized in the RBO ozonation process. RBO's density and viscosity were 0.918 g/mL and 0.042 cP, respectively, at these conditions. Its iodine number, acid number, and peroxide number were also 3.173 g/g RBO, 2.3 mg NaOH/g RBO, and 55 mgeq/kg, respectively. Analyses of gas chromatography and nuclear magnetic resonance spectroscopy revealed the presence of 1,2,4-trioxolane. Ozone dosage is critical because greater ozone concentrations place RBO in a saturated state, making the 1,2,4-trioxolane unstable and readily destroyed, whereas lower temperatures can avoid this.

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Kinetic Analysis of Ozonation Degree Effect on the Physicochemical Properties of Ozonated Vegetable Oils

Cited by 9 publications

References 36 publications

A Comparative Study of the Chemical Properties and Antibacterial Activity of Four Different Ozonated Oils for Veterinary Purposes

A Comparative Study of the Chemical Properties and Antibacterial Activity of Four Different Ozonated Oils for Veterinary Purposes

Increasing Peroxide Number of Sumbawa Oil Through Ozonation Process Using Double Dielectric Barrier Discharge (DDBD) Plasma Reactor

Effect of pH and Ozone Dosage on Characteristic of Ozonated Rice Bran Oil

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