Kinetic analysis of Penicillin production by semicontinuous fermenters

The dynamic analysis of a continuous, aerobic, fixed-film bioreactor has been performed. Rigorous mathematical models have been developed for a fluidized-bed fermentor with biofilm growth. The transient performance of the reactor is appraised in terms of outlet penicillin concentration for constant, as well as variable carbon substrate feed rates. The effect of the reactor oxygen transfer capacity is elucidated for those cases employing substrate feeding strategies. The results show that penicillin production in a continuous, fixed-film bioreactor reaches a maximum with processing time, but subsequently decreases as cell mass accumulates and substrate deficiencies occur. The maximum production level can be maintained for increased operating times if the substrate supply is continuously increased. The duration of this prolonged production is a direct function of the rate of increase and the operating time at which the increase is initiated. The oxygen transfer capacity of the reactor was found to be important to the effectiveness of a feeding strategy.

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“…where qm is the mean value of q (7) evaluated over 0 5 7 5 1 . For a constant substrate feed to the reactor, qnI is approximated by (see ref.…”

Section: _ --!?Amentioning

confidence: 99%

Analysis of a continuous, aerobic, fixed‐film bioreactor. II. Dynamic behavior

Park

Davis

Wallis

1984

Biotech & Bioengineering

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“…The feeding law of sugar solution has been assumed as an independent variable; the functions z(t), P (1), and the withdrawals depend on such a law. In this analysis the law of nutrient addition has been obtained a posteriori, i.e., either the function p(t) or C,(t) has been fixed and then the time dependence of the sugar solution flow rate has been deduced.…”

Section: Simulation Of the Operationmentioning

confidence: 99%

The Influence of oxygen concentration and of specific rate of growth on the kinetics of Penicillin production

Giona¹,

Santis²,

Marrelli³

et al. 1976

Biotech & Bioengineering

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The role of fundamental parameters in the conduction of penicillin semicontinuous fermentations is analyzed. Biomass concentration, penicillin production, and main nutrient consumption are particularly studied. Furthermore, the conduction of the operation is simulated with regard to conditions of constant specific rate of growth and of constant oxygen concentration in the broth. An intermediate condition is also considered.

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Application of balancing methods in modeling the penicillin fermentation

Heijnen

Roels

Stouthamer

1979

Biotech & Bioengineering

110

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This paper shows the application of elementary balancing methods in combination with simple kinetic equations in the formulation of an unstructured model for the fed-batch process for the production of penicillin. The rate of substrate uptake is modeled with a Monod-type relationship. The specific penicillin production rate is assumed to be a function of growth rate. Hydrolysis of penicillin to penicilloic acid is assumed to be first order in penicillin. In simulations with the present model it is shown that the model, although assuming a strict relationship between specific growth rate and penicillin productivity, allows for the commonly observed lag phase in the penicillin concentration curve and the apparent separation between growth and production phase (idiophase-trophophase concept). Furthermore it is shown that the feed rate profile during fermentation is of vital importance in the realization of a high production rate throughout the duration of the fermentation. It is emphasized that the method of modeling presented may also prove rewarding for an analysis of fermentation processes other than the penicillin fermentation.

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Kinetic analysis of Penicillin production by semicontinuous fermenters

Cited by 15 publications

References 19 publications

Analysis of a continuous, aerobic, fixed‐film bioreactor. II. Dynamic behavior

Analysis of a continuous, aerobic, fixed‐film bioreactor. II. Dynamic behavior

The Influence of oxygen concentration and of specific rate of growth on the kinetics of Penicillin production

Application of balancing methods in modeling the penicillin fermentation

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