Kinetic analysis of the Fenton reaction by ESR‐spin trapping

Mizuta, Yukio; Masumizu, Toshiki; Kohno, Masahiro; Mori, Akitane; Packer, Lester

doi:10.1080/15216549700204931

Cited by 16 publications

(19 citation statements)

References 10 publications

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“…Due to its hydrophobicity, heme molecule intercalates into lipid environments, enhancing the possibility of reaction with lipid peroxides, which can be decomposed into peroxyl and alkoxyl radicals (Aikens and Dix, 1991;Buettner, 1993;Van der Zee et al, 1996;Mizuta et al, 1997). Binding of heme to lipoproteins and phospholipid membranes results in oxidation of lipids and has been implicated in a broad range of pathological states, such as atherosclerosis or hemolytic diseases (Chou and Fitch, 1981;Hebbel and Eaton, 1989;Balla et al, 1991;Schmitt et al, 1993).…”

Section: Introductionmentioning

confidence: 96%

HeLp, a heme-transporting lipoprotein with an antioxidant role

Maya‐Monteiro

Alves

Pinhal

et al. 2004

Insect Biochemistry and Molecular Biology

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Section: Introductionmentioning

confidence: 96%

HeLp, a heme-transporting lipoprotein with an antioxidant role

Maya‐Monteiro

Alves

Pinhal

et al. 2004

Insect Biochemistry and Molecular Biology

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“…(1–5) Spin-trapping agents can react with short-lived free radicals, such as • OH, superoxide (O 2 •− ) and other organic radicals to make a relatively stable nitroxyl radical form, the so-called spin adduct. 5,5-Dimethyl-1-pyrroline- N -oxide (DMPO) is probably the most common spin-trapping agent.…”

Section: Introductionmentioning

confidence: 99%

Method for assessing X-ray-induced hydroxyl radical-scavenging activity of biological compounds/materials

Ueno¹,

Nakanishi²,

Matsumoto³

2013

J. Clin. Biochem. Nutr.

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A method for correctly assessing hydroxyl radical scavenging activity of antioxidative chemicals and/or biological compounds/ materials was proposed. This method can simultaneously assess two factors, i.e. hydroxyl radical scavenging and 5,5 dimethyl 2 hydroxy 1 pyrrolidine N oxide (hydroxyl radical adduct of 5,5 dimethyl 1 pyrroline N oxide) reducing ability, as antioxidative properties. In this paper, some biologically common hydrophilic molecules, cell culture media, and rat plasma were tested. X ray induced hydroxyl radical can be detected using the electron para magnetic resonance spin trapping technique. Using X ray irradiation of the reaction mixture as the hydroxyl radical source, the true hydroxyl radical scavenging ability of the subjected antioxidant can be assessed. In addition, the method simultaneously measures the reduction of 5,5 dimethyl 2 hydroxy 1 pyrrolidine N oxide, to estimate the reducing ability of the antioxidant. Biological materials, such as sugars and proteins, could abolish hydroxyl radical at the biological concentration. Ascorbic acid showed reducing ability at the biological concentration. The simultaneous assessment of hydroxyl radical scavenging and reducing ability of antioxidants can be an informative index for antioxidants.

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“…The spin concentration of NO-NIF-derived radicals was determined by the double integration using Bruker WinEPR software, and radical concentrations were calculated by reference to the double integral of signals from a known concentration of the freshlyprepared stable radical Tempol (0-0.1 mM) run under identical conditions, as described previously. 30) HUVEC-derived ROS formation was studied by DMPO spin trapping using the methods of Souchard et al with slight modifications.…”

Section: )mentioning

confidence: 99%