Kinetic Analysis of Tumor Cells in Bone and Soft Tissue Sarcomas.

Kinetic Analysis of Malignant Fibrous Histiocytoma Cells Treated with Anticancer Agents In Vivo.

“…As for the application of the present results to clinical treatment, we have already reported that the potential doubling time of bone and soft tissue sarcomas in nude mice is a useful prognostic indicator, despite differences in histologic diagnoses [15]. It should be noted that neither the Tc nor Ts values reflect the over all status of the tumor cells.…”

“…Since this double-labeling procedure has a time lag of 2 hr, the proportion (dS) of cells not in the DNA synthesis phase during the double-labeling experiments could be derived from a stain sample. The values of Ts and Tc were therefore calculated on the basis of the dS [15,19,20,22]. In brief, they were calculated as follows: dS = blue/(brown + double) Ts =time lag/dS Tc=Ts/LI x growth fraction The growth fraction was considered to be an approximate value of the PCNA labeling index (PCNA-LI), and it was obtained as follows.…”

“…These cell cycle parameters can also be calculated while the histologic architecture of the tumor specimen is preserved. We have already reported that potential doubling time can be a prognostic factor in patients with bone and soft tissue sarcomas [15].…”