Kinetic Analysis on Cell Growth and Biosynthesis of Poly (3-Hydroxybutyrate) (PHB) in Cupriavidus Necator H16

Khan, Maksudur R.; Prasad, D. M. Reddy; Abdullah, Huda; Batcha, Abeed Fatima Mohidin

doi:10.7763/ijbbb.2013.v3.268

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…Therefore; it is possible that saturated fatty acids are more readily taken up and converted to acetyl-CoA than unsaturated fatty acids. Generally, cell growth and PHB accumulation were in the expected range and the %PHB for date seed oil is greater than that achieved by Khan et al, [44] using Jatropha oil and also compares favourably to those reported by Kamilah et al, [9] using waste cooking oil.…”

Section: Necator Growth and Phb Production In Shake Flasks With Date Seed Oilsupporting

confidence: 72%

Waste date seed oil extract as an alternative feedstock for Poly(3-hydroxybutyrate) synthesis

Yousuf

Winterburn

2017

Biochemical Engineering Journal

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Section: Necator Growth and Phb Production In Shake Flasks With Date Seed Oilsupporting

confidence: 72%

Waste date seed oil extract as an alternative feedstock for Poly(3-hydroxybutyrate) synthesis

Yousuf

Winterburn

2017

Biochemical Engineering Journal

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“…Growth-associated production means that the rate of polymer production parallels the bacterial growth rate. Many studies confirmed the growth associated nature of PHB production where Khan et al (2013)…”

Section: Kinetic Modelingmentioning

confidence: 86%

Scaling up, Kinetic modeling, and Economic analysis of poly (3-hydroxybutyrate) production by Bacillus cereus isolate CCASU-P83

Elgendy

Aboshanab

Yassien

et al. 2021

Archives of Pharmaceutical Sciences Ain Shams University

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Polyhydroxyalkanoates (PHA) are environmentally friendly polymers produced by many bacteria under nutrientlimited conditions. However, their commercialization is hindered by production expenses. The present study aimed at cost-effective and efficient production of poly(3-hydroxybutyrate) (PHB) by Bacillus (B.) cereus isolate CCASU-P83. Through one factor at time optimization study on shake flask, B. cereus CCASU-P83 produced about 50 % polymer per dry weight after 48 hours incubation time. For better evaluation of the fermentation process, kinetic modeling using the Logistic and Leudking-piret models was applied. A preliminary economic analysis was carried out and leads to a 30.8 % reduction in the total cost. In comparison to the findings obtained on the level of shake flask, scaling up to the bioreactor resulted in producing about 53% PHB per dry weight after only 24 hours incubation. These models concluded that B. cereus produced PHB during the growth phase. Analysis of molecular weight of the produced polymer displayed a 26900 g/mole molecular weight with a polydispersity index (PDI) of 1.1. In conclusion, B. cereus CCASU-P83 is a potential candidate for industrial production of PHB polymer using corn oil in a short incubation period which highly reduced the cost of the production process.

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“…Samples were taken periodically to measure biomass, carbohydrate, and PHA concentrations. Experiments were run during 72 h, based on C. necator H16 accumulation reports, which do not report a significant increase in accumulation after 60 h, under batch conditions [ 23 ]. For comparison, parallel fermentations from the same seed culture were performed with the mineral medium described added with 15 g/L fructose as the carbon source.…”

Section: Methodsmentioning

confidence: 99%

Production of polyhydroxybutyrate by coupled saccharification–fermentation of inulin

Guzmán-Lagunes,

Martínez-dlCruz,

Wongsirichot

et al. 2023

Bioprocess Biosyst Eng

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Inulin is a fructose-based polysaccharide that can be found in several plant species, from grass and onions to chicory roots; thus, it has the potential to be an excellent renewable source of fructose for several industrial applications. Among them, inulin hydrolysis can be coupled to a fermentation operation to produce polyhydroxybutyrate (PHB) using Cupriavidusnecator H16. This work reports the PHB production process involving chicory root inulin hydrolysis using inulinase Novozym 960 followed by a C.necator fermentation. It was found that the maximum saccharification (95% wt.) was reached at 269 U/ginulin after 90 min. The hydrolysates obtained were then inoculated with C.necator, leading to a biomass concentration of 4 g/L with 30% (w/w) polymer accumulation. Although PHB production was low, during the first hours, the cell growth and polymer accumulation detected did not coincide with a fructose concentration decrease, suggesting a simultaneous saccharification and fermentation process, potentially alleviating the product inhibition inherent to the inulinase–fructose system. The characterization of the obtained PHB showed a polymer with more homogeneous values of Mw, and better thermal stability than PHB produced using pure fructose as a fermentation substrate. The results obtained demonstrate a viable alternative carbon substrate for PHB production, opening the possibility for inulin-rich renewable feedstock valorization.

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Kinetic Analysis on Cell Growth and Biosynthesis of Poly (3-Hydroxybutyrate) (PHB) in Cupriavidus Necator H16

Cited by 4 publications

References 14 publications

Waste date seed oil extract as an alternative feedstock for Poly(3-hydroxybutyrate) synthesis

Waste date seed oil extract as an alternative feedstock for Poly(3-hydroxybutyrate) synthesis

Scaling up, Kinetic modeling, and Economic analysis of poly (3-hydroxybutyrate) production by Bacillus cereus isolate CCASU-P83

Production of polyhydroxybutyrate by coupled saccharification–fermentation of inulin

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