Kinetic and Thermodynamic Parameters for Heat Denaturation of Cry1A(b) Protein from Transgenic Maize (<i>Zea mays</i>)

Luis, Ruth de; Pérez, María Dolores; Sánchez, Lourdes; Lavilla, María; Calvo, Miguel

doi:10.1111/j.1750-3841.2008.00798.x

Cited by 11 publications

(13 citation statements)

References 19 publications

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“…When comparing the macro- and micronutrient composition of the four experimental diets (the non-GM, mGM, sGM and smGM diets; Table 6), these were compositionally very similar. The transgenic protein Cry1Ab was detected in the GM maize and EPSPS in the GM soya (three out of three samples positive for both), but all diet samples were negative, presumably due to denaturation of protein during heat treatment of the diets ( 55 ) . The reference diet had higher protein and energy levels and a lower residue content (Table 6); this was unavoidable as we wanted to include high levels of the GM plant materials.…”

Section: Resultsmentioning

confidence: 99%

Zebrafish (Danio rerio) as a model for investigating the safety of GM feed ingredients (soya and maize); performance, stress response and uptake of dietary DNA sequences

Sissener¹,

Johannessen

Hevrøy³

et al. 2009

Br J Nutr

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A 20-d zebrafish (Danio rerio) feeding trial, in which a near doubling of fish weight was achieved, was conducted with GM feed ingredients to evaluate feed intake, growth, stress response and uptake of dietary DNA. A partial aim of the study was to assess zebrafish as a model organism in GM safety assessments. Roundup Ready w soya (RRS w ), YieldGard w Bt maize (MON810) and their non-modified, maternal, near-isogenic lines were used in a 2 £ 2 factorial design. Soya variety and maize variety were the main factors, both with two levels; non-GM and GM. Compared with fish fed non-GM maize, those fed GM maize exhibited significantly better growth, had lower mRNA transcription levels of superoxide dismutase (SOD)-1 and a tendency (non-significant) towards lower transcription of heat shock protein 70 in liver. Sex of the fish and soya variety had significant interaction effects on total RNA yield from the whole liver and transcription of SOD-1, suggesting that some diet component affecting males and females differently was present in different levels in the GM and the non-GM soya used in the present study. Dietary DNA sequences were detected in all of the organs analysed, but not all of the samples. Soya and maize rubisco (non-transgenic, multicopy genes) were most frequently detected, while MON810 transgenic DNA fragments were detected in some samples and RRS w fragments were not detected. In conclusion, zebrafish shows promise as a model for this application.

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Section: Resultsmentioning

confidence: 99%

Zebrafish (Danio rerio) as a model for investigating the safety of GM feed ingredients (soya and maize); performance, stress response and uptake of dietary DNA sequences

Sissener¹,

Johannessen

Hevrøy³

et al. 2009

Br J Nutr

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“…The transgenic protein Cry1Ab was not detected in the diets, probably due to denaturation during heat treatment of the feeds (De Luis et al, 2008). This would possibly exclude effects of the Cry1Ab-toxin, but there might also be other unintended differences between the GM and non-GM maize.…”

Section: Stress Response and Seawater Transfermentioning

confidence: 99%

A long term trial with Atlantic salmon (Salmo salar L.) fed genetically modified soy; focusing general health and performance before, during and after the parr–smolt transformation

Sissener¹,

Sanden²,

Bakke³

et al. 2009

Aquaculture

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First and foremost, I would like to thank my main supervisor Gro-Ingunn Hemre for excellent supervision, support and encouragement along the way. Monica Sanden also made valuable contributions, both in planning, practical work and fruitful discussions during the "GMO-morning coffees". Anne-Katrine Lundebye Haldorsen and Ernst Hevrøy gave constructive feedback in the writing process of the thesis. Also, thanks to all my co-authors on the papers for their efforts, constructive criticism and invaluable input for the papers of this thesis. Thanks to Dr. Sam Martin for accepting our invitation for collaboration, for organizing my very pleasant and rewarding stay in Aberdeen, and to both him and Dr. Phil Cash for advice and input during the proteomics work. Evelyn Argo gave excellent day-today technical assistance with the gel work, being extremely helpful, patient and always smiling. Also thanks to Elizabeth Stewart for help with the protein identifications. Much appreciated technical assistance here at NIFES was provided by Jaap Wessels, Eva Mykkeltvedt, Leikny Fjeldstad and Elin Krohnstad. Thanks to Ivar Helge Matre and Lise Dyrhovden technical assistance during the salmon feeding trial at the Institute of Marine Research, Matredal. Also I would like to thank all the other PhD students and other colleagues at NIFES for their support, and for all the good times and social events outside of work.

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“…The Cry1Ab, Cry1Ac, NPTII, and PAT (bar) proteins expressed in the three CTC sugarcane varieties studied herein have undergone various thermostability studies which were intended to mimic food processing conditions. 16,17 For example, de Luis et al 18,19 reported that heating (nixtamalization) of maize grain containing Cry1Ab at 85°C for 60 minutes produced a 70% loss of detectable immunoreactive protein whereas a 90% reduction occurred after 3 minutes when heated in porridge at 75°C. 19 Susceptibility to denaturation at a temperature of 75-77°C was also shown in studies of maize leaf extracts where 50-70% of immunoreactive protein was lost in just 2 min.…”

Section: Discussionmentioning

confidence: 99%

“…This filter cake is usually returned to sugarcane fields as fertilizer. 7: The clarified juice (14)(15)(16)(17)(18)(19)(20), is concentrated by vacuum evaporation, at an initial temperature of approximately 110°C which is then, with simultaneous increase of vacuum, decreased to 85-90°C. When syrup of around 65°Brix is produced, this evaporation process in concluded.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the effects of sugarcane processing on the presence of GM DNA and protein in sugar

et al. 2020

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The Brazilian Sucro-energy Sector produces both energy, in the form of ethanol fuel, industrial steam and electricity, and sugar. Centro de Tecnologia Canavieira (CTC), the leading Brazilian sugarcane breeding company, has developed a pipeline of insect-protected sugarcane varieties to control sugarcane borer damage. The goal of this manuscript is to present the results of studies with three genetically modified (GM) sugarcane varieties and to evaluate the published literature regarding the possible presence of GM sugarcane DNA or protein in raw or refined sugar. Specifically, two varieties of approved GM sugarcane, CTC91087-6 and CTC175-A, and an experimental CTC variety, were grown in four individual plots to produce four batches each of processed raw sugar using standard smaller-scale laboratory processing methods resulting in a total of 12 independent batches of raw sugar. Herein, we report the development of event-specific probes and DNA detection methods, designed to detect the junction of sugarcane genomic DNA and the inserted DNA of the two approved GM varieties. An identical approach was used for the testing of sugar made from the experimental CTC variety. The methodology used TaqMan® real-time PCR and ELISA assays validated for the four GM proteins expressed by these three events (Cry1Ab, Cry1Ac, NPTII, and PAT (bar)). The developed assays had very low limits of detection (LODs) for the various event-specific DNA probes (7.2-25 ng/g sugar) and insecticidal and selectable marker proteins (2.9-10.9 ng/g sugar). No event-specific DNA and no GM proteins were detectable in the 12 independent batches of raw sugar produced from these three GM sugarcane events. The results of this study, using very sensitive methods and testing several sugar batches, extend the conclusions of previous studies, reviewed herein, that showed the extensive degradation and removal of DNA and protein during sugarcane processing. Overall, these results indicate that there are no distinguishable differences between the highly purified, chemically defined sugar produced from conventional or GM varieties.

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Kinetic and Thermodynamic Parameters for Heat Denaturation of Cry1A(b) Protein from Transgenic Maize (Zea mays)

Cited by 11 publications

References 19 publications

Zebrafish (Danio rerio) as a model for investigating the safety of GM feed ingredients (soya and maize); performance, stress response and uptake of dietary DNA sequences

Zebrafish (Danio rerio) as a model for investigating the safety of GM feed ingredients (soya and maize); performance, stress response and uptake of dietary DNA sequences

A long term trial with Atlantic salmon (Salmo salar L.) fed genetically modified soy; focusing general health and performance before, during and after the parr–smolt transformation

Evaluation of the effects of sugarcane processing on the presence of GM DNA and protein in sugar

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