Kinetic and Thermodynamic Spectrophotometric Technique to Estimate Gabapentin in Pharmaceutical Formulations using Ninhydrin

Siddiqui, Farhan Ahmed; Sher, Nawab; Shafi, Nighat; Shamshad, Hina; Zubair, Arif

doi:10.1186/2093-3371-4-17

Cited by 10 publications

(7 citation statements)

References 33 publications

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“…The reaction of GBP and ninhydrin was shown in Figure 2. [43][44][45] To determine the wavelength of maximum absorption by this method, GBP solution with concentration of 60 μg mL −1 was prepared. Then, 1 mL of mentioned solution was reacted with 2 mL of ninhydrin solution with concentration of 0.2% (w/v) in methanol.…”

Section: Gbp Release Characteristicmentioning

confidence: 99%

Dual Drug Release from Gelatin/PLGA Core–Shell Fibers for Diabetic Neuropathic Wound Healing

Mostofizadeh

Ghasemi‐Mobarakeh

Zamani

2021

Macro Materials & Eng

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Diabetes mellitus is one of the most common chronic diseases affecting many people around the world. One of the most common complications of diabetes is diabetic neuropathic pain (DNP), which affects about 10-26% of diabetic patients. In this research, the gelatin/poly(lactic-co-glycolic acid) (PLGA) core-shell fibers containing gabapentin (GBP) and ciprofloxacin hydrochloride (CipHCl) are fabricated via coaxial electrospinning for healing of diabetic neuropathic wounds. The core-shell structure of fibers is confirmed using transmission electron microscopy (TEM) and fluorescence microscopy. Furthermore, scanning electron microscopy (SEM) is utilized to explore the morphology of core-shell fibers, revealing the smaller diameter for core-shell fibers containing CipHCl and GBP compared to the core-shell fibers without the drugs. X-ray diffraction (XRD) represents the amorphous distribution of drugs inside the core-shell fibers, which is also confirmed by differential scanning calorimetry (DSC). Core-shell fibers containing CipHCl and GBP show strong antimicrobial properties against both Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) bacteria. The in vitro drug release studies demonstrate the sustained release of CipHCl and GBP within 64 days from the core-shell fibers. In conclusion, the fabricated core-shell fibers show a great promise to be utilized as an efficient dressing for diabetic neuropathic wounds.

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Section: Gbp Release Characteristicmentioning

confidence: 99%

Dual Drug Release from Gelatin/PLGA Core–Shell Fibers for Diabetic Neuropathic Wound Healing

Mostofizadeh

Ghasemi‐Mobarakeh

Zamani

2021

Macro Materials & Eng

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“…In this regard, direct analysis has been fraught with major disadvantages. Due to this deficiency, several methods have been previously reported for the determination of GBP in dosage forms and these methods include: spectrophotometric [8][9][10][11][12][13][14][15], spectrofluorimetric [16,17], capillary electrophoresis [18], potentiometric [19], HPLC [20][21][22] and HPTLC with derivatization using ninhydrin [23]. Majority of the spectrophotometric and spectrofluorimetric methods, as well as some previously reported HPLC techniques, involve prior derivatization with a variety of reagents.…”

Section: Introductionmentioning

confidence: 99%

New spectrophotometric method for the determination of gabapentin in bulk and dosage forms using p-dimethylaminobenzaldehyde

Adegoke

Adegbolagun

Aiyenale

et al. 2018

Journal of Taibah University for Science

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A new simple, accurate and economic spectrophotometric method based on azo dye derivatization for the determination of gabapentin (GBP) was developed. Critical factors were optimized. The method was validated and assay of dosage forms was done. Spot tests and TLC confirmed the formation of azo adduct. A 0.3 M NaNO 2 solution using 2 M HCl was used for diazotization. The optimal temperature and time were 30°C and 10 min. Azo adducts were determined at 430 nm. Methanol was found to be the best solvent. Gabapentin coupled at a ratio of 1:1 with DMAB. The assays of GBP were linear over the range 1-6 µg/mL (r = 0.9973) and LOD of 0.8322 µg/mL. The methods were accurate (error < 2%) and precise (RSD < 0.5%). The methods were successfully applied to the assay of GBP in dosage forms and compared favorably with reference method (p > .05). The successful diazotization of gabapentin and the azo adduct formation with DMAB is reported for the first time.

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“…A glance over the literature revealed few reports regarding charge transfer complexes for PG and TXA. A structural analogous of PG and TXA gabapentin has been determined by colorimetry-visible spectrophotometry by reacting with iodine, chloranil, chloranilic acid, 2,3-dichloro-5,6-dicyano-1,4-benzoquinone, tetracyanoethylene, methyl orange, hydroxy benzaldehyde, picric acid and with ninhydrin [ 28 – 30 ]. Colorimetric spectrophotometric methods for PG include reaction with 1,2-naphthoquinone-4-sulphonate sodium and 2,4-dinitrofluorobenzene [ 5 ], ascorbic acid and salicylaldehyde [ 6 ], 7,7,8,8-tetracyanoquinodimethane, 2,3-dichloro-5,6-dicyano-1,4-benzoquinone, 2,5-dichloro-3,6-dihydroxy-1,4-benzoquinone, tetracyanoethylene and 2,3,5,6-tetrachloro-1,4-benzoquinone [ 7 ], quinalizarin and alizarin [ 8 ], and vanillin, acetyl acetone, and formaldehyde [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Pregabalin and Tranexamic Acid Evaluation by Two Simple and Sensitive Spectrophotometric Methods

Sher

Fatima

Perveen

et al. 2015

International Journal of Analytical Chemistry

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This paper demonstrates colorimetric visible spectrophotometric quantification methods for amino acid, namely, tranexamic acid and pregabalin. Both drugs contain the amino group, and when they are reacted with 2,4-dinitrophenol and 2,4,6-trinitrophenol, they give rise to yellow colored complexes showing absorption maximum at 418 nm and 425 nm, respectively, based on the Lewis acid base reaction. Detailed optimization process and stoichiometric studies were conducted along with investigation of thermodynamic features, that is, association constant and standard free energy changes. The method was linear over the concentration range of 0.02–200 µgmL−1 with correlation coefficient of more than 0.9990 in all of the cases. Limit of detection was in range from 0.0041 to 0.0094 µgmL−1 and limit of quantification was in the range from 0.0137 to 0.0302 µgmL−1. Excellent recovery in Placebo spiked samples indicated that there is no interference from common excipients. The analytical methods under proposal were successfully applied to determine tranexamic acid and pregabalin in commercial products. t-test and F ratio were evaluated without noticeable difference between the proposed and reference methods.

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Kinetic and Thermodynamic Spectrophotometric Technique to Estimate Gabapentin in Pharmaceutical Formulations using Ninhydrin

Cited by 10 publications

References 33 publications

Dual Drug Release from Gelatin/PLGA Core–Shell Fibers for Diabetic Neuropathic Wound Healing

Dual Drug Release from Gelatin/PLGA Core–Shell Fibers for Diabetic Neuropathic Wound Healing

New spectrophotometric method for the determination of gabapentin in bulk and dosage forms using p-dimethylaminobenzaldehyde

Pregabalin and Tranexamic Acid Evaluation by Two Simple and Sensitive Spectrophotometric Methods

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