Kinetic Characterization of Apical D-Fructose Transport in Chicken Jejunum

Garriga, Carles; Barfull, A.; Planas, Joana M.

doi:10.1007/s00232-003-0640-0

Cited by 11 publications

(10 citation statements)

References 28 publications

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“…According to the NCBI Gene database [32,37], orthologs of glut5/slc2a5 are found so far in 123 organisms across chicken, dog, cow, chimpanzee, Rhesus monkey, mouse, rat and X. tropicalis . Chicken GLUT5 has been shown to have mRNA expression in the small intestine [53] and may be regulated by glucocorticoids [54]. …”

Section: Glut Transporter Classesmentioning

confidence: 99%

Avian and Mammalian Facilitative Glucose Transporters

2017

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The GLUT members belong to a family of glucose transporter proteins that facilitate glucose transport across the cell membrane. The mammalian GLUT family consists of thirteen members (GLUTs 1–12 and H+-myo-inositol transporter (HMIT)). Humans have a recently duplicated GLUT member, GLUT14. Avians express the majority of GLUT members. The arrangement of multiple GLUTs across all somatic tissues signifies the important role of glucose across all organisms. Defects in glucose transport have been linked to metabolic disorders, insulin resistance and diabetes. Despite the essential importance of these transporters, our knowledge regarding GLUT members in avians is fragmented. It is clear that there are no chicken orthologs of mammalian GLUT4 and GLUT7. Our examination of GLUT members in the chicken revealed that some chicken GLUT members do not have corresponding orthologs in mammals. We review the information regarding GLUT orthologs and their function and expression in mammals and birds, with emphasis on chickens and humans.

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Section: Glut Transporter Classesmentioning

confidence: 99%

Avian and Mammalian Facilitative Glucose Transporters

2017

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“…SGLT1-like proteins responsible for intestinal sugar uptake have been detected, and in some instances cloned and/or functionally characterized, in all vertebrate groups (4,31,33,44), while the identification of GLUT1-like transporters is extremely limited (26,48,49). GLUT5-like transporters have been studied and detected only in birds (22). In invertebrates, Na ϩ -dependent sugar transport (3,16,30,53,57) or SGLT1-like proteins (14) have been described in parasitic platetyhelminths, as well as snail and decapod crustaceans.…”

Section: The Adult Females Of the Entomophagous Parasitoid Waspmentioning

confidence: 99%

Unexpected similarity of intestinal sugar absorption by SGLT1 and apical GLUT2 in an insect (Aphidius ervi,Hymenoptera) and mammals

Caccia¹,

Casartelli²,

Grimaldi³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

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Sugars are critical substrates for insect metabolism, but little is known about the transporters and epithelial routes that ensure their constant supply from dietary resources. We have characterized glucose and fructose uptakes across the apical and basolateral membranes of the isolated larval midgut of the aphid parasitoid Aphidius ervi. The uptake of radiolabeled glucose at the basal side of the epithelium was almost suppressed by 200 microM cytochalasin B, uninhibited by phlorizin, and showed the following decreasing rank of specificity for the tested substrates: glucose > glucosamine > fructose, with no recognition of galactose. These functional properties well agree with the expression of GLUT2-like transporters in this membrane. When the apical surface of the epithelium was also exposed to the labeled medium, a cation-dependent glucose uptake, inhibited by 10 microM phlorizin and by an excess of galactose, was detected suggesting the presence in the apical membrane of a cation-dependent cotransporter. Radiolabeled fructose uptakes were only partially inhibited by cytochalasin B. SGLT1-like and GLUT5-like transporters were detected in the apical membranes of the epithelial cell by immunocytochemical experiments. These results, along with the presence of GLUT2-like transporters both in the apical and basolateral cell membranes of the midgut, as we recently demonstrated, allow us to conclude that the model for sugar transepithelial transport in A. ervi midgut appears to be unexpectedly similar to that recently proposed for sugar intestinal absorption in mammals.

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“…In the present study, the complete suppression of fructose-sustained EPSPs by CCB indicates that a hexose transporter sensitive to CCB is responsible for fructose utilization in the hippocampus. Additionally, CCB does not appear to inhibit GLUT5-mediated hexose uptake (Burant et al, 1992; Garriga et al, 2004). Taken together, these observations raise the possibility that fructose utilization in the CNS is mediated by a hexose transpoter distinct from GLUT5 and that other glucose transporters in CNS are involved in fructose uptake.…”

Section: Discussionmentioning

confidence: 99%

Glial–neuronal interactions underlying fructose utilization in rat hippocampal slices

Izumi

Zorumski

2009

Neuroscience

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Although fructose is commonly used as a sweetener, its effects on brain function are unclear. Using rat hippocampal slices, we found that fructose and mannose, like pyruvate, preserve ATP levels during 3-hours of glucose deprivation. Similarly, fructose and mannose restored synaptic potentials (EPSPs) depressed during glucose deprivation. However, restoration of synaptic responses was slow and only partial with fructose. EPSPs supported by mannose were inhibited by cytochalasin B (CCB), a glucose transport inhibitor, but were not inhibited by α-cyano-4-hydroxycinnamate (4-CIN), a monocarboxylate transport inhibitor, indicating that neurons use mannose via glucose transporters. In contrast, both CCB and 4-CIN depressed EPSPs supported by fructose, suggesting that fructose may be taken up by non-neuronal cells through CCB sensitive hexose transporters and metabolized to a monocarboxylate for subsequent use during neuronal respiration. Supporting this possibility, twenty minutes of oxygen deprivation in the presence of fructose resulted in functional and morphological deterioration whereas oxygen deprivation in the presence of glucose or mannose had minimal toxic effects. These results indicate that neuronal fructose utilization differs from glucose and mannose and likely involves release of monocarboxylates from glia.

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Kinetic Characterization of Apical D-Fructose Transport in Chicken Jejunum

Cited by 11 publications

References 28 publications

Avian and Mammalian Facilitative Glucose Transporters

Avian and Mammalian Facilitative Glucose Transporters

Unexpected similarity of intestinal sugar absorption by SGLT1 and apical GLUT2 in an insect (Aphidius ervi,Hymenoptera) and mammals

Glial–neuronal interactions underlying fructose utilization in rat hippocampal slices

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