Kinetic characterization of methylthio-d-ribose-1-phosphate isomerase

Veeramachineni, Vamsee; Ubayawardhana, Subashi T; Murkin, Andrew S.

doi:10.1016/bs.mie.2023.03.015

Cited by 2 publications

(2 citation statements)

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“…Thirty larvae from five new replicate exposures to each compounds’ intermediate concentration of 5 mg/L were collected following an adapted methodology described by Gusso and Wiprich [ 26 ], Veeramachineni, Ubayawardhana, and Murkin [ 74 ], and Kodama, Fukui, and Kometani [ 75 ]. The larvae were collected in 200 µL of tris-citrate buffer (50 mM Tris, 2 mM EDTA, 2 mM EGTA, pH 7.4) and the samples were centrifuged at 4 °C for 10 min at 800× g .…”

Section: Methodsmentioning

confidence: 99%

“…4.4.2. 5 ′ -Ectonucleotidase (AMP Hydrolysis) and NTPDase (ADP and ATP Hydrolysis) Activities Thirty larvae from five new replicate exposures to each compounds' intermediate concentration of 5 mg/L were collected following an adapted methodology described by Gusso and Wiprich [26], Veeramachineni, Ubayawardhana, and Murkin [74], and Kodama, Fukui, and Kometani [75]. The larvae were collected in 200 µL of tris-citrate buffer (50 mM Tris, 2 mM EDTA, 2 mM EGTA, pH 7.4) and the samples were centrifuged at 4 • C for 10 min at 800× g. The resulting supernatant was collected and subjected to a second centrifugation at 4 • C for 10 min at 21,000× g. The remaining pellets were then frozen at −80 • C, thawed, resuspended in 200 µL of tris-citrate buffer, and used for total protein quantification at 280 nm using a Power Wave XS2 microplate reader (Bio-Tek Instruments, USA).…”

Section: Biochemical Analysis 441 Ache Activitymentioning

confidence: 99%

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Antinociceptive Analysis of Natural Monoterpenes Eugenol, Menthol, Carvacrol and Thymol in a Zebrafish Larval Model

Rocha,

Félix,

Monteiro

et al. 2024

Pharmaceuticals

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In the last decade, a considerable number of studies have broadened our knowledge of the nociceptive mechanisms of pain, a global health problem in both humans and animals. The use of herbal compounds such as eugenol, menthol, thymol, and carvacrol as analgesic agents has accompanied the growing interest in this area, offering a possible solution for this complex problem. Here, we aimed to explore how these natural substances—at three different concentrations (2, 5 and 10 mg/L)—affect the pain responses in zebrafish (Danio rerio) larvae exposed to 0.05% acetic acid (AA) for 1 min. By analysing the activity of acetylcholinesterase (AChE), 5′-ectonucleotidase and NTPDases, as well as aversion and exploratory behaviours, it was observed that that although all substances were effective in counteracting the pain stimulus, the concentration range within which they do so might be very limited. Eugenol, despite its acknowledged properties in fish anaesthesia, failed to alleviate the pain stimulus at low concentrations. Contrastingly, menthol exhibited the most promising results at the lowest concentrations tested. Overall, it is concluded that menthol might be a good analgesic for this species, qualifying it as a substance of interest for prospective studies.

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Section: Methodsmentioning

confidence: 99%